ABSTRACT
The huge amount of information, the big number of scientists and their efforts, labs, man/hrs, fund, companies all and others factors build the success of the amazing new branch of genetic engineering the 'Protein Engineering' [PE]. It concerns with the modification of protein structure/function[s] or building protein from scratch. The engineered proteins usually have new criteria [s]. Engineering proteins can be mediated on the level of genes or proteins. PE fined its way in different important sectors including industrial, pharmaceutical and medicinal. Aspects about PE and its applications will be discussed with this review. The concept, tools, and the industrial applications of the protein, engineered proteins and PE will be under focus. In order to get up to date Knowledge about the applications of PE in basic protein and molecular biology, several examples are discussed. PE can play a significant role in different industrial and pharmaceutical sectors if used wisely and selectively
ABSTRACT
Pseudomonas aeruginosa recognized as opportunistic pathogen causes severe infections for hospitalized patients, survive in and resist many antimicrobial agents like antibiotics and disinfectants. The aim of this study is to evaluate the role of EDTA in improving the sensitivity of resistant P. aeruginosa strains to disinfectants and Na-citrate. The strains used in this study were selected in house from Tanta University hospital, Egypt and tested for the synergistic effect of EDTA with Na-citrate or disinfectant (s). The results showed a significant effect ofEDTA in improving P. aeruginosa sensitivity. In conclusion, we proposed that using EDTA in combination with different sanitization compounds and antimicrobial agentsespecially in hospitals aiming to control the spreading of infections.