Comparative Studies on the Physicochemical Characteristics and Lipid Contents of Desert Date (Balanites aegyptiaca (L.) Del) Kernel and Pulp Oils

Aims: This work is aimed at investigating physicochemical parameters and compositions of fatty acid, phospholipid and sterol of desert date (Balanites aegyptiaca) kernel and pulp. Study Design: Balanites aegyptiaca fruit is one of the oldest feed-stocks in Africa of which little or no attention has been given to it. The plant plays a diverse cultural and traditional role in different societies. Therefore, it is very important to explore more about the chemical composition of the kernel and pulp oils of Balanites aegyptiaca; since it is currently attracting considerable research interest as a result of its diverse beneficial properties. Methodology: The physicochemical parameters, fatty acids, phospholipids and phytosterols of B. aegyptiaca seed and pulp oils have been analyzed and compared with the standards and that of conventional oil for easy assessment of their suitability for nutritional and industrial applications. Results: The results of some physicochemical parameters of kernel and pulp oils were acid value (26.35 and 15.60 mg KOH/g), peroxide value (3.82 and 5.90 meq/kg), saponification value (162.40 and 198.60 mg KOH/g), iodine value (55.20 and 142.50 mg of I/100 g), specific gravity (0.93 and 0.92), kinematic viscosity (2.12 and 1.65 St) and refractive index (1.41 and 1.39), respectively. The most concentrated fatty acids were palmitic acid (14.53%) < linoleic acid (35.65%) < oleic acid (38.27%) for the kernel oil while that of pulp oil were linolenic acid (8.21%) < oleic acid (16.80%) < palmitic acid (32.70%) < linoleic acid (33.56%). Arachidic, behenic, lignoceric and myristic acids were all present in small quantities with none of them recording up to 1.0% in either of the samples. Caprylic, capric and lauric acids were determined but not detected in both oils. The fatty acid composition of kernel and pulp oils contained a healthy mixture of all the types of saturated and unsaturated fatty acids. The value of polyunsaturated/saturated index (P/S) which is associated to the impact on human health was higher in the pulp oil (2.47). Phosphatidylcholine had the highest content in both oils that is 75.99 and 25.88 mg/100 g, respectively. The total values of phytosterols for kernel and pulp oils were 85.00 and 9.87 mg/100 g, respectively. Conclusion: Balanites aegytiaca kernel and pulp oils have the potential to substitute several materials used in manufacturing oil in the chemical and pharmaceutical industries. However, in order to extend usage, these oils should be refined in order to improve the colour and taste.

The male sling for stress urinary incontinence: tips and tricks for success

ABSTRACT Urethral slings are a good treatment option for mild male stress urinary incontinence. There are many different sling options, but herein our group describes our techniques with the Advance® and Virtue® slings. More important than technique, we strongly think that patient selection is paramount to sling success. We only offer slings to patients who have low 24 hour pad weights, high Valsalva leak point pressure, and no history of pelvic radiation. Still, like with any surgery, we recommend that the surgeons implant the device that they are most comfortable with along with their chosen techniques.

Validation of a Novel Endoscopic Retrograde Cholangiopancreatography Cannulation Simulator

Background/Aims@#Endoscopic retrograde cholangiopancreatography (ERCP) requires a unique skill set. Currently, there is no objective methodology to assess and train a professional to perform ERCP. This study aimed to develop and validate a novel ERCP simulator. @*Methods@#The simulator consists of papillae presenting different anatomy and positioned in varied locations. Deep cannulation of the pancreatic duct, followed by the bile duct, was performed. The time allotted was 5 minutes. The content validity indexes (CVIs) for realism, relevance, and representativeness were calculated. Correlation between ERCP experience and simulator score was determined. @*Results@#Twenty-three participants completed the simulation. The CVIs for realism were orientation of duodenoscope to papilla (1.00), angulation of papillotome to achieve cannulation (0.71), and haptic feedback during cannulation (0.80). The CVIs for relevance were use of elevator (1.00), wheels to achieve en face orientation (1.00), and papillotome for selective cannulation (1.00). Regarding CVI for representativeness, the results were as follows: basic cannulation (0.83), papilla locations (0.83), and papilla anatomies (0.80). The novice, intermediate, and experienced groups scored 6.7±8.7, 30.0±16.3, and 74.4±43.9, respectively (p<0.0001). There was a strong correlation between the ERCP experience level and the individual’s simulator score (Pearson value of 0.77, R2 of 0.60). @*Conclusions@#This simulator appears to be realistic, relevant, and representative of ERCP cannulation techniques. Additionally, it is effective at objectively assessing basic ERCP skills by differentiating scores based on clinical experience.

