ABSTRACT
A glucosidase inhibition assay has been used to evaluate extracts from higher plants. An enzyme inhibition of fifty percent or more correlated with the observed cytotoxicity of the extracts
Subject(s)
beta-Glucosidase/antagonists & inhibitors , Plant Extracts/pharmacology , Drug Evaluation, Preclinical/methods , Antiviral Agents/pharmacology , Cell Death/drug effects , HIV/drug effects , Lymphocytes/drug effects , Plant Extracts/toxicity , Puerto RicoABSTRACT
Extracts from higher plants were evaluated for inhibitory activity against avian myeloblastosis virus reverse transcriptase. The assay may serve as a useful prescreen for potential anti-HIV bioactives
Subject(s)
Antiviral Agents/pharmacology , Plant Extracts/pharmacology , RNA-Directed DNA Polymerase/antagonists & inhibitors , Drug Evaluation, Preclinical/methods , HIV/drug effects , Puerto Rico , Avian Myeloblastosis Virus/enzymologyABSTRACT
Four bacteria-derived immunopotentiators were tested for their protective effect on a P-388 mouse lymphocytic leukemia model. The microbial test products were prepared from the following bacterial strains: ATCC 35983 Staphylococcus epidermidis isolated from a patient with IV catheter; ATCC 31874, a patented strain listed as Staphylococcus epidermidis isolated from the urine of a cancer patient; ATCC 25615 Staphylococcus hominis obtained from a child with lymphocytic leukemia, and ATCC 25614 Staphylococcus warneri, an isolate from a patient with adenocarcinoma of the breast. A limited degree of protection and prolongation in survival time was observed in the animal group treated with the bacterial strain ATCC 31874