Three new lignan glucosides from the roots of Scutellaria baicalensis

Three new lignan glucosides, baicalensinosides A-C (1-3), were isolated from the roots of Scutellaria baicalensis. The structural elucidation was achieved by in-depth spectroscopic examinations and qualitative chemical test. Structurally, these compounds belong to the 3,4-dibenzyltetrahydrofuran-type lignan glycoside with a mono-hydroxyl substitution at the 7'-position of benzylidene group on the numbering system of lignans being one of their shared critical features. The anti-osteoporotic activity of the isolated compounds was assessed in an in vitro osteoprotegerin (OPG) transcriptional activity assay using dual luciferase reporter detection. At 10 μmol/L, compounds 1-3 increased the relative activating ratio of OPG transcription to 1.83, 0.84 and 0.98 times that of the control group, respectively.

Chemical constituents of the roots of Macleaya microcarpa and activation efficacy of benzophenanthridine alkaloids for the transcription of xbp1 gene / 药学学报

Ongoing study on the chemical constituents of the roots of Macleaya microcarpa led to the isolation of eight compounds of derivatives of triterpenes and organic acids in addition to some previously identified benzophenanthridines. The eight compounds were identified by spectroscopic methods as well as comparison with literature values as 1-oxo-2, 22 (30)-hopandien-29-oic acid (1), 3-oxo-12-oleanen-30-oic acid (2), 3α-hydroxy-12-oleanen-30-oic acid (3), 3β-hydroxy-12-oleanen-30-oic acid (4), ferulic acid (5), ferulic acid 4-O-β-D-glucoside (6), 3-O-feruloylquinic acid (7), and methyl 3-O-feruloylquinate (8). Of which, 1 is a new triterpenoid of hopanes and 2-8 are isolated from M microcarpa for the first time. In order to discover natural active compounds as potential agents of anti-ulcerative colitis (UC), an in vitro drug high-throughput screening model targeted x-box-binding protein 1 (xbp1) was employed to evaluate the activity of the major chemical constituents of M microcarpa. The result confirmed that two dihydrobenzophenanthridines, dihydrosanguinarine (9) and dihydrochelerythrine (10), showed a certain activity on activating the transcription of xbpl, a transcription factor (TF) associated with the occurrence, development, and potential treatment of UC, with their relative activating ratios being 1.76 and 1.77 times, respectively, as compared with control group.

Structure determination of five trace chemical constituents from roots of Linum usitatissimum / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the trace chemical constituents from the roots of Linum usitatissimum.</p><p><b>METHOD</b>Isolation and purification of the trace constituents were carried out mainly by solvents extraction and macroporous adsorbing resin and silica gel column chromatography. The structures of the isolates were elucidated by extensive spectroscopic analysis,including 1D and 2D NMR, MS, and HRMS.</p><p><b>RESULT</b>Five trace compounds were isolated and identified as 3-methyl-2, 5-pyrrolidinedione-5-oxime (1), hypoxanthine (2), nonanedioic acid-1,9 (3), and the mixture of isoleucine and leucine(4 and 5).</p><p><b>CONCLUSION</b>Compound 1 is a new compound, and compounds 2-5 are isolated from L. usitatissimum for the first time.</p>

Chemical constituents of rhizomes of Zingiber officinale / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the chemical constituents from the rhizomes of Zingiber officinale.</p><p><b>METHOD</b>Isolation and purification of the chemical constituents were carried out on the column chromatography of silica gel and Sephadex LH-20. The structures were elucidated on basis of physicochemical properties and spectral data.</p><p><b>RESULT</b>Ten compounds were isolated and identified as beta-sitosterol palmitate (1), isovanillin (2), glycol monopalmitate (3), hexacosanoic acid 2,3-dihydroxypropyl ester (4), maleimide-5-oxime (5), p-hydroxybenzaldehyde (6), adenine (7), 6-gingerol (8), 6-shogaol (9), and 1-(omega-ferulyloxyceratyl) glycerols (10a-10f).</p><p><b>CONCLUSION</b>Compounds 1-7 and 10a-10e are obtained from Z. officinale for the first time, and compound 10f is a new compound.</p>

