The transcriptional regulation study on human delta globin gene with CAAT box C-->T point mutation in its promoter / 中国医学科学院学报

<p><b>OBJECTIVE</b>To study the transcriptional regulation of human delta globin gene with C-->T point mutation at -64 in its promoter.</p><p><b>METHODS</b>Human delta globin genes including wild CAAT box and mutant CAAT box (-64C-->T) were separately cloned into eukaryotic expression vector pcDNA3.1 (-)/Myc-His A which was cut out the strong promoter CMV, transfected MEL cells, and induced by DMSO to express. The transcriptional regulation of human delta globin gene was analysed using semi-quantitative RT-PCR.</p><p><b>RESULTS</b>The expression level of human delta globin gene with mutant CAAT box was 2.2-fold as high as that with wild CAAT box.</p><p><b>CONCLUSION</b>The defective CAAT box of human delta globin gene promoter region may be one of the major reasons for its low expression level.</p>