Recent Progress on Branched-Chain Amino Acids in Obesity, Diabetes, and Beyond

Branched-chain amino acids (BCAAs) are essential amino acids that are not synthesized in our body; thus, they need to be obtained from food. They have shown to provide many physiological and metabolic benefits such as stimulation of pancreatic insulin secretion, milk production, adipogenesis, and enhanced immune function, among others, mainly mediated by mammalian target of rapamycin (mTOR) signaling pathway. After identified as a reliable marker of obesity and type 2 diabetes in recent years, an increasing number of studies have surfaced implicating BCAAs in the pathophysiology of other diseases such as cancers, cardiovascular diseases, and even neurodegenerative disorders like Alzheimer's disease. Here we discuss the most recent progress and review studies highlighting both correlational and potentially causative role of BCAAs in the development of these disorders. Although we are just beginning to understand the intricate relationships between BCAAs and some of the most prevalent chronic diseases, current findings raise a possibility that they are linked by a similar putative mechanism.

Multicenter Implementation of a New Electronic Medical Record System Leads to Longer Procedure Times and Poor Staff Satisfaction

No abstract available.

Moms' Empowerment Program participation associated with improved physical health among Latinas experiencing intimate partner violence / La participación en la iniciativa Programa de Empoderamiento de las Mamás, se asocia con mejoría de la salud física de las mujeres latinas objeto de violencia de pareja / Participação no Programa de Empoderamento de Mães associada à melhora da saúde física em mulheres latinas que sofrem violência infligida pelo parceiro íntimo

ABSTRACT Objective To evaluate whether participation in the Moms' Empowerment Program (MEP), a 10-week, 10-session intervention designed to provide support and increase access to available community resources for women experiencing intimate partner violence (IPV), enhanced the physical health of participants who self-identified as Latina. Methods Mothers of children ages 4-12 who self-identified as Latina and had experienced IPV within the past two years were recruited at three intervention sites in Michigan, Ohio, and Texas, via community postings and referrals from agencies serving IPV-exposed families. Selected study participants (n = 93) were assigned to one of two groups: Treatment (immediate enrollment in the MEP) or Control (placement on a waitlist with an invitation to participate in the MEP after the 10-week study period). Data were drawn from two structured interviews, one at the time of recruitment for the study (Time One), and one following the intervention or wait period (Time Two). Results After controlling for age, educational attainment, and partner residence (living with a violent partner at the time of the interview), multilevel modeling revealed that improvement in physical health over time was significantly greater among women who participated in the intervention relative to controls. Conclusions These data suggest that enhancing interpersonal connectedness and access to resources positively affects physical health for Latinas experiencing IPV.

RESUMEN Objetivo Evaluar si la participación en la iniciativa Programa de Empoderamiento de las Mamás (PEM), una intervención de 10 sesiones en 10 semanas concebida para prestar apoyo a mujeres autodefinidas como latinas objeto de violencia de pareja y brindarles un mayor acceso a los recursos comunitarios, mejoró la salud física de las participantes. Métodos Participaron en el estudio madres de niños de 4 a 12 años de edad que se autodefinieron como latinas y habían sido objeto de violencia de pareja en los últimos dos años. El reclutamiento de las participantes se hizo en tres sitios donde se realizaba la intervención, en Michigan, Ohio y Texas, por medio de avisos comunitarios y referencias de organismos que atienden a familias expuestas a la violencia de pareja. Las participantes seleccionadas para el estudio (n = 93) se asignaron a uno de los dos grupos siguientes: grupo de tratamiento (se inscribieron de inmediato en el PEM) o grupo de control (se pusieron en una lista de espera y se invitaron a participar en el PEM una vez que transcurrieran las 10 semanas del estudio). Se extrajeron datos de dos entrevistas estructuradas, una realizada al inscribir a las participantes en el estudio (fecha 1) y otra después de la intervención o el período de espera (fecha 2). Resultados Después de controlar la edad, el nivel de escolaridad y el lugar de residencia de la pareja (vivir con una pareja violenta en el momento de la entrevista), el modelo de varios niveles mostró que el mejoramiento de la salud física con el transcurso del tiempo era significativamente mayor en las mujeres que habían participado en la intervención que en las mujeres del grupo de control. Conclusiones Estos datos indican que mejorar la conexión interpersonal y el acceso a los recursos tiene un efecto positivo en la salud física de las mujeres latinas que son objeto de violencia de pareja.

