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Introduction:The talus is a tarsal bone articulated with the tibia, fibula, navicular, and calcaneus bones to form supratalar, pretalar, and subtalar joints, respectively. A squatting facet is a kind of anomaly that forms on the surfaces where the tibia and talus articulate. The squatting facet states the daily activities and living style of society. Squatting is described as the hyperflexion of the hip and the knee and the movement of hyperdorsiflexion between the leg and the ankle. Materials and Methods: In the present cross‑sectional study, 600 dry adult human tali were taken from the osteology laboratory in the Department of Anatomy, King George’s Medical University, UP, Lucknow. Each talus was examined for the presence of various patterns of articular facets on the neck of the talus and extensions of its trochlear surface. Ethical clearance was obtained from the Institutional Ethics Committee, King George’s Medical University, Lucknow, as reference code: 121 ECMIIA/P3. Results: In our study, we observed that the lateral, medial, and combined squatting faces are 282 (47.64%), 49 (8.28%), and 22 (3.72%), respectively. Lateral, medial, and continuous trochlear extensions are 126 (21.12%), 120 (20.58%), and 31 (5.36%), respectively, in the Indian population. Conclusion: Modifications of the neck of the talus (squatting facets and trochlear extensions) are the result of prolonged squatting positions, which is a common habit of the Indian population, and incidences of these variations can be used as an anthropological marker for racial and regional differentiation of unidentified bones.
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In this era of cutting-edge research and digitalization, artificial intelligence (AI) has rapidly penetrated all subspecialties, including ophthalmology. Managing AI data and analytics is cumbersome, and implementing blockchain technology has made this task less challenging. Blockchain technology is an advanced mechanism with a robust database that allows the unambiguous sharing of widespread information within a business model or network. The data is stored in blocks that are linked together in chains. Since its inception in 2008, blockchain technology has grown over the years, and its novel use in ophthalmology has been less well documented. This section on current ophthalmology discusses the novel use and future of blockchain technology for intraocular lens power calculation and refractive surgery workup, ophthalmic genetics, payment methods, international data documentation, retinal images, global myopia pandemic, virtual pharmacy, and drug compliance and treatment. The authors have also provided valuable insights into various terminologies and definitions used in blockchain technology.
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Purpose: To analyze the demographics and clinical outcomes of posterior chamber phakic intraocular (IOL) implantation for refractive amblyopia in children and adolescents. Methods: A prospective interventional study was performed on children and adolescents with amblyopia at a tertiary eye care center from January 2021 to August 2022. Twenty?three eyes of 21 anisomyopic and isomyopic amblyopia patients operated for posterior chamber phakic IOL (Eyecryl phakic IOL) as a treatment for amblyopia were included in the study. Patient demographics, pre? and postoperative visual acuity, cycloplegic refraction, anterior and posterior segment examination, intraocular pressure, pachymetry, contrast sensitivity, endothelial count, and patient satisfaction scores were evaluated. Patients were followed up at day 1, 6 weeks, 3 months, and 1 year after surgery, and visual outcomes and complications were documented. Results: The mean age of patients was 14.16 ± 3.49 years (range: 10–19 years). The mean intraocular lens power was ? 12.20 diopter spherical (DS) in 23 eyes and ? 2.25 diopter cylindrical (DC) in four patients. The mean unaided distant visual acuity (UDVA) and best?corrected visual acuity (BCVA) were 1.39 ± 0.25 and 0.40 ± 0.21 preoperatively on the log of minimum angle of resolution (logMAR) chart. Postoperatively, the visual acuity improved by 2.6 lines in 3 months period and maintained till 1 year. Postsurgery, contrast sensitivity in the amblyopic eyes significantly improved, and the average endothelial loss recorded was 5.78% at 1 year, which was statistically insignificant. Patient satisfaction score was statistically significant, with 4.736/5 recorded on the Likert scale. Conclusion: Posterior chamber phakic IOL is a safe, effective, and alternative method for treating amblyopia patients who are noncompliant with glasses, contact lenses, and keratorefractive procedures.
