ABSTRACT
Na história natural do carcinoma de pênis, o envolvimento loco regional é considerado a complicação mais grave e principal causa de morte da doença. Compreender os processos moleculares que possam estar envolvidos nos mecanismos de invasão e disseminação metastática pode levar a um aprimoramento das ferramentas de prognóstico para uma melhor avaliação dos pacientes, evitando-se, assim, a morbidade de cirurgias desnecessárias. Alguns estudos têm enfatizado a influência da adesão celular no desenvolvimento tumoral e metastático, sendo as proteínas do complexo CD44/ERM (Ezrina, Radixina e Moesina) relatadas como envolvidas nestes processos. Objetivos: Analisar a co-expressão de CD44- Ezrina e de CD44-Moesina no carcinoma epidermóide de pênis, correlacionando esta co-expressão aos critérios clínico patológicos. Casuística, Materiais e Métodos: Foram estudados, retrospectivamente 76 pacientes submetidos à cirurgia para carcinoma epidermóide de pênis no AC Camargo Cancer Center. A avaliação das expressões isoladas de CD44, CD44V6, Ezrina e Moesina foi realizada por imunoistoquímica, no equipamento Ventana®, e a imunorreatividade de cada proteína foi categorizada em positiva e negativa. As co-expressões de Ezrina/CD44, Ezrina/CD44V6, Moesina/CD44 e Moesina/CD44V6 foram avaliadas...
The loco regional involvement is considered the most severe complication and main cause of death in the natural history of penile carcinoma. The understanding of molecular processes that may be involved in the invasion mechanism and metastatic dissemination would improve the prognostic tools to a better patient evaluation, avoiding the morbidity of unnecessary surgery. Some studies have emphasized the influence of cellular adhesion in the tumor and metastatic development, being the complex CD44/ERM proteins (Ezrin/Radixin/Moesin), reported as involved in these processes. Objectives: To analyze the co-expression of CD44-Ezrin and CD44-Moesin in penile squamous cell carcinoma, correlating this co-expression to pathological clinical criteria. Samples, Materials and Methods: We retrospectively studied 76 patients undergoing surgery for penile squam...
Subject(s)
Humans , Cell Adhesion , Carcinoma , Penile NeoplasmsABSTRACT
In this study we investigated the larval dispersal associated with larval predation in experimental populations of Chrysomya albiceps and Cochliomyia macellaria. Frequency distribution of sampling units (G test) in the substrate was used to evaluate variation in larval dispersal. An experimental acrylic channel (1 x 0.1 x 0.2 m) covered with wood shavings was used to observe larval dispersal prior to pupation. The acrylic channel was graduated at 0.05 m intervals, each representing a sampling unit; hence, 20 sampling units were set up. A Petri dish containing third instar larvae of single and double species was deposited at one edge of the acrylic channel allowing larvae to disperse. The number of buried pupae (0, 1, 2, àn) present in each sampling unit was recorded. For double species, the number of recovered larvae of C. albiceps was similar to the number initially released on the dish Petri. On the other hand, the number of recovered larvae of C. macellaria was significantly smaller than the initially released number. The results show that C. albiceps attacks C. macellaria larvae during the larval dispersal process. The larval distribution of C. albiceps did not differ significantly from C. macellaria in double species, but it differed significantly in single species. The larval aggregation level of C. macellaria decreased when C. albiceps was present and the larval aggregation level of C. albiceps increased when C. macellaria was present. The implications of such findings for the population dynamics of these species are discussed