RESUMO Objetivo Avaliar se a participação no Programa de Empoderamento de Mães (PEM), uma intervenção composta por 10 sessões semanais com o objetivo de prestar apoio e melhorar o acesso aos recursos disponíveis na comunidade para mulheres que sofrem violência infligida pelo parceiro íntimo, promove a melhora da saúde física das participantes que se reconhecem como latinas. Métodos Mães de crianças com idade de 4 a 12 anos que se reconhecem como latinas e sofreram violência infligida pelo parceiro íntimo nos dois anos anteriores foram recrutadas em três centros de intervenção em Michigan, Ohio e Texas, por meio de anúncios na comunidade e o encaminhamento de órgãos que fazem o atendimento de famílias expostas à violência infligida pelo parceiro íntimo. As participantes selecionadas para o estudo (n = 93) foram divididas em dois grupos: grupo de tratamento (inscrição imediata no PEM) ou grupo de controle (inscrição na lista de espera com convite para participar do PEM após o período do estudo de 10 semanas). Os dados foram coletados em duas entrevistas estruturadas, uma ao recrutamento para o estudo (momento 1) e a outra após a intervenção ou o período de espera (momento 2). Resultados Após controlar idade, nível de instrução e residência do parceiro (coabitação com o parceiro violento no momento da entrevista), a análise com o modelo de múltiplos níveis demonstrou que houve uma melhora significativamente maior da saúde física ao longo do tempo nas participantes da intervenção em comparação ao grupo de controle. Conclusões Esses resultados indicam que reforçar o senso de conexão interpessoal e melhorar o acesso aos recursos têm um impacto positivo na saúde física de mulheres latinas que sofrem violência infligida pelo parceiro íntimo.

Sexual exploitation and abuse of children online in the Philippines: A review of online news and articles

@#<p style="text-align: justify;"><strong>BACKGROUND:</strong> Advances in information and communication technologies facilitate opportunities for sexual exploitation and abuse of children online (SEACO). Despite the presence of laws against child pornography, trafficking, and cybercrime, the Philippines has become known as a key hub of the billion-dollar global child cybersex industry.</p><p style="text-align: justify;"><b>OBJECTIVE:</b> The objective of this study was to provide insight on SEACO in the Philippines.</p><p style="text-align: justify;"><strong>METHODS:</strong> The methodology consisted of a review of readily available news articles and reports. The search term "Philippines cybersex" was used in Advanced Search in Google News (https://news.google.com.ph) for English language reports from 1995 to 2015 to describe the geographic distribution of media reported cases of SEACO, the profile of victims and offenders, and how offenders and victims became involved in SEACO.</p><p style="text-align: justify;"><b>RESULTS:</b> The review found 55 reports and articles from 2011 to 2015. Cases were reported from 9 regions in the country with victims ranging from 18 months to 17 years in age. Three types of SEACO were identified: live streaming of child sexual abuse, child sexual abuse material (child pornography), and sextortion using minors.</p><p style="text-align: justify;"><strong>CONCLUSION:</strong> Cases of SEACO were found to be present throughout the country. National policies and preventive measures are needed to stem this crime.</p>

Sweat Potassium Decreases with Increased Sweating in Perimenopausal Women.

Objective: To investigate the relationship between rate of sweat production and sweat potassium concentration in premenopausal (PreM), perimenopausal (PeriM) and postmenopausal (PostM) women after a moderate exercise. Study Design: This is a cross-sectional study conducted in May 2012 at the Department of Physiology, University of Benin, Nigeria. Methods: Thirty healthy female volunteers comprising of PreM (aged: 22.5±0.8 yrs, n = 10), PeriM (aged: 46.5±1.1 yrs, n = 10), and PostM (aged: 52.2±0.9 yrs, n=10) participated in the study. Sweat was obtained with a sweat suction apparatus from a 120 cm2 circular area marked on the skin of the face and neck after a 15 min walk on a calibrated treadmill at a speed of 4.2 km/h at 27°C and a relative humidity of 85-95%, followed by measurement of sweat volume (SV) and [K+]. Sweat rate (SR) was determined by dividing the volume of sweat produced by the duration of exercise. Thirst perception (TP) was self-rated using the Visual Analogue Scale (VAS). Results: The PeriM women demonstrated higher SR (ml/min) (P = .01) and SV (ml) (P = .0006) compared to women in the other groups: SR (PeriM = 0.12±0.01; PreM = 0.07±0.02; PostM = 0.06±0.01), and SV (PeriM = 1.7±0.2; PostM = 0.9±0.1). However, they had lower sweat [K+] (mmol/l) (P = .04), compared to their PostM counterparts (PeriM = 19.98±1.5; PostM = 24.90±1.8). Furthermore, sweat [K+] was inversely associated with SR (r = -0.4, P = .02). Also, change in TP (cm) was highest (P = .001) in the PeriM women (PeriM = 2.5±0.2; PreM = 2.1±0.3; PostM = 0.99±0.2). Conclusion: Although excessive sweating can lead to depletion of the body's potassium concentration, the sweat potassium concentration decreases with increased sweating especially in perimenopausal women. This requires further investigation, as it could be an adaptive mechanism inhibiting excessive potassium loss.