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OBJECTIVE@#To develop a qNMR method for quantitative analysis of triacylglycerols in fruit oil of Hippophae rhamnoides (seabuckthorn, SBT) and analyze commercial samples of SBT oils using GC-MS and FTIR.@*METHODS@#SBT fruit oil (IPHRFH) was extracted with hexane and the triglyceride (TAG) was isolated by vacuum liquid chromatography. Six different branded SBT oils purchased from e-commerce suppliers (Amazon) and in-house prepared SBT oil was analyzed by qNMR and fatty acyl composition of TAGs determined by using NMR. In-house oil was also analysed by GC-MS and FTIR spectroscopy.@*RESULTS@#The qNMR results showed that the oil contained 80.3% of triacylglycerol (TAG). The SBT oil TAGs comprised of linolenate 6.6%, palmitoleate/oleate 65.4%, and total saturated fatty acyl chain including palmitate 28% as determined by qNMR. GC-MS analysis revealed that the major acyl functionalities present in the TAG were palmitoleic acid 36.5%, oleic acid 12.9%, palmitic acid 21.2%, and linoleic acid 18%. Of the six commercial samples analyzed, samples from only one supplier (SW) were fruit oil; All others were the seed oils or mix of fruit oil and seed oil. The labels for samples except for the SW did not indicate whether it was fruit oil or seed oil.@*CONCLUSION@#The results suggest that SBT oil should be analyzed by combination of GC-MS, FTIR and qNMR for factual content of free fatty acid or TAGs, which are chemically different in nature and affect the quality of oil. GC-MS showed the content of omega free fatty acids after hydrolysis, while qNMR and FTIR showed the content of TAGs. The major acyl functionalities found in SBT fruit oil TAGs are palmitoleate/palmitate/oleate, while linoleate and linonelate make up a minor fraction. Furthermore, analysis of commercial samples showed discrepancies between label claims and actual content.
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Purpose: The purpose of this study was to compare and analyze the endothelial cell loss during manual small-incision cataract surgery (MSICS) using the viscoelastic-assisted nucleus removal versus basal salt solution plus technique. Methods: This was a prospective randomized trial of 204 patients who underwent MSICS using viscoelastic-assisted nucleus removal (Group 1- OVD) versus basal salt solution plus technique (Group 2- BSS) at a tertiary eye care hospital in North India from January 2018 to 2021. Of these 204 patients, 103 (50.5%) and 101 (49.5%) were allocated to Group 1 and 2, respectively. The parameters assessed were detailed history, demographics, and anterior and posterior segment details. Visual acuity, intraocular pressure (IOP), keratometry, pachymetry, and endothelial cell density were evaluated preoperatively and postoperatively on day 1 and 30. Results: The mean age of the patients was 64.5 � 8.2 years (range 48� years). There were 129 (63.2%) males and 75 (36.8%) females. The mean LogMAR visual acuity for both groups on day 1 (Group 1- 0.3 � 0.1, Group 2- 0.5 � 0.2) and day 30 (Group 1- 0.1 � 0.2, Group 2- 0.1 � 0.1) was statistically significant (P < 0.001), and the mean IOP value showed a statistically significant value (P < 0.009) on day 1 in Group 2 (15.0 � 2.4 mmHg) and on day 30 (P < 0.001) in both the groups (Group 1- 13.6 � 1.8 mmHg, Group 2- 13.5 � 2 mmHg). The horizontal and vertical k values also showed a statistically significant difference on day 1 and day 30 (P < 0.001). The mean percentage change of central corneal thickness (CCT) in Group 1 was 17.7% and in Group 2 was 17.4% on day 1, and it was 1.1% on day 30 in both the groups, which was statistically significant (P < 0.001) compared to preoperative values. The percentage change in endothelial cell density on day 1 was 9% in Group 1 and 4.6% in Group 2, which was statistically significant (P < 0.001). On day 30, it was 9.7% and 4.8%, respectively, which was statistically significant (P < 0.001). Conclusion: Our study highlights statistically significant endothelial cell loss with viscoelastic-assisted nuclear delivery compared to BSS-assisted nuclear delivery during MSICS in a short follow-up of 1 month. The CCT values showed a slight increase, and the keratometry and IOP were unaffected compared to the preoperative parameters in both the groups
