ABSTRACT
Pancreatic adenocarcinoma is important in oncology because of its high mortality rate. Deaths may be avoided if an early diagnosis could be achieved. Several types of tumors overexpress gastrin-releasing peptide receptors (GRPr), including pancreatic cancer cells. Thus, a radiolabeled peptide derivative of gastrin-releasing peptide (GRP) may be useful as a specific imaging probe. The purpose of the present study was to evaluate the feasibility of using99mTc-HYNIC-βAla-Bombesin(7-14)as an imaging probe for Capan-1 pancreatic adenocarcinoma. Xenographic pancreatic tumor was developed in nude mice and characterized by histopathological analysis. Biodistribution studies and scintigraphic images were carried out in tumor-bearing nude mice. The two methods showed higher uptake by pancreatic tumor when compared to muscle (used as control), and the tumor-to-muscle ratio indicated that99mTc-HYNIC-βAla-Bombesin(7-14)uptake was four-fold higher in tumor cells than in other tissues. Scintigraphic images also showed a clear signal at the tumor site. The present data indicate that99mTc-HYNIC-βAla-Bombesin(7-14)may be useful for the detection of pancreatic adenocarcinoma.
Subject(s)
Animals , Humans , Male , Adenocarcinoma , Bombesin/analogs & derivatives , Organotechnetium Compounds/pharmacokinetics , Pancreatic Neoplasms , Adenocarcinoma/pathology , Bombesin/pharmacokinetics , Cell Line, Tumor , Gastrin-Releasing Peptide/analogs & derivatives , Heterografts/pathology , Heterografts , Mice, Nude , Muscles , Pancreatic Neoplasms/pathology , Peptide Fragments/pharmacokineticsABSTRACT
This study describes infection of microsporidia in the natural fauna of small wildlife animals in an area of deforestation for a water reservoir construction in the state of São Paulo, Brazil. It was focused on marsupials, small rodents, frogs, primates, bats, and others mammals taken from this area to access whether they may represent environmental sources of these zoonoses. From all captured animals, microsporidia spores were observed in the feces of bats, small rodents, and marsupials. This study emphasises the importance of wild animals, particularly small mammals as potential sources of microsporidia to human and animal populations from deforested areas.
Subject(s)
Animals , Feces/parasitology , Measures of Disease Occurrence , Microsporidia/isolation & purification , Animals, Wild/parasitology , Brazil/epidemiologyABSTRACT
Avaliou-se a atividade oxidativa sérica da ceruloplasmina como indicadora dos teores sérico e hepático de cobre e compararam-se dois métodos de determinação da ceruloplasmina, pelo parafenileno e pela ortodianisidina. Foram colhidas 56 amostras de soro e de fígado (biópsias hepáticas) de novilhas Nelore para determinação da atividade sérica da ceruloplasmina e da concentração de cobre. As correlações entre a concentração sérica de cobre e a atividade sérica da ceruloplasmina determinadas pelos métodos do parafemileno e da ortodianisidina foram 0,75 e 0,62, respectivamente. As correlações entre a concentração hepática de cobre e a atividade sérica da ceruloplasmina pelos métodos citados foram 0,15 e 0,12. Não foi observada correlação entre os valores séricos e hepáticos de cobre. A correlação entre determinação da atividade sérica de ceruloplasmina, que utilizou o parafenileno como substrato, e o cobre sérico foi maior do que a correlação entre esse mineral e o método que usou a ortodianisidina.