Efeito do treinamento sobre a cinemática de equinos no salto de obstáculos / Effects of training on kinematic traits of jumping horses

Este trabalho teve como objetivo avaliar o efeito do treinamento sobre as variáveis da cinemática de equinos jovens da raça Brasileiro de Hipismo no salto em liberdade. Foram utilizados 14 equinos da raça Brasileiro de Hipismo com idade de 40 a 42 meses, peso vivo de 436,3±11,6kg e 448,2±12,7kg e altura na cernelha de 1,57±0,03m e 1,58±0,03m ao início e ao final do experimento, respectivamente. Os equinos realizaram dois protocolos de avaliação de salto, um antes e outro após o treinamento. O treinamento teve a duração de cinco meses e consistiu em 50 minutos de atividade física por dia, montados, sendo dois dias de flexionamento, dois dias de condicionamento físico e dois dias de trabalho específico de salto de obstáculos. Nos protocolos de avaliação, foram filmados cinco saltos em liberdade sobre um obstáculo oxer, com o primeiro e o segundo elemento na altura de 0,90m e 1,00m de altura, respectivamente, e 0,90m de largura. Foram fixados marcadores reflexivos nos equinos em pontos anatômicos utilizados como pontos de referência para a avaliação das características cinemáticas no salto. As filmagens foram realizadas com câmera de 100Hz, e as imagens, processadas no Simi Reality Motion Systems (r). Os resultados das variáveis avaliadas foram comparados com o teste de t pareado (P<0,05). O treinamento resultou no aumento das variáveis (P<0,05): velocidade da passada anterior ao salto, velocidade do salto, deslocamento horizontal da cernelha no salto, ângulo escapuloumeral, distância vertical entre as articulações escapuloumeral e metacarpofalângica, ângulo coxofemoral e ângulo femorotibial. No entanto, os valores dos ângulos umerorradial e tibiometatarsiano apresentaram redução após o treinamento (P>0,05). Conclui-se que o treinamento específico para salto com cinco meses de duração altera algumas variáveis cinemáticas dos equinos no salto de obstáculo proporcionando melhoria, principalmente, na trajetória do salto...

This study was carried out to evaluate the effect of training on the kinematic traits of young Brazilian Sport Horse in free jumping. 14 Brazilian Sport Horse were used, aged between 40 and 42 months, with body weight of 436.3±11.6kg and 448.2±12.7kg and height withers of 1.58±0.03m and 1.58±0.03m at the beginning and end of training, respectively. Two jumping evaluation protocols were used: before and after training. The training had the duration of five months and consisted of 50 minutes of exercise activities by day with a rider; two days of flexing, two days of physical conditioning and two days of specific jumping exercises. The evaluations were carried out, and five free jumps in each evaluation were record in an oxer, with the front fence of 0.90m and the back fence of 1.00m height and 0.90m of width. The horse's anatomical points were highlighted by reflective markers. The recordings were performed with 100Hz camera and the images processed in Simi Reality Motion Systems(r). The results were analyzed as paired data (P<0.05). The training increased the traits kinematics (P<0.05): last stride velocity speed prior to jumping, jumping velocity, horizontal withers displacement at jumping, scapulohumeral angle, vertical distance between scapulohumeral and metacarpophalangeal joint, coxofemoral angle and femorotibial angle. However, the kinematic traits reduced with training: humeroradial angle and tibiometatarsal angle (P<0.05). In conclusion, the jumping training with a five month duration changed some kinematic traits of free jumping in horses, especially the jumping trajectory...

Partial purification of an endoglucanase from Biomphalaria glabrata

Previous studies have shown that Biomphalaria glabrata contains a complete cellulolytic system which includes an endoglucanase, an exoglucanase and a ß-glucosidase. In the present report, a scheme for the purification of the endoglucanase from this invertebrate is proposed. Two major problems were encountered during the study: 1) the presence of a green-brownish pigment, which could not be eliminated by thermal shock or ammonium sulfate precipitation and 2) relative instability of enzymatic activity. Various alternatives were tested and the best sequence of steps was: 1) a sample of the crude extract, obtained by homogenization of the digestive glands in 50 mM Tris-HCl buffer, pH 8.4, and ultracentrifugation, was applied to a Q-Sepharose FPLC column (50 mM Tris-HCl buffer, pH 8.4; 10 mm x 22.2 cm column; flow rate 1.5 ml/min; 0.1 to 0.5 M NaCl gradient); 2) the eluate peak containing activity was dialyzed, lyophilized and eluted from a Superdex-75 gel filtration FPLC column (50 mM ammonium acetate buffer, pH 4.8; 16 mm x 60 cm column; flow rate 1.0 ml/min). A low degree of purification (about 36-fold) and recovery (about 12 percent) were observed, probably due to enzyme instability. SDS-electrophoresis of the active fraction showed a major peak of 30 kDa. In order to improve the purification scheme, further studies are required to stabilize this enzyme during purification and storage