Educational strategies for teaching evidence-based practice to undergraduate health students: systematic review / 보건의료교육평가

PURPOSE: The aim of this systematic review was to find best teaching strategies for teaching evidence-based practice (EBP) to undergraduate health students that have been adopted over the last years in healthcare institutions worldwide. METHODS: The authors carried out a systematic, comprehensive bibliographic search using Medline database for the years 2005 to March 2015 (updated in March 2016). Search terms used were chosen from the USNLM Institutes of Health list of MeSH (Medical Subject Headings) and free text key terms were used as well. Selected articles were measured based on the inclusion criteria of this study and initially compared in terms of titles or abstracts. Finally, articles relevant to the subject of this review were retrieved in full text. Critical appraisal was done to determine the effects of strategy of teaching evidence-based medicine (EBM). RESULTS: Twenty articles were included in the review. The majority of the studies sampled medical students (n=13) and only few conducted among nursing (n=2), pharmacy (n=2), physiotherapy/therapy (n=1), dentistry (n=1), or mixed disciplines (n=1) students. Studies evaluated a variety of educational interventions of varying duration, frequency and format (lectures, tutorials, workshops, conferences, journal clubs, and online sessions), or combination of these to teach EBP. We categorized interventions into single interventions covering a workshop, conference, lecture, journal club, or e-learning and multifaceted interventions where a combination of strategies had been assessed. Seven studies reported an overall increase to all EBP domains indicating a higher EBP competence and two studies focused on the searching databases skill. CONCLUSION: Followings were deduced from above analysis: multifaceted approach may be best suited when teaching EBM to health students; the use of technology to promote EBP through mobile devices, simulation, and the web is on the rise; and the duration of the interventions varying form some hours to even months was not related to the students' EBP competence.

Effect of Tiantai No.1 on gene expression profiles in hippocampus of Alzheimer's disease rats by bioinformatic analysis / 中国结合医学杂志

<p><b>OBJECTIVE</b>To study the effect of Tiantai No. 1 [symbol in text] on gene expression profile in hippocampus of Alzheimer's disease (AD) rat, molecular genetic target points of the effect of this drug were defined, its molecular genetic pharmacodynamic mechanism of anti-AD was further explored at molecular gene level, and a scientific basis was provided for its clinical availability and promotion.</p><p><b>METHODS</b>Thirty male Sprague-Dawley rats were divided into three groups with 10 rats per group: sham-operation group, model group and Tiantai No. 1 group. Sterile surgical procedure was applied, the model group with bilateral hippocampal injection of Aβ1-40 was established, and normal saline was used instead of Aβ1-40 in the sham-operation group. One week after the models was made, rats were administered by gastric lavage once every day for three consecutive weeks. The rats of the sham-operation group and the model group were daily fed with purified water by lavage; the rats of the Tiantai No.1 group treated group were administered with Tiantai No.1 by lavage. Total RNAs of hippocampus tissues were extracted with Trizol, the changes of hippocampus gene expression profiles in the above three groups were analyzed by using Affymetrix rat whole genome expression profile microarray.</p><p><b>RESULTS</b>Microarray analysis showed that, compared with the sham-operation group, the hippocampus of the model group had 50 up-regulated genes with significant difference (fold change >2), and 21 down-regulated genes with significant difference (fold change <0.5); compared with the hippocampus of the model group, the hippocampus of the Tiantai No. 1 group was found to have 5 up-regulated genes with significant difference (fold change >2) and 20 down-regulated genes with significant difference (fold change <0.5). The functions of differentially expressed genes of the groups were involved in nervous system's development, neuronic differentiation and function-regulation, cellular growth and differentiation and apoptosis, synaptic occurrence and plasticity, inflammation and immune response, ion channels/transporters, cellular signal transduction, cellular material/energy metabolism and so on.</p><p><b>CONCLUSION</b>Tiantai No. 1 can regulate hippocampal function, and further regulate the brain function of animals in multiple gene target points by a number of ways.</p>

Lack of correlation between vertical distribution and carrier frequency, and preference for open spaces in arboreal katydids that use extreme ultrasound, in Gorgona, Colombia (Orthoptera: Tettigoniidae) / Falta de correlación entre distribución vertical y frecuencia portadora, y preferencia por espacios abiertos en saltamontes arbóreos que utilizan ultrasonido extremo, en Gorgona, Colombia (Orthoptera: Tettigoniidae)