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Introduction: Knowledge of morphometry of lip lining help in deciding the best site for choosing graft for its better uptake during several dermal grafting procedures following craniofacial cancers or cosmetic procedures. It also proves useful in dermatopharmacokinetics, in which we monitor the effect of drugs acting on connective tissue by translabial route and lip augmentation surgeries (esthetic surgery) where care is to be given for dermal fillers not to be injected into the muscle core of lip. Materials and Methods: Ten human male cadavers and 10 human female cadavers were procured. The rectangle‑shaped skin specimen (1 cm × 1 cm) through the upper lip was stained with hematoxylin and eosin stain. A total of 40 slides were prepared. Readings were obtained with the help of CATCAM E series HD cameras which was installed in light microscope. Results: The mean value of thickness of skin (epidermis + dermis) of the lip was 664.72 μm among males while 769.20 μm among females. Conclusion: The epithelium of females is marginally thicker than males. Edp: sc (epidermis/stratum corneum) ratio can suggest that giving drugs through translabial route will be easy in females as compared to males in the upper lip as the stratum corneum is the main barrier in drug transfusion and its absorption secondary to epidermis as a whole. The number of rete pegs per field at the dermoepidermal junction was higher in males which ensures more stability of skin of male lips compared to females
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Background & objectives: Serology testing is essential for immunological surveillance in the population. This serosurvey was conducted to ascertain the cumulative population immunity against SARS-CoV-2 among adults in Jammu district and to understand the association of seropositivity with sociodemographic and clinical correlates. Methods: On September 30 and October 1, 2020, a household survey was done in 20 villages/wards chosen from 10 health blocks in district Jammu, India. Demographic, clinical and exposure information was collected from 2000 adults. Serum samples were screened for IgG antibodies using COVID Kavach MERILISA kit. Tests of association were used to identify risk factors associated with IgG positivity. Crude odds ratio with 95 per cent confidence intervals (CIs) was calculated during univariate analysis followed by logistic regression. Results: Overall adjusted seroprevalence for SARS-CoV-2 was 8.8 per cent (95% CI: 8.78-8.82); it varied from 4.1 per cent in Chauki choura to 16.7 per cent Pallanwalla across 10 blocks in the district. Seropositivity was observed to be comparatively higher in 41-50 and 61-70 yr age groups, among males and in rural areas. Fever, sore throat, cough, dyspnoea, myalgias, anosmia, ageusia, fatigue, seizures, history of exposure, medical consultation, hospitalization and missing work showed significant association with seropositivity on univariate analysis. On logistic regression, only sore throat, myalgia and missing work showed significant adjusted odds of IgG positivity. Extrapolation to adult population suggested that exposure to SARS-CoV-2 was 14.4 times higher than reported cases, translating into Infection fatality rate of 0.08 per cent. Interpretation & conclusions: Since a major part of population was immunologically naive, all efforts to contain COVID-19 need to be vigorously followed while these baseline results provide an important yardstick to monitor the trends of COVID-19 and guide locally appropriate control strategies in the region
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Background: Acute pancreatitis is the most terrible of all the calamities that occur in connection with the abdominal viscera. Prediction of severity is an essential step in the management of acute pancreatitis. 50% of mortality can be reduced to 8% by its early recognition. PANC-3 score is widely available test that can be performed quickly, easy to measure with high accuracy in predicting acute pancreatitis.Methods: This cross-sectional study was conducted in the department of general surgery, VMMC and Safdarjung Hospital over 50 patients admitted with acute pancreatitis. After making the clinical diagnosis, PANC -3 score, modified ATLANTA score, APACHE II were done. CRP and CTSI (computed tomography sensitivity index) were calculated and correlated.Results: Mean age was 44.74 years and most common cause was biliary tract pathology. Mortality observed in 5 patients, 11 patients had severe disease. Sensitivity of PANC- 3 was 81.82%, specificity -92.31% with 75% PPV and 94.7% NPV.Conclusions: PANC-3 can be used to predict the severity of pancreatitis as efficiently as Modified ATLANTA classification/APACHE II. It uses only three criteria which are easily done, and available in the basic health care setup. Its interpretation does not need expertise and can be applied at the time of admission which is an advantage when compared to classical scoring systems.
