Lectin-binding properties of Aeromonas caviae strains / Propriedades lectínicas de amostras de Aeromonas caviae

The cell surface carbohydrates of four strains of Aeromonas caviae were analyzed by agglutination and lectin-binding assays employing twenty highly purified lectins encompassing all sugar specificities. With the exception of L-fucose and sialic acid, the sugar residues were detected in A. caviae strains. A marked difference, however, in the pattern of cell surface carbohydrates in different A. caviae isolates was observed. Specific receptors for Tritricum vulgaris (WGA), Lycopersicon esculentum (LEL) and Solanum tuberosum (STA) (D-GlcNAc-binding lectins) were found only in ATCC 15468 strain, whereas Euonymus europaeus (EEL, D-Gal-binding lectin) sites were present exclusively in AeQ32 strain, those for Helix pomatia (HPA, D-GalNAc-binding lectin) in AeC398 and AeV11 strains, and for Canavalia ensiformes (Con A, D-Man-binding lectin) in ATCC 15468, AeC398, AeQ32 and AeV11 strains, after bacterial growing at 37ºC. On the other hand, specific receptors for WGA and EEL were completely abrogated growing the bacteria at 22ºC. Binding studies with 125I- labeled lectins from WGA, EEL and Con A were performed. These assays essentially confirmed the selectivity, demonstrated in the agglutination assays of these lectins for the A. caviae strains.

Os carboidratos de superfície celular de quatro amostras de Aeromonas caviae foram analisados por aglutinação e ensaios de ligação de lectinas empregando vinte lectinas altamente purificadas com especificidade para açúcares. Com exceção da L-fucose e do ácido siálico, os resíduos de açúcar foram detectados em amostras de A. caviae. Entretanto, foi observada uma diferença marcante no padrão de carboidratos de superfície celular em diferentes amostras de A. caviae. Receptores específicos para Tritricum vulgaris (WGA), Lycopersicon esculentum (LEL) e Solanum tuberosum (STA), lectinas de ligação a D-GlcNAc, foram encontrados apenas na amostra ATCC 15468, enquanto sítios de Euonymus europaes (EEL), lectina de ligação a D-Gal, estavam presentes exclusivamente na amostra AeQ32, sítios de Helix pomatia (HPA), lectina de ligação a D-GalNac, nas amostras AeC398 e AeV11 e de Canavalia ensiformis (Com A), lectina de ligação a D-Man, nas amostras ATCC 15468, AeC398, AeQ32 e AeV11, após crescimento bacteriano a 37ºC. Por outro lado, receptores específicos para WGA e EEL foram completamente abolidos após o crescimento das bactérias a 22ºC. Estudos de ligação com lectinas WGA, EEL e Con A marcadas com 125I também foram realizados. Esses ensaios confirmaram a seletividade, demonstrada em ensaios de aglutinação dessas lectinas para as amostras de A. caviae.

Co-infecção de sífilis e papilomavívus humano: interrompendo uma cadeia de transmissão vertical / Co-infecção between syphilis and human papilomavirus: broken a vertical transmission chain

Um paciente do sexo masculino, 26 anos, branco, casado, com o nível de escolaridade fundamental, residente no município de Duque de Caxias, Rio de Janeiro, foi atendido no Ambulatório do Instituto de Dermatologia Rubem David Azulay na Santa Casa de Misericórdia do Rio de Janeiro, em março de 2005, com queixa inicial de “verruga” peniana clássica, sugestiva, pela avaliação clínica, de um caso de HPV. Foi solicitada então como exame complementar a testagem sorológica para sífilis, aconselhada a realização do exame para detecção de anticorpos para o vírus HIV e foi sugerido também que ele trouxesse a sua parceira sexual para avaliação clínica. A realização do teste não treponêmico de sífilis, o VDRL (Veneral Disease Research Laboratory), foi reator 1:16, sendo confirmado pelo teste treponêmico TPHA (Treponema pallidum Hemaglutination Assay). O paciente não voltou para a consulta agendada, só retornando, cinco meses depois, acompanhado da esposa grávida de cinco meses. Ambos foram tratados de acordo com os protocolos do Ministério da Saúde. Na análise desse caso foram evidenciadas várias facetas das co-infecções das doenças sexualmente transmissíveis (DST).

A patient, male, 26 years old, married, with elementary school ship, lived in Duque de Caxias, Rio de Janeiro, Brazil, came for the clinical of Sexually Transmited Disease (STD) in Rubem David Azulay Dermatology Institute in Santa Casa de Misericórdia, Rio de Janeiro, with the principal complaint of a wart in his penis. After the first clinical evaluation, it was concluded that it was a case of human papilomavirus, the HPV. He was solicited complementary exams to detect syphilis and he was advised to make a test to detect antibody for HIV, he was also suggested that it was important to bring his wife in the next consult for evaluation. When the syphilis test of VDRL (Veneral Disease Research Laboratory) was realized it was detected a title of 1:16, it was confirmed with T.P.H.A.( Treponema pallidum Hemaglutination Assay), a treponema test. Unfortunately the patient didn’t come in the following consult, only regressing five months later. In this occasion he brought his wife, she was five months of pregnancy. Both of them was treated in accord of the protocol of the Health Ministry. In this Case of Study it was evidenced the relationship of co-infection in the sexually transmitted disease (STD).

Evidence for the presence of ß-D-galactofuranosyl residues (1->3)-linked to a-D-mannopyranose on the cell membrane in all developmental stages of Trypanosoma cruzi

The lipopeptidophosphoglycan (LPPG) component isolated from epimastigote forms of Trypanosoma cruzi has antigenic properties. Using a rabbit antiserum aginst LPPG, specific for ß-D-galactofuranosyl residues (1-3)-linked to alfa-Dmannopyranose, we identified, by indirect immunofluorescence, the presence of epitopes containing ß-D-galactofuranosyl structures on the cell membrane of epimastigote, amastigote and trypomastigote forms of the parasite. The results demonstrade that all developmental stages of T. cruzi contain similar antigenic determinants on their cell body and flagellar membrane