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1.
Mem. Inst. Oswaldo Cruz ; 87(supl.3): 57-68, 1992. ilus
Article in English | LILACS | ID: lil-121076

ABSTRACT

The development of additional methods for detecting and identifuing Babesia and Plasmodium infections may be useful in disease monitoring, management and control efforts. To preliminarily evaluate sunthetic peptide-based serodiagnosis, a hydrophilic sequence (DDESEFDKEK)was selected from published BabR gene of B. bovis. Immunization of rabbits and cattle with the hemocyanin-conjugated peptide elicited antibody responses that specifically detected both P. falciparum and B. bovis antigens by immunofluorescence and Western blots. Using a dot-ELISA with this peptide, antisera from immunized and naturally-infected cattle, and immunized rodents, were specifically detected. Reactivity was weak and correlated with peptide immunization or infection. DNA-based detection using repetitive DNA was species-specific in dot-blot formats for B. bovis DNA, and in both dot-blot and in situ formats for P. falciparum; a streamlined enzymelinked synthetic DNA assay for P. falciparum detected 30 parasites/mm(cúbicos) from patient blood using either colorimetric (2-15 h color development) or chemiluminescent detection (0.5-6-min. exposures). Serodiagnostic and DNA hybridization methods may be complementary in the respective detection of both chronic and acute infections. However, recent improvements in the polymerase chain reaction (PCR) make feasible a more sensitive and uniform approach to the diagnosis of these and other infectious disease complexes, with appropriate primers and processing methods. An analysis of ribosomal DNA genes of Plasmodium and Toxoplasma identified Apicomplexa-conserved sequence regions. Specific and distinctive PCR profiles were obtained for primers spanning the internal transcribed spacer locus for each of several Plasmodium and Babesia species


Subject(s)
Babesiosis/diagnosis , DNA, Ribosomal/immunology , Malaria/diagnosis , Peptides , Serology
2.
Rev. Fac. Cienc. Méd. (Quito) ; 16(3/4): 9-14, ago-dic. 1991. ilus, tab
Article in Spanish | LILACS | ID: lil-134695

ABSTRACT

En el presente trabajo se compara cinco diferentes métodos diagnósticos y la efectividad del tratamiento, en un estudio con 49 pacientes, a los que luego del diagnóstico clínico de la (s) lesión (es), se realizó Raspado y/o Frotis, Prueba Cutánea de Montenegro (PCM), Cultivo, Biopsia e inmunofluorescencia Indirecta (IFA). La sensibilidad de las pruebas, son confrontadas con el diagnóstico clínico y la remisión de las lesiones en un 100 por ciento posterior al tratamiento empleado (Glucantin y Pentostan), a dosis convencionales. Los resultados en cuanto a porcentajes de positividad son para el Raspado y/o Frotis 42,86 por ciento, cultivo 67,35 por ciento, Prueba Cutánea de Montenegro 97,96 por ciento, biopsia 55,10 por ciento e IFA 67,35 por ciento lo que estadisticamente nos demuestra una alta sensibilidad para la Prueba Cutánea de Montenegro.


Subject(s)
Humans , Male , Female , Dermatology , Leishmaniasis, Cutaneous/diagnosis , Leishmaniasis, Cutaneous/therapy , Skin Manifestations
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