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Subcellular localization prediction of the proteome is one of major goals of large-scale genome or proteomesequencing projects to define the gene functions that could be possible with the help of computationalmodeling techniques. Previously, different methods have been developed for this purpose using multi-labelclassification system and achieved a high level of accuracy. However, during the validation of our blind datasetof plant vacuole proteins, we observed that they have poor performance with accuracy value range from*1.3% to 48.5%. The results showed that the previously developed methods are not very accurate for the plantvacuole protein prediction and thus emphasize the need to develop a more accurate and reliable algorithm. Inthis study, we have developed various compositions as well as PSSM-based models and achieved a highaccuracy than previously developed methods. We have shown that our best model achieved *63% accuracyon blind dataset, which is far better than currently available tools. Furthermore, we have implemented our bestmodels in the form of GUI-based free software called ‘VacPred’ which is compatible with both Linux andWindow platform. This software is freely available for download at www.deepaklab.com/vacpred.
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Background: To ascertain the prevalence of Hepatitis C infection among patients visiting a tertiary care center in Jaipur, Rajasthan.Methods: An observation analytic study was done at a tertiary care center affiliated to Medical College with retrospective analysis of the hospital data of two calendar years. During this period HCV infection screening (anti-HCV) was offered to every suspected patient admitted in hospital and every pregnant women visiting antenatal care clinic.Results: The study prevalence of HCV infection was 0.05% (13/25311). The prevalence was more in female (0.03%) than male (0.02%). The study prevalence of anti-HCV among pregnant female was 0.02% (3/16224). Maximum positive cases (4/13, 30.77% positive cases) were in the age group of 21-30 years (sexually active group) and >50 years age group while minimum positivity was found in children (00 case, 0-20 years age).Conclusions: In this study, prevalence of HCV infection was 0.05%. The study prevalence of HCV among pregnant females was 0.02%. Maximum positive cases (4/13, 30.77% positive cases) were in the age group of >50 years and 21-30 years. This study aids in view to strengthen proper screening for HCV infection to reduce HCV related morbidity and mortality
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Background: Goiter is one of the most visible manifestations of iodine deficiency disorders (IDD) that is caused due to overstimulation of thyroid as an adaptation to Iodine deficiency. IDD is among the major public health threats and important micronutrient deficiencies in developing countries.Methods: This cross-sectional study was conducted among 6-12 years school children in district Chamba of Himachal Pradesh from February to June 2019. The sample size of 336 was calculated and a total of 504 children from government schools were enrolled in the study. The assessment of goitre was done clinically by inspection and palpation of thyroid gland.Results: In this study, 504 school aged children of 6-12 yrs from district Chamba with mean age of 9±1.86 years were included. The prevalence of total goitre rate was 16% with mean age of 8.75±1.37 years. The highest prevalence (%) was observed in age of 10 years (28.4%) followed by 8 years and 9 years (25.9 % and 23.5% respectively).Conclusions: This study showed mild goitre prevalence in school aged children of 6-12 years in district Chamba of Himachal Pradesh. There is dire need of periodic survey to assess the magnitude of IDD in future.
