ABSTRACT
Objectives: Obesity increases the risk of numerous chronic diseases. Obesity is classified clinically using body mass index [BMI], waist-to-hip ratio, and body fat percentage. The lipoprotein lipase [LPL] gene has been linked to lipoprotein metabolism and obesity. We performed a case-control study to determine the association between LPL gene polymorphisms and obesity-associated phenotypes such as insulin resistance [IR]
Methods: We examined the different LPL gene variants for association in 642 individuals segregated by BMI and IR. Genotyping of the LPL gene -93 and -53 promoter gene polymorphisms were analyzed using polymerase chain reaction-restriction fragment length polymorphism
Results: A substantial association was observed for -93 gene polymorphism of the LPL gene with obesity, while -53 promoter gene polymorphism showed association with IR
Conclusions: We found a significant association between -93 and -53 promoter gene polymorphisms of the LPL gene with obesity and associated phenotypes in the studied population
ABSTRACT
Background: Obesity is a very common disorder resulting from an imbalance between food intake and energy expenditure, and it has a substantial impact on the development of chronic diseases. The aim of this study was to examine the association of INSIG2 [rs7566605] gene polymorphism with obesity and obesity associated phenotypes in North Indian subjects
Methods: The variants were investigated for association in 642 obese and non-obese individuals. The genotyping of INSIG2 [rs7566605] single nucleotide polymorphism was analyzed by the TaqMan allelic discrimination protocol
Results: A significant association was observed for INSIG2 [rs7566605] single nucleotide polymorphism with obesity and obesity-related phenotypes. Furthermore, a significant relationship was found between the rs7566605 and insulin, homeostasis model of assessment-insulin resistance, the percentage of body fat, fat mass, leptin, and adiponectin
Conclusion: The present study observed significant association between INSIG2 [rs7566605] single nucleotide polymorphism and obesity, as well as obesity associated phenotypes in North Indian population
Subject(s)
Humans , Male , Female , Adult , Membrane Proteins/genetics , Intracellular Signaling Peptides and Proteins/genetics , Polymorphism, Genetic , Phenotype , Polymorphism, Single Nucleotide , Body Mass Index , Insulin , Insulin ResistanceABSTRACT
Objectives: Obesity is a common disorder that has a significant impact on morbidity and mortality. Twin and adoption studies support the genetic influence on variation of obesity, and the estimates of the heritability of body mass index [BMI] is significantly high [30 to 70%]. Variants in the fat mass and obesity-associated [FTO] gene have been associated with obesity and obesity-related phenotypes in different populations. The aim of this study was to examine the association of FTO rs9939609 with obesity and related phenotypes in North Indian subjects
Methods: Gene variants were investigated for association with obesity in 309 obese and 333 non-obese patients. Genotyping of the FTO rs9939609 single nucleotide polymorphism [SNP] was analyzed using Restriction Fragment Length Polymorphism Analysis of PCR-Amplified Fragments. We also measured participants fasting glucose and insulin levels, lipid profile, percentage body fat, fat mass and fat free mass
Results: Waist to hip ratio, systolic blood pressure, diastolic blood pressure, percentage body fat, fat mass, insulin concentration, and homeostasis model assessment index [HOMA-Index] showed a significant difference between the study groups. Significant associations were found for FTO rs9939609 SNP with obesity and obesity-related phenotypes. The significant associations were observed between the rs9939609 SNP and blood pressure, fat mass, insulin, and HOMA-index under a different model. Conclusion: This study presents significant association between FTO rs9939609 and obesity defined by BMI and also established the strong association with several measures of obesity in North Indian population
Subject(s)
Humans , Male , Female , Adult , Proteins , Polymorphism, Single Nucleotide , Phenotype , Adipose Tissue , Body Mass Index , Insulin , Blood PressureABSTRACT
Genetic variants of the melanocortin-4 receptor gene [MC4R], agouti related protein [AGRP] and proopiomelanocortin [POMC] are reported to be associated with obesity. Therefore, the aim of this study is to examine MC4R rs17782313, MC4R rs17700633, AGRP rs3412352 and POMCrs1042571 for any association with obesity in North Indian subjects. The variants were investigated for association in 300 individuals with BMI >/= 30 kg/m2 and 300 healthy non-obese individuals BMI <30 kg/m[2]. The genotyping were analyzed by Taqman probes. The statistical analysis was performed by the SPSS software, ver.19 and p /= 30 kg/m2] in North Indian subjects. This study provides the report about the significant association of MC4R [rs17782313] and POMC [rs1042571] with morbid obesity [BMI >/= 30 kg/m2], but MC4R [rs17700633] and AGRP [rs34123523] did not show any association with obesity in the studied North Indian population.