Male Tettigoniidae emit sound to attract conspecific females. The sound is produced by stridulation. During stridulation the forewings open and close, but it is during the closing stroke that the scraper contacts the file teeth to generate the predominant sound components, which are amplified by adjacent wing cells specialized in sound radiation. The sounds usually exceed the sonic boundary and might occur above 40 kHz, reaching extreme ultrasonic frequencies of 150kHz in some species. Here we test the hypothesis that Tettigoniidae species should prefer microhabitats that favour efficient signal transmission, i.e. that there is a relationship of sound frequency with the vertical distribution of the species (from ground to canopy) at Gorgona National Natural Park, Colombia. We sampled 16 trees and four different altitudinal levels between 1 and 20m above the understory vegetation. We placed collecting blankets separated by vertical distances of 5m, and knocked insects down using the technique known as fogging. We found no correlation between vertical distribution and carrier frequency, but there was a preference for open spaces (below the canopy and above the understory) in species using extreme ultrasound. This is the first quantitative description of the vertical distribution in neotropical species of the family Tettigoniidae and its relationship to the calling song frequency. Rev. Biol. Trop. 62 (Suppl. 1): 289-296. Epub 2014 February 01.

Los machos de Tettigoniidae producen sonido frotando las alas anteriores para atraer a sus hembras coespecíficas con fines reproductivos (mecanismo conocido como estridulación). Un borde esclerotizado (o raspador) en el ala derecha es frotado sobre una vena modificada con dientecillos en el ala izquierda. Durante la estridulación las alas abren y cierran, pero es durante el cierre que el raspador contacta los dientecillos de la lima y produce vibraciones que son amplificadas por celdas adyacentes especializadas para radiar sonido. Sonidos que superen los 20 000Hz, se consideran ultrasónicos, en los Tettigoniidae, la mayoría de los cantos superan el umbral sónico y pueden ocurrir a más de 40 000Hz, con casos extremos de hasta 148 000Hz. La esencia de este trabajo es el estudio de esta gama espectacular de frecuencias de la familia Tettigoniidae y su relación con la distribución vertical de los individuos en las especies que habitan en Gorgona. Se muestrearon 16 árboles a cuatro niveles diferentes de altitud entre 1 y ~20m sobre el sotobosque, instalando sabanas colectoras a intervalos de 4-5m. Los insectos se colectaron mediante el método de nebulización térmica. El análisis indica que no hay correlación entre la distribución vertical de las especies y la frecuencia del canto. Sin embargo se observó una preferencia por espacios abiertos en especies que cantan a frecuencias extremas. Este estudio representa la primera descripción cuantitativa de la distribución vertical de Tettigoniidae Neotropicales y su relación con la frecuencia del canto.

Chemical management in fungicide sensivity of Mycosphaerella fijiensis collected from banana fields in México

The chemical management of the black leaf streak disease in banana caused by Mycosphaerella fijiensis (Morelet) requires numerous applications of fungicides per year. However this has led to fungicide resistance in the field. The present study evaluated the activities of six fungicides against the mycelial growth by determination of EC50 values of strains collected from fields with different fungicide management programs: Rustic management (RM) without applications and Intensive management (IM) more than 25 fungicide application/year. Results showed a decreased sensitivity to all fungicides in isolates collected from IM. Means of EC50 values in mg L-1 for RM and IM were: 13.25 ± 18.24 and 51.58 ± 46.14 for azoxystrobin, 81.40 ± 56.50 and 1.8575 ± 2.11 for carbendazim, 1.225 ± 0.945 and 10.01 ± 8.55 for propiconazole, 220 ± 67.66 vs. 368 ± 62.76 for vinclozolin, 9.862 ± 3.24 and 54.5 ± 21.08 for fludioxonil, 49.2125 ± 34.11 and 112.25 ± 51.20 for mancozeb. A molecular analysis for β-tubulin revealed a mutation at codon 198 in these strains having an EC50 greater than 10 mg L-1 for carbendazim. Our data indicate a consistency between fungicide resistance and intensive chemical management in banana fields, however indicative values for resistance were also found in strains collected from rustic fields, suggesting that proximity among fields may be causing a fungus interchange, where rustic fields are breeding grounds for development of resistant strains. Urgent actions are required in order to avoid fungicide resistance in Mexican populations of M. fijiensis due to fungicide management practices.

Ureteral Cannulation as a Complication of Urethral Catheterization

Urinary catheterization is a common procedure, particularly among patients with neurogenic bladder secondary to spinal cord injury. Urethral catheterization is associated with the well-recognized complications of catheter-associated urinary tract infections and limited genitourinary trauma. Unintentional ureteral cannulation represents a rare complication of urethral catheterization and has been previously described in only eight cases within the literature. We describe two cases of aberrant ureteral cannulation involving two patients with quadriplegia. These cases along with prior reports identify the spastic, insensate bladder and altered pelvic sensorium found in upper motor neuron syndromes as major risk factors for ureteral cannulation with a urinary catheter.