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Ashawagandha is herb used for various kinds of disease especially as a nervine tonic. Considering these facts many scientific studies were carried out and its memory, anti-stress activities were studied in detail. Aims and Objectives: To study the Phytochemical analysis and Pharmacological Study of Ashwagandha (Withania somnifera) varieties and Withania somnifera (L.) Dunal wild purified with milk steam (WSWM) root powder. To study the efficacy of Wild and Cultivated varieties of Ashwagandha on rats through Elevated Plus Maze test and Morris Water Maze (MWM) model. Materials and Methods: The formulations Withania somnifera (L.) Dunal wild (WSW) root powder, Withania somnifera (L.) Dunal Nagori (WSN) root powder, Withania somnifera (L.) Dunal wild purified with milk steam (WSWM) root powder, PG (Wheat powder placebo) were subjected to preliminary phytochemical screening for the detection of various chemical constituents present. Animal experimentation was done on Wistar Albino Rats obtained from the animal house attached and are divided into three groups consisting of 6 rats per group. Nootropic agents are effectively screened using this paradigm in scopolamine-induced dementia. Elevated Plus Maze and Morris Water Maze (MWM) model are based on this phenomenon. Results: By performing phytochemical analysis, Withania somnifera (L.) Dunal Nagori (WSN) showed the presence of alkaloids, carbohydrates, glycosides, phytosterols, saponins, proteins and amino acids. Withania somnifera (L.) Dunal wild purified with milk steam (WSWM) showed the presence of alkaloids, carbohydrates, glycosides, proteins and amino acids and wheat powder placebo (PG) showed the presence of alkaloids, carbohydrates, glycosides, saponins, phenolic compounds, tannins, proteins amino acids and flavonoids. Conclusion: The formulation group 3 (WSWM) showed remarkable reduction in the transfer latency time (in elevated plus maze test) from the acquisition day to the retention day and therefore considered Group 3 is statistically significant. The formulation group 3 (WSWM) showed remarkable reduction in the latency scores in Morris water maze and hence Group 3 (Ashwagandha wild purified with milk steam (WSWM) root powder) is statistically significant.
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Background: Body donation is a noble act towardscontributing to medical education and research.Although anatomists encourage the general populationto donate bodies, the attitude of anatomists towarddonating their bodies is less discussed in the literature.Aim and Objectives: The present study was conductedto obtain the knowledge, attitude and practice ofanatomists towards voluntary body donation. Materialand Methods: The study was conducted among theanatomists of different Medical Colleges of SouthIndia. To assess the knowledge, attitude, and practicesof voluntary body donation, a semi-structuredquestionnaire was designed and distributed to theanatomists. Results: The study involved 102anatomists. The majority 58.3% stated that the primarysource of supply of cadavers was from the bodydonation program. When it came to voluntary bodydonation, only 32.4% of the anatomists were willing todonate their bodies. The facilitating factors forwillingness to donate were contributions to medicaleducation 60.3%, personal satisfaction 7.4%,motivation to the general public 4.4%, andencouragement from a close friend/ colleague 2.9%.The factors that abstained individuals from donatingwere: restriction from family members 30.9%, malhandling of the cadavers 29.4%, and ritual beliefs20.6%. Conclusion: The approach of anatomiststowards donating their bodies is not promising. It maydiscourage the general public from donating theirbodies. Therefore, it is equally important to inspire andencourage the anatomists towards body donation.
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Background@#Surgical treatment of empyema thoracis in patients with chronic kidney disease is challenging, and few studies in the literature have evaluated this issue. In this study, we aim to report the surgical outcomes of empyema and to analyze factors predicting perioperative mortality in patients with chronic kidney disease. @*Methods@#This retrospective study included data from 34 patients with chronic kidney disease (estimated glomerular filtration rate 2) (p=0.03), ESRD (p=0.02), and late referral (>8 weeks) (p<0.001) significantly affected mortality. @*Conclusion@#ESRD, late referral, and poor functional status were poor prognostic factors predicting postoperative mortality. The decision of surgery should be cautiously assessed given the very high risk of perioperative morbidity and mortality in these patients.
