ABSTRACT
<p><b>OBJECTIVE</b>Evaluating the accuracy and safety as well as the equivalence compared with the control kit of RIDA QUICK Norovirus detection kit(R-Biopharm, Germany).</p><p><b>METHODS</b>Based on the results of commercially available IDEA Norovirus detection kit (ELISA), the sensitivity and specificity and accuracy of RIDA QUICK Norovirus detection kit (immunochromatographic assay) were evaluated.</p><p><b>RESULTS</b>The sensitivity and specificity of RIDA QUICK Norovirus detection kit were 98.4% and 92.4%, and the accuracy was 97.6% compared with the control kit.</p><p><b>CONCLUSION</b>RIDA QUICK Norovirus detection kit has good sensitivity and specificity for the detection of norovirus antigens.</p>
Subject(s)
Humans , Enzyme-Linked Immunosorbent Assay , Methods , Chromatography, Affinity , Methods , Norovirus , Reagent Kits, Diagnostic , Sensitivity and SpecificityABSTRACT
<p><b>BACKGROUND</b>In our previous studies, we found the expression of 14-kD phosphohistidine phosphatase (PHPT1) was associated with lung cancer cells migration and invasion, and PHPT1 mRNA expression level in lung cancer tissues clinically correlated with lymph node metastasis. In the present study, we aimed to further investigate the expression of PHPT1 protein in lung cancer.</p><p><b>METHODS</b>Expression of PHPT1 protein in tissue samples from 146 lung cancers and 30 normal tissues adjacent to lung cancers was assessed using immunohistochemical method. Fisher's exact test was used to analyze expression patterns of PHPT1 protein in these tissue types. Meanwhile, we studied the correlation between expression of PHPT1 protein and clinicopathological features in lung cancer.</p><p><b>RESULTS</b>Significantly higher expression levels of PHPT1 protein were found in lung cancer samples (53.42%) than in normal tissues adjacent to lung cancer (23.33%) (P = 0.003). Fisher's exact test showed that lung cancer stage positively correlated with expression of PHPT1 protein (P = 0.02), and lung cancer samples with lymph node metastasis showed higher PHPT1 protein expression (P = 0.016) than the samples without lymph node metastasis.</p><p><b>CONCLUSIONS</b>The results of this study agree with findings from our previous study of PHPT1 mRNA expression in lung cancer tissues, and strongly suggest that PHPT1 protein is closely associated with the carcinogenesis and metastasis of lung cancer. Thus, therapy targeting PHPT1 (inhibition or silencing) could be potentially benefited for lung cancer patients.</p>
Subject(s)
Humans , Blotting, Western , Gene Expression Regulation, Neoplastic , Immunohistochemistry , In Vitro Techniques , Lung Neoplasms , Metabolism , Phosphoric Monoester Hydrolases , MetabolismABSTRACT
The Toxoplasma gondii vaccine is the best way to prevent Toxoplasma gondii infection. The improvement of the biological techology promots the study of Toxoplasma gondii vaccine. The newest study progresses of Toxoplasma gondii genetic engineering vaccine,which is summarized on the basis of the current study results,is respected to provide new aspects of the Toxoplasma gondii vaccine study for the researches.