Regulation of T lymphocyte subpopulations in specific pathogen free chickens following experimental fowl adenovirus Ⅷ infection

Two-day-old specific pathogen-free (SPF) chickens were divided into two groups. Group I was inoculated orally with fowl adenovirus VIII (FAV-VIII). Group II served as a negative control. Chickens were investigated at various days post-inoculation (dpi) by flow cytometric analysis for changes in T lymphocyte subpopulations in immune system and blood. In the thymus, CD3+ T lymphocytes were increased at 25 dpi, with significant increases in the FAV infected noted at 1, 12, 20dpi (p<0.05). This was accompanied by a corresponding increase of CD4+ and CD8+ T lymphocytes. In the spleen, CD3+ and CD4+ T lymphocytes were increased significantly at 30 dpi (p<0.01) whereas CD8+ and TCR γ δ+ T lymphocytes were decreased at 1 (p<0.05), 30 dpi (p<0.01). An increase of CD3+, CD4+ and CD8+ T lymphocytes was noticed in peripheral blood, and accompanied by a decrease of TCR γ δ+ T lymphocytes. These results demonstrated that infection with FAV-VIII causes significant fluctuations in T lymphocyte subpopulations in thymus, blood and spleen. It can be concluded that an infection with FAV-VIII has profound effects on the immune system, especially on cell mediated immune competency.

Patella dislocation association with drop foot

All limb injuries should be examined with complete documentation of neurovascular assessment as they are often ignored in the busy emergency department setup. This may lead to delay in such diagnosis during the treatment of limb injuries at a follow-up in orthopaedic clinics. Early diagnosis can help orthopaedic team to investigate and start treatment which may help in the recovery of such neurovascular injuries. We report a case of missed neurovascular assessment in the emergency department on a patellar dislocation of a young person leading to foot drop and sensory numbness in the deep peroneal nerve distribution, which improved completely within three and a half months with conservative treatment. This is also the first reported case of foot drop in association with patellar dislocation

La investigación narrativa en la educación física y el deporte: qué es y para qué sirve / Narrative research in physical education and sport: what is it and what is it for

La creciente atención despertada por la investigación narrativa en las ciencias sociales ha tenido poco eco en la educacjón física y las ciencias dei deporte. En este artículo, tratamos de compensar esta situación indagando en el significado de este tipo de ínvestígación y su utílídad para nuestro campo. Con el fin de comprender esta nueva y compleja forma de investigación, primero definimos qué es 'narrativa'. A continuación, presentamos los supuestos básicos y las características esenciales de la investigación narrativa. Por último, planteamos algunas razones por las que puede resultar beneficioso el estudio de las narrativas en ámbitos específicos de la educación física y el deporte. En conclusión, consideramos que la investigación narrativa puede resultar un instrumento útil, aunque problemático, para la teorización e investigacíón en nuestro campo. No obstante, lIamamos la atención sobre el riesgo de hacer ínvestígacíón narrativa simplemente porque es algo nuevo o que está de moda. Los investigadores y profesíonales que deseamos hacer investigación narrativa debemos informamos acerca de sus pros y sus contras, así como realizar elecciones responsables y juiciosas sobre por qué y cuándo hacerla

A crescente preocupação pela pesquisa narrativa em ciências sociais tem tido pouco impacto sobre a educação física e ciências do esporte. Neste artigo, vamos tentar compensar esta situação questionando o significado desta pesquisa e sua utilidade para o nosso campo. Para compreender esta nova e complexa forma de pesquisa, o que primeiro tentamos definir que é "narrativa". Posteriormente, apresentamos as premissas básicas e características principais da investigação narrativa. Por fim, apresentamos algumas razões pelas quais ela pode ser benéfica para o estudo das narrativas em áreas específicas da educação físíca e esporte. Em conclusão, acreditamos que a investigação narrativa pode ser uma ferramenta útil, mas problemática, para a teorização e pesquisa em nosso campo. No entanto, chamamos a atenção para o risco de fazer investigação narrativa, simplesmente porque ela seja nova ou esteja na moda. Os pesquisadores e profissionais que queramos fazer pesquisa narrativa devemos informar-nos sobre os seus prós e contras e fazer escolhas responsáveis e sensatas sobre por que e quando fazer

Narrative inquiry is a burgeoning form of research in social sciences that has received little attention in physical education and sport sciences.ln this article, we seek to balance this situation by offering an understanding of what narrative inquiry can be and can offer to our field. In order to gain some theoretical purchase on a difficult field, we first define narrative. Next, a distillation of guiding assumptions and characteristics are offered. Finally, some reasons as to why narratives may be of benefit for the field of physical education and sport. In conclusion, we consider narrative inquiry as a useful, although problematic, way of theorizing and doing research in the domains of physical education, sport and exercise sciences. It should not, however, be taken up or practiced simply because it is new or fashionable. Informed, principled, and responsible choices must be made by researchers and applied professionals about why and when they might engage with narrative inquiry should they wish to do so

