ABSTRACT
Background: Streptokinase, the most well-known fibrinolytic drug, has long been used in the treatment of myocardial infarction. This enzyme is produced from beta-hemolytic bacteria. In this study, the Streptokinase enzyme was extracted from Streptococcus agalactia strain with ATCC12386, isolated from the throat.
Materials and methods: After cultivating the bacteria in the Blood agar medium and evaluating their aggressive activity, the bacteria were transferred to the BHI broth medium and after incubation at 37 ° C for 24 hours, centrifuge was carried out at 4 ° C for 10000 g for 30 minutes. The contents of the tubes were separated by 0.45 micron acetate cellulose filter and the filters were considered as crude enzymes until fibrinolysis activity and non-toxicity test were performed
Results: Among the extracted enzymes, the enzymes of the shamrock 2, 6, 10, 13, 14, 29 and 27 had fibrinolytic activity higher than 30 percent and extraction enzyme from the standard bacteria had less fibrinolysis activity. The toxicity of the extracellular enzyme was lower than that of other samples in 24 hours and 48 hours
Conclusion: Extracting enzymes have a fibrinolysis effect, and this effect is higher in the bacteria isolated from the throat than the standard bacteria. The toxicity of the enzymes varies with time and concentration. It decreases with decreasing toxicity and increases with increasing time. And it can be described as a new, low-risk, and natural remedy for the elimination of blood clot sound