ABSTRACT
Objective To discuss the effect of body quality management in pregnant women on the growth of body mass and the outcome of pregnancy .Methods 180 pregnant women were randomly divided into observation group and control group .The control group was given routine obstetric medical examination , the observation group received pregnancy body quality management of nursing mode .The nursing effect was compared in two groups .Results In observation group,the cesarean section rate was 23.33％,1 case of gestational hypertension ,2 cases of gestational diabetes , 2 cases of fetal development and 1 case of postpartum hemorrhage .In control group , the cesarean delivery rate was 53.33％,7 cases of gestational hypertension ,10 cases of gestational diabetes ,9 cases of fetal growth restric-tion and 7 cases of postpartum hemorrhage ,the differences between the two groups were statistically significant (χ2 =17.132,4.709,5.714,4.744,4.709,all P<0.05).The observation group did not appear macrosomia ,low quality and neonatal asphyxia , fetal intrauterine distress in 1 case, the incidence rates were lower than that of the control group,the differences between the two groups were statistically significant (χ2 =6.206,7.283,5.142,4.709,all P<0.05).Conclusion During pregnancy,the application of quality management in pregnant women can reduce the rate of caesarean section and the complications of pregnancy ,and optimize the condition of the newborn .
ABSTRACT
Objective To observe the effects of betulinic acid (BA) on proliferation of human hepatoma stem cell;To discuss its anti-cancer mechanism from the aspects of cell cycle and cell apoptosis. Methods HepG2 stem cells were cultivated in vitro and testified the self-renewal capacity. The effects of BA in concentration of 40, 20, 10, 5, 2.5, 1.25μmol/L on the cell vitality of cultured human liver cancer stem cells for 24 and 48 hours were measured with CCK-8 method. The human hepatoma stem cell line HepG2 was administrated by BA at concentrations of 40, 20, 10, 5μmol/L for 48 hours, and cell cycle and apoptosis rate were measured by flow cytometry. Results BA could inhibit HepG2 stem cell proliferation obviously with dose-effect relationship. BA influenced cell cycle, and induced tumor stem cell apoptosis. 40μmol/L BA blocked cell cycle in S phase, and cell apoptosis rate reached 10.86%. Conclusion BA has obvious inhibitory effects on proliferation of HepG2 liver cancer stem cell, which probably plays a part in anti-cancer by influencing cell cycle and inducing cell apoptosis.
ABSTRACT
Liver cancer stem cell plays an important role in hepatocarcinoma occurrence and metastasis.Presently, the two theories about the source of liver cancer stem cells are mature hepatocytes' dedifferentiation and hepatic stem cells' blocked differentiation.Side population cell sorting and different surface antigen labeling are general sorting methods of hepatocarcinoma stem cells.Targeting the liver cancer stem cells population provides an effective way to the treatments of liver neoplasms.