ABSTRACT
The present study was undertaken to determine the correlation between free bilirubin and indirect bilirubin in normal newborn infants with non-hemolytic jaundice, and the possible effect of hemolysis on free bilirubin measurement by the peroxidase method. A prospective study protocol was applied at the Neonatal Unit of the Department of Pediatrics, Escola Paulista de Medicina. Forty-three newborn infants were submitted to measurement of free bilirubin and bilirubin fractions and the extent of hemolysis of the sample was determined. Data were analyzed statistically by the Student t-test. A positive and moderate correlation (r = 0.668; p < 0.01) was detected between free bilirubin and indirect bilirubin. The linear regression equation calculated by the least squares method was as follows: f(x) = 4.562 + 0.382x. The concentration of free bilirubin was inversely proportional to sample hemolysis, the difference being greater at 50 mg/dl hemolysis. Despite these results, however, the use of this correlation is delicate due to the impossibility of establishing it in individual cases. Also, since the samples may show some degree of hemolysis, this factor should be minimized by appropriate sample collection before free bilirubin measurement
Subject(s)
Humans , Male , Female , Bilirubin/blood , Hemolysis , Jaundice, Neonatal/blood , Horseradish Peroxidase , Indicators and Reagents , Infant, Newborn , Jaundice, Neonatal/epidemiology , Methods , Reference Values , Regression AnalysisABSTRACT
Faz-se uma revisäo da literatura em relaçäo à bilirrubina livre, enfatizando a neurotoxicidade de bilirrubina, os métodos de dosagem da bilirrubina livre e da ligaçäo bilirrubina-albumina. Analisam-se isoladamente vários fatores que influenciam a entrada da bilirrubina no cérebro e o acometimento do neurônio, assim como discutem-se vários métodos de medida da bilirrubina livre e os estudos comparativos entre estes
Subject(s)
Humans , Serum Albumin/metabolism , Bilirubin/blood , Jaundice, Neonatal/etiology , ChemistryABSTRACT
amento. Encontrou-se um porcentual de curas