Real time and accelerated stability studies of tetanus toxoid manufactured in public sector facilities of Pakistan

Tetanus is an acute illness represented by comprehensive increased inflexibility and spastic spasms of skeletal muscles. The poor quality tetanus toxoid vaccine can raise the prevalence of neonatal tetanus. WHO has taken numerous steps to assist national regulatory authorities and vaccine manufacturers to ensure its quality and efficacy. It has formulated international principles for stability evaluation of each vaccine, which are available in the form of recommendations and guidelines. The aim of present study was to ensure the stability of tetanus vaccines produced by National Institute of Health, Islamabad, Pakistan by employing standardized methods to ensure constancy of tetanus toxoid at elevated temperature, if during storage/transportation cold chain may not be maintained in hot weather. A total of three batches filled during full-scale production were tested. All Stability studies determination were performed on final products stored at 2-8 Degree C and elevated temperatures in conformance with the ICH Guideline of Stability Testing of Biological Products. These studies gave comparison between real time shelf-life stability and accelerated stability studies. The findings indicate long-term thermo stability and prove that this tetanus vaccine can remain efficient under setting of routine use when suggested measures for storage and handling are followed in true spirit

Stability of oral polio vaccine at different temperatures

The stability of four samples of oral polio vaccine received from different laboratories stored at -20°C, 5°C, 25°C, 31°C and 37°C for different intervals of time was studied. The virus titration were performed by the standard method in tissue culture microtitre plates. There was a little loss in virus titre when samples were kept at -20°C and 5°C for 8 days and 25°C for four days, where as the samples exposed to 25°C for 30 days and 31°C for 12 days showed almost 50% loss in virus titre. The sample subjected to 10 cycles of freezing and thawing did not show any loss in virus titre. Oral polio vaccine stabilized with magnesium chloride or sucrose is quite a stable vaccine and maintenance of a proper cold chain is recommended for the delivery of a potent vaccine in countries with high ambient temperature