ABSTRACT
Background: Bone marrow mesenchymal stem cells [BM-MSCs] elicit neuroprotective effects, and their repair ability has been investigated in different experimental models. We aimed to investigate the effect of multiple i.p. BM-MSCs injections in the cuprizone model of multiple sclerosis in mice
Methods: Adult male C57BL/6 mice [n = 40] were fed a regular diet or a diet containing cuprizone [0.2% w/w] for six weeks. Bone marrow samples were taken from patients with spinal cord injury. BM-MSCs [2 * 10[6] in 1 milliliter medium] were administered intraperitoneally for two consecutive weeks at the end of the forth weeks of cuprizone administration. Animals [n = 12] were perfused with 10% paraformaldehyde at the end of sixth week. The brains were sectioned coronally in 6- 8-Mu m thickness [-2.3 to 1.8 mm from bregma]. The sections were stained by luxol fast blue-cresyl violet, and images were captured via a microscope. Demyelination ratio was estimated in corpus callosum in a blind manner. A quantitative real-time PCR was used to measure the myelin basic protein gene expression at sixth week
Results: Histologically, cuprizone induced demyelination in the corpus callosum. Demyelinated area was diminished in the corpus callosum of cell-administered group. Cuprizone could decrease myelin-binding protein mRNAs expression in corpus callosum, which was significantly recovered after BM-MSCs injections
Conclusion: Our data indicated a remyelination potency of multiple i.p. BM-MSCs in the cuprizone model of multiple sclerosis in mice
ABSTRACT
Evaluation of the effect of Propolis as a bioactive material on quality of dentin and presence of dental pulp stem cells. For conducting this experimental split-mouth study, a total of 48 maxillary and mandibular incisors of male guinea pigs were randomly divided into an experimental Propolis group and a control calcium hydroxide group. Cutting the crowns and using Propolis or calcium hydroxide to cap the pulp, all of the cavities were sealed. Sections of the teeth were obtained after sacrificing 4 guinea pigs from each group on the 10th, 15th and 30th day. After they had been stained by hematoxylin and eosin [H and E], specimens underwent a histological evaluation under a light microscope for identification of the presence of odontoblast-like cells, pulp vitality, congestion, inflammation of the pulp and the presence of remnants of the material used. The immunohistochemistry [IHC] method using CD[29] and CD[146] was performed to evaluate the presence of stem cells and the results were statistically evaluated by Kruskal-Wallis, Chi Square and Fisher tests. In H and E stained specimens, there was no difference between the two groups in the presence of odontoblast-like cells, pulp vitality, congestion, inflammation of the pulp and the presence of remnants of used material[p>0.05]. There was a significant difference between the quality of regenerative dentin on the 15[th] and 30[th] days [p<0.05]: all of the Propolis cases presented tubular dentin while 14% of the calcium hydroxide cases produced porous dentin. There was no significant difference between Propolis and calcium hydroxide in stimulation of dental pulp stem cells [DPSCs]. This study which is the first one that documented the stimulation of stem cells by Propolis, provides evidence that this material has advantages over calcium hydroxide as a capping agent in vital pulp therapy. In addition to producing no pulpal inflammation, infection or necrosis this material induces the production of high quality tubular dentin