Exclusive control substance of radix Stephaniae tetrandrae / 中国中药杂志

<p><b>OBJECTIVE</b>To develop the system for the exclusive control substance of plant drug (CSPD) in traditional Chinese herbal medicines (TCHM), this paper investigated the (CSPD) of Radix Stephaniae Tetrandrae as well as its proton nuclear magnetic resonance (1H-NMR) and high performance liquid chromatography (HPLC) analytical methods for the purpose of original identification and quality control of the crude drug.</p><p><b>METHOD</b>The CSPDs and their 1H-NMR and HPLC profiles of Radix Stephaniae Tetrandrae were obtained by standardized procedure. Chemical components were isolated from the CSPD by silica gel column chromatography. The assignments of the characteristic signals in 1H-NMR and HPLC profiles were achieved on the basis of elucidation of the isolates structures.</p><p><b>RESULT</b>For nine samples from the different sources in this paper, the 1H-NMR and HPLC profiles from eight sources had wonderful reproducibility and characteristics, and the other gave differences compared with the eight samples in the signal strength of the main components. Furthermore, seven compounds were isolated from CSPD and their chemical structures were authenticated by spectral analysis as tetrandrine, fangchinoline, tetrandrine-2'-N-beta-oxide, tetrandrine-2'-N-alpha-oxide, dicentrine, dicentrinone, and adenine, respectively. The 1H-NMR and HPLC profiles of the CSPD of Radix Stephaniae Tetrandrae showed mainly the characteristic signals of the bisbenzylisoquinoline alkaloids isolated in this work.</p><p><b>CONCLUSION</b>The 1H-NMR and HPLC profiles of the CSPD of Radix Stephaniae Tetrandrae exhibit the structures and total composition of the main active constituents in it, and can be used for its original identification and quality evaluation.</p>

Studies on chemical constituents of roots of Linum usitatissimum / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents from the roots of Linum usitatissimum.</p><p><b>METHOD</b>The compounds were isolated and purified by silica gel column chromatography, their structures were elucidated by physico-chemical properties and spectroscopic data.</p><p><b>RESULT</b>Ten compounds were isolated and identified as vanillic acid (1), syringic acid (2), xanthine (3), vitexin (4), isovanillin (5), (E)-3,3'-dimethoxy-4,4'-dihydroxystilbene (6), tachioside (7), beta-sitosterol and stigmasterol (8 and 9) mixture, berberine (10).</p><p><b>CONCLUSION</b>Compounds 1-3, 5-7, 10 were isolated from L. usitatissimum for the first time.</p>

Investigation on the Structure and Immunomodulating Activities of Polysaccharides from the Seeds of Zea Mays / 国际中医中药杂志

Objective To investigate the glycosidic linkage and immunomodulating activities of crude polysaccharides from the seeds of Zea mays. Methods Extraction, isolation and purification of polysaccharides were carried out with boiling-water extraction plus resolving deposit repeatedly. The structure was elucidated on basis of physicochemical properties and spectral data, and the biological activities were evaluated by means of Immunopharmacological examination. Results The structure of polysaccharides from the seeds of Z. mays exhibited identical structure with rice bran polysaccharides, i.e., a kind of glucan withα-1,4 andα-1,6 glucosidic bonds as the main frame. Conclusion Polysaccharides was obtained from the seeds of Z. mays for the first time, and it showed significant immunomodulating activity in mice.

Studies on Chemical Constituents of Bridelia tomentosa leaf / 国际中医中药杂志

Objective To studythe chemical constituents of Bridelia tomentosa leaf.Methods The constituents of B.tomentosa werg isolated and purified by silica gel,their structures were elucidated by physico-chemical properties and spearoscopic analysis.Results Seven compounds were isolated from B.tomentosa.They were identified as tricin(1),cappariloside A(2),epifriedelanol(3),friedelin(4),tamarixetin(5),β-sitosterol(6),and stigrnasterol(7).Conclusion Compound 1 and 2 were obtained from this plant for the first time.