Interactions of complement proteins C1q and factor H with lipid A and Escherichia coli: further evidence that factor H regulates the classical complement pathway

Proteins of the complement system are known to interact with many charged substances. We recently characterized binding of C1q and factor H to immobilized and liposomal anionic phospholipids. Factor H inhibited C1q binding to anionic phospholipids, suggesting a role for factor H in regulating activation of the complement classical pathway by anionic phospholipids. To extend this finding, we examined interactions of C1q and factor H with lipid A, a well-characterized activator of the classical pathway. We report that C1q and factor H both bind to immobilized lipid A, lipid A liposomes and intact Escherichia coli TG1. Factor H competes with C1q for binding to these targets. Furthermore, increasing the factor H: C1q molar ratio in serum diminished C4b fixation, indicating that factor H diminishes classical pathway activation. The recombinant forms of the Cterminal, globular heads of C1q A, B and C chains bound to lipid A and E. coli in a manner qualitatively similar to native C1q, confirming that C1q interacts with these targets via its globular head region. These observations reinforce our proposal that factor H has an additional complement regulatory role of down-regulating classical pathway activation in response to certain targets. This is distinct from its role as an alternative pathway down-regulator. We suggest that under physiological conditions, factor H may serve as a downregulator of bacterially-driven inflammatory responses, thereby fine-tuning and balancing the inflammatory response in infections with Gram-negative bacteria.

Association of Blood and Bone Lead with a Decrease in Renal Function Indices in Patients with Chronic Kidney Disease / 대한신장학회지

PURPOSE: This study assessed the environmental lead exposure in patients with chronic kidney disease (CKD) and the relationship between lead exposure and renal function indices. METHODS: Seventy-one patients with CKD and 40 control subjects without known renal disease were included. Blood lead was measured by atomic absorption spectrophotometry and tibial lead was measured via 109Cd-based K-shell X-ray fluorescence. Blood urea nitrogen (BUN), serum creatinine, urine creatinine and urine N-acetyl-beta glucosaminidase (NAG) were also measured. Blood lead was corrected with hematocrit (female: 35%, male: 42%) to adjust for differences in anemic status of patients compared with control subjects. RESULTS: The mean level of hematocrit-adjusted blood lead was significantly higher in patients with CKD (4.18+/-1.74 microg/dL) compared with that in control subjects (3.00+/-0.92 microg/dL); the mean tibial lead level tended to be higher in patients with CKD (3.38+/-9.93 microg/g) than that in control subjects (1.28+/-7.92 microg/g), but no statistical significance was observed. In a multivariate regression analysis after adjusting for gender, age, and drinking and smoking status, adjusted blood lead was a significant predictor of increases in BUN and serum creatinine, but not of the level of urine NAG or creatinine. In contrast, no significant association between tibial lead and renal indices was observed in the multivariate regression analysis. CONCLUSION: These results suggest that environmental lead exposure may compromise renal function.

Endoplasmic reticulum stress induced by tunicamycin and antagonistic effect of Tiantai No.1 (1) on mesenchymal stem cells / 中国结合医学杂志

<p><b>OBJECTIVE</b>Changes of the internal and external cellular environments can induce calcium homeostasis disorder and unfolded protein aggregation in the endoplasmic reticulum (ER). This ER function disorder is called endoplasmic reticulum stress (ERS). Severe long-term ERS can trigger the ER apoptosis signaling pathway, resulting in cell apoptosis and organism injury. Recent researches revealed that ERS-induced cell death was involved in the neurocyte retrogradation in the progress of neuron degenerative diseases, such as Alzheimer's disease (AD), Parkinson's disease and so on. Therefore, the protection effect of the traditional Chinese drug-Tiantai No. 1 (1) on the ERS injury of AD was investigated at the molecular gene level in this study with a view to explore the gene pharmacodynamic actions and mechanisms of this drug.</p><p><b>METHODS</b>Primarily cultured marrow mesenchymal stem cells (MSCs) of rats were treated by tunicamycin (TM) in order to induce ERS. RT-PCR, fluorescence immunocytochemistry and Western blot techniques were used to determine the mRNA and protein expression levels of the protective stress protein-ER molecular chaperones GRP78 and GRP94 (which would assist cells to resist cellular stress injury), and to determine the mRNA and protein expression levels of apoptosis promoting molecule Caspase-12 on the membrane of the ER, respectively.</p><p><b>RESULTS</b>Protein expression levels of GRP78 and GRP94 were significantly increased in the TM-induced MSCs, and the mRNA level of Caspase-12 was also remarkably increased in the TM-induced MSCs (P<0.05). All these proved that the ERS model was successfully established by TM in MSC. Meanwhile, the mRNA and protein levels of GRP78 and GRP94 were all significantly increased compared with the model group (P<0.05 or P<0.01) after MSCs were treated with Tiantai No.1 while the mRNA and protein expression levels of Caspase-12 were significantly decreased compared with the model group (P<0.05 or P<0.01). This effect showed a dose dependent manner.</p><p><b>CONCLUSION</b>Tiantai No.1 might attenuate the cell apoptosis induced by ERS injury, and thus protect the neurons against AD.</p>

