ABSTRACT
The present study concentrated on the sacculant portion of the kidney of B. glabrata as a hemopoietic stem cell. The histological picture of this portion in the kidney as well as in vitro culture was studied. The histological studies showed that the sacculant portion constituted the last fifth of the kidney highly folded with primitive epithelial tissue, amoebocytes [10.7 +/- 0.98 m] rounded, oval or ameboid, showing flattening and spreading when encapsulated around foreign particles, when doing so, both the cytoplasm and nucleus were more basophilic. The hemocytes in the sacculant portion were distributed either as clustering forming amebocytic plugs or dense aggregation or being normal in distribution. There was a significant correlation between hemocytic diameter and shell weight and between shell weight and shell diameter, but no significant correlation between hemocytic diameter and shell diameter. The in vitro studies showed that the medium 199 and fetal calf serum [3: 1] produced higher number of granulocytes. The cells differed in shape and size from those shown in histological studies of the kidney itself. The majority of the cells were large sized granulocytes and very few small sized hyalinocytes. Granulation of cytoplasm took place in the culture medium used more than in the sacculant portion, only the smaller granulocytes showed greater ability for mitotic division. The results were photographed and discussed
Subject(s)
Kidney/anatomy & histologyABSTRACT
Ptyodactylus guttatus and Tarentola annularis are sympatric throughout most of their geographical distribution in Egypt. Their temporal activities, feeding habits, food items, feeding strategy are almost similar. These species harbored six species of nematodes, one species of trematodes, in addition to helminthic cysts of cestodes in the liver of each specific host