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Objective To observe the effects of Yougui Pills (YP) intervention on SREBP pathway related factors of sterol regulatory element binding protein-1c ( SREBP-1c) , cholesteryl ester transfer protein ( CETP) and fatty acid synthase ( FAS) protein and gene expression in kidney yang deficiency hyperlipidemia rat model. Methods Sixty SD rats were divided into normal control group, model group (in the dosage of 2.43 g/kg), and YP group. Rat model of kidney yang deficiency hyperlipidemia was induced with intramuscular injection of hydrocortisone. Normal control group and model group were given normal saline, and YP group was given YP suspension intragastrically. After treatment, the hepatic pathomorphology of rats in the three groups was examined, and the contents of serum lipids were examined. Hepatic SREBP-1c, CETP and FAS protein expression was detected by Western-blotting method, and their mRNA expression was detected by real-time polymerase chain reaction ( RT-PCR) . Results Compared with the normal control group, serum triglyceride ( TG ) , total cholesterol ( TC ) , low-density lipoprotein cholesterol ( LDL-C ) contents were significantly increased (P<0.01), high-density lipoprotein cholesterol (HDL-C) was significantly decreased (P<0.01), and hepatic pathomorphological changes of hyperlipoidemia were obvious in the model group. Compared with the model group, TG, TC, LDL-C contents were significantly decreased ( P<0.01) , and HDL-C was significantly increased (P<0.01) in YP group. Model group had higher SREBP-1c, CETP and FAS mRNA and protein expression than the normal control group ( P<0.05) , while YP group had lower SREBP-1c, CETP and FAS mRNA and protein expression than the model group ( P<0.05) . Conclusion YP can decrease the blood lipid levels probably by down-regulating the expression levels of SREBP-1c, CETP and FAS gene and protein related to the the SREBP pathway in rats of kidney yang deficiency hyperlipidemia induced by intramuscular injection of hydrocortisone.
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Objective By extra-body imitation release test with prolongation,medicines action on sustainedreleased injection of hydro-cortisone microeapsule was understood. Methods Making use of HPLC method was inspected to result of medicinal lower and die, solution. At the same time, the disaolvent course of microcapsule wall was observed by microscope and electron microscope. Results The release test at beginning 12 - 24 hours with rdease drugs measure was very lower-degree,along with the time more prolong in mierocapsule substance to contact of PBS solution(24～48h) was began disintegrate. The rdease of medicinal concentration also accompany with rising, the rising different concentration PBS solution from starting up to the highest as to general need 3～5 days and keep releasing for several days. Conclusion Sustained-released injection of hydrocortisone mierocapsule can be effectual action of prolongation released time in hind hydrocortisone.
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Objective To discuss the opportunity of operation and selective in the early stage of treatment method. Methods To analyze the clinical data of 31 cases of patient traumatic floating knee since June 1999 to Au-gust 2006 at my hospital. The results of operative treatment and clinical condition were reviewed. Results In this group there were wound infection 5 cases,amputation 1 case,two stage plant the bone technique and inner fiction 2 cases. After following up patients 1 to 3 years(average 25 months). All of the patients were recovered. According to kerstin at working out standard the limbs function of floating knee damage was evaluated so as to obtain the good percentage of 87 % of the knee joint function. Conclusion A.s usually, the complication of multiple organs injury in floating knee damage was most likely to occur if there had been position priority disposed of important internal organs damage and shock strives in 24 hours properly. The fixed thighbone and shinbone fracture with the emergency dis-posal of important blood vessel damage can reduce the amputation rate.
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Objective To discuss the early stage treatment of reduction and the curative effect in coxa dislo- cation with acetabular fracture. Methods According to the different type of coxa dislocation with aeetsbular frac- ture,22 patients were given the distinctive operation and drawn reduction. As to 22 cases combination with acetabu- lar fracture and basis of difference type of the fracture were selected the method of suitable operative entrance or in- ner fixation. In this gronp,22 patients were followed up about 6 month to 3 years(average 15.3 months) after opera- tion. According to the standard of America orthopedic surgery study were commented on curative effect. Results Excellent 15 cases,well 4 casea,pass 2 cases,bad 1 case. Total fine rate were 86% .Conclusion Each kinds type of the hip dislocation cases should needed emergency reduction. The operation treatments were selected first method to settlement the bone shatter displacement in acetabular fracture or unsuitable joint and unsteady joint.
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Objective To evaluate epicardial fat thickness change in coronary artery disease patients by using echocardiography.Methods 36 patients with coronary artery disease convinced by coronary angiography and 36 volunteer underwent transthoracic echocardiographic examination.Besides conventional parameters of heart,epicardial fat thickness on right ventricle were measured.Results Subjects with coronary artery disease showed higher epicardial fat thickness than volunteer subjects with predominant peripheral fat distribution:(9.97?2.88)mm vs (4.34?1.98)mm(P=0.005) and (7.19?2.74)mm vs (3.43?1.64)mm(P=0.004) in men and women,respectively.Conclusion Transthoracic echocardiography provides a new convient method for evaluating epicardial fat thickness prediction in coronary artery disease.
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[Objective] To investigate the renal histological parameters in SD rats and beagles and to supply evidence for renal toxicological and pathological diagnosis. [Methods] Forty SD adult rats and 20 adult beagles (half males and half females) , were respectively executed at 12 weeks and 12 months old. Then, kidney tissue was fixed in 10% neutral formalin, embedded in paraffin, made into slices at 5?m and stained by HE. The larger diameter and shorter diameter of glomerulus in the renal cortex and outer stripe of rats and beagles were determined by Spot Advanced image analysis software. The differences of the above parameters appearing in different species and genders were compared. [Results] The larger diameter was about 96 ?m, and the shorter diameter 76 ?m in the renal cortex of the beagles, and the larger was about 120?m and the shorter 94 ?m in the outer stripe of the beagles. The differences of the larger and shorter diameters were insignificant between the male and female beagles, but the larger and shorter diameters in the outer stripe were longer than those in the cortex. The larger diameter and shorter diameter were about 87 ?m and 64 ?m respectively in the renal cortex of SD rats, and the larger was about 88 ?m and the shorter 64 ?m in the outer stripe of SD rats. The differences of the larger and shorter diameters were insignificant between the male and female beagles, as well as in the outer stripe and the cortex. [Conclusion] The nephron in the outer stripe is larger than that in the cortex of beagles but no difference of the nephron exists in SD rats. The nephron in beagles is larger than that in SD rats, and there is no difference in the nephron between male and female beagles and SD rats.
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Objective To investigate the parameters of liver tissue in SD rats and beagle dogs and to supply evidence for toxicologic pathological evaluation.Methods Forty SD rats and twenty beagle dogs,male in half,were used for the observation.SD rats were sacrificed at the age of 12 weeks and the beagles at the age of 12 months.After complete necropsy examination,all livers were fixed in 10 % neutral buffered formalin solution,embedded in paraffin,sliced at a thickness of 5 ? m and stained with hematoxylin and eosin(HE).The distance of central vein and portal area,and that of central veins in the neighbored lobules were measured by means of an image analyzer Spot Advanced.The difference of the above distance between beagles and rats,and that between male and female were compared.Results The distance of central veins in the neighbored lobules was about 883 ? m in the beagles and about 496 ? m in SD rats,and the distance between lobule center and portal area was about 511 ? m in beagle dogs and about 410 ? m in SD rats.There was no difference between the male and the female.Conclusion The distance of central veins in the neighbored lobules in beagle dogs is longer than that in SD rats,so does the distance between lobule center and portal area.The sex of the animal has no influnce on the size of the lobules.