Embryo transfer in angus and brahman recipient cows: effect of two methods of estrus synchronization on induced estrus and pregnancy

Over a 3-year period, 88 Angus and 87 Brahman cows were used as recipients to determine the effects of breedtype and method of estrous synchronization on induced estrus and subsequent pregnancy following embryo transfer. Estrus was synchronized in recipients using either prostaglandin-F2α (PGF; Lutalyse) or progestogen (PRO; Syncro-Mate-B). Recipients were treated (im) with PGF on day -11 (25 mg), 0 (12.5 mg), and 1 (12.5 mg). Recipients were treated with PRO on day -9 (6 mg norgestomet ear implant and 2 mL [im] of 3 mg of norgestomet and 5 mg of estradiol valerate) and the ear implant was removed on day 0. Embryos were randomly transferred to recipients synchronous +24 to -24 h with a 7-d embryo. Both synchronization methods (PGF vs. PRO) were similarly effective in inducing estrus (89.8 vs. 86.7 percent). Percentage of induced estrus was similar between Brahman (91.0 percent) and Angus (85.6 percent). Recipients treated with PGF had a longer (P = 0.001) interval to estrus than PRO treated recipients (77.4 vs. 60.1 h). Estrus response in Angus was earlier (P = 0.043) than in Brahman (65.4 vs. 72.2 h). Neither breed (P = 0.97; Angus 49 percent 35/72 and Brahman 54 percent 38/70) nor estrus synchronization treatment (P = 0.23; PRO 49 percent 35/72 and PGF 54 percent 38/70) affected pregnancy rate. Pregnancy rates in recipients closely synchronized (0 h) was 60.0 percent, within +12 and -12 h was 56.0 percent, and within +24 and -24 h was 51.5 percent. Four-year-old recipients had lower pregnancy rates (34.8 percent) than did 5-year-old (60.9 percent) or greater than 6-year-old (70.0 percent) recipients. These results indicate that PGF, when administered as a split-second dose, is as effective in synchronizing estrus in Angus and Brahman as PRO. Asynchrony of embryo age with recipient stage of cycle up to +24 or -24 h did not significantly affect pregnancy rates.

Durante tres años, vacas receptoras Angus (88) y Brahman (87) se utilizaron para determinar los efectos de raza y método de sincronización sobre las características del celo inducido y la preñez subsecuente a la transferencia embrionaria. El celo fue sincronizado en las receptoras utilizando PGF2α (Lutalyse®) o progesterona (PRO; Syncro-Mate-B). Un grupo de receptoras fue inyectado (im) con PGF los días 11 (25 mg), 0 (12,5 mg), y 1 (12,5 mg). Otro grupo fue tratado con PRO el dia -9 (implante auricular con 6 mg norgestomet e inyectadas (im) con 2 mL con 3 mg de norgestomet y 5 mg de valerato de estradiol), el implante fue removido el día 0. Los embriones fueron transferidos al azar a las receptoras en sincronía +24 a -24 h con embriones de 7-d de edad. Ambos métodos de sincronización (PGF vs. PRO) fueron similares en la inducción del celo (89,8 vs. 86,7 por ciento). El porcentaje de celos inducidos fue similar en Brahman (91,0 por ciento) y Angus (85,6 por ciento). Las receptoras tratadas con PGF presentaron un intervalo al celo más largo (P = 0,001) que aquellas tratadas con PRO (77,4 vs. 60,1 h). El intervalo al estro post-tratamiento fue más corto en la vacas Angus expresaron celo más temprano (P = 0,043) que las Brahman (65,4 vs. 72,2 h). La tasa de preñez no fue afectada por la raza (Angus 49 por ciento 35/72 y Brahman 54 por ciento 38/70; P = 0,97) ni por el tratamiento de sincronización (PRO 49 por ciento 35/72 y PGF 54 por ciento 38/70; P = 0,23). Las tasas de preñez en receptoras de sincronización cercana (0 h) fue del 60,0 por ciento, entre +12 y -12 h fue del 56,0 por ciento, y entre +24 y -24 h fue del 51,5 por ciento. Las receptoras de cuatro años de edad presentaron tasas de preñez menores (34,8 por ciento) que las de cinco (60,9 por ciento) o las de 6 o más años de edad (70,0 por ciento). Los resultados indican que cuando la segunda aplicación de PGF se administra dividida en dos dosis, es tan efectiva para sincronizar...