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Purpose: The study purpose included dosimetric comparison of cobalt 60 (60Co) and iridium 192 (192Ir) high dose rate (HDR) source used in brachytherapy treatment of cervical cancer. Materials and Methods: Computed tomography (CT) scans for 15 patients of carcinoma of uterine cervix using 3-mm slice thickness were considered for the study The contouring of high-risk clinical target volume (HRCTV), bladder, and rectum on CT images was done as per the GEC ESTRO guidelines with the help of magnetic resonance imaging images in the treatment planning system. All parameters were kept the same for 60Co (3.5 mm active length, 0.5 mm active dia, Bebig) and 192Ir (3.5 mm active length, 0.6 mm active dia, Bebig) HDR source with 2.5-mm step size and dose prescription to Point A. As per the International Commission on Radiation Unit (ICRU)-89 guidelines, the dose–volume parameters such as D50(Gy), D90(Gy), and D98(Gy) for HRCTV and D0.1cc (Gy), D1cc (Gy), D2cc (Gy), and D5cc (Gy) to the bladder and rectum were calculated for both the HDR sources. Results: The difference in dose–volume histogram parameters such as D50,D90,and D98 of HRCTV was 3.19%, 1.13%, and 0.50%, respectively, for the two radioisotopes. The difference in dose values of D0.1cc, D1cc, D2cc, D5cc, and ICRU reference points of bladder was –0.58%, –0.67%, –0.99%, –0.94%, and –1.75%, respectively. On the other hand, dose difference for D0.1cc, D1cc, D2cc, D5cc, and ICRU reference points of rectum was 0.67%, 0.26%, 0.56%, 0.63%, and –0.33%, respectively. Conclusions: The present study results show that all the dose parameters of HRCTV, bladder, and rectum with 60Co were comparable with those of 192Ir HDR source. The isodose distribution is more bulge out for 60Co in cranial-caudal direction compared to that of 192Ir. However, these differences can be reduced by treatment planning optimization techniques. The clinical plan evaluation in each slice and plane is necessary to explore the logistic and financial benefits of miniaturized 60Co source over 192Ir HDR source
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Objective: To identify phytochemicals as NS5B inhibitors against viral NS5B polymerase in silico model. The NS5Bpolymerase is a hepatitis C virus (HCV) protein involved in the HCV replication. HCV infection can cause progressiveliver damage.Material and Methods: Molecular docking method is used to identify binding efficiency between the NS5B(PDB ID: 3UPI) and the ligands (phytochemicals), i.e., Gallic acid, Catechin, Resveratrol, Apigenin, and Silibinin.Molinspiration tool is also used to determine the druglikeness properties of ligands (Lipinski’s rules of five). Thedocking results were compared to the reference ligand, Dasabuvir.Results: The molecular docking study revealed that all phytochemicals were formed complex with the HCV NS5Bpolymerase via hydrogen bonding interactions. The phytochemicals showed good binding efficacy with the dockingscore: gallic acid (−5.47 kcal/mol), catechin (−7.31 kcal/mol), resveratrol (−8.14 kcal/mol), apigenin (−8.75 kcal/mol),and silibinin (−10.75 kcal/mol) compared to the reference drug, Dasabuvir (−11.43 kcal/mol).Conclusion: The docking results suggested that all phytochemicals showed good binding affinity against hepatitisNS5B polymerase which might be due their antiviral properties.
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Background: To ascertain the prevalence of HBV among HIV-infected, treatment- naïve patients visiting a tertiary care centre in Jaipur, Rajasthan, India.Methods: An observation analytic study was done at a tertiary care centre affiliated to medical college with retrospective analysis of the hospital data of 30 calendar months. During this period routine diagnostic screening of HIV infection and HBV infection was offered to every suspected patient admitted in hospital and every pregnant woman visiting antenatal care clinic. Patients with documentary evidence of HIV infection and history of Hepatitis B vaccination in last 30 days are not screened for these infections at our centre. The HIV screening was done as per NACO guidelines. The HBV screening was done using commercially available enzyme linked immunosorbent assay kits (ELISA) for detection of surface antigen (HBsAg).Results: The study prevalence of HIV infection was 0.11% (40/35289). The prevalence was more in male (0.45%) than females (0.06%). HBV was not detected in any of the HIV positive patient in this study. The study prevalence of HIV among pregnant females was 0.05% (10/22026).Conclusions: In this study, prevalence of HIV infection was 0.11%. The study prevalence of HIV among pregnant females was 0.05%. Other than pregnant women, maximum positive cases (13/30, 43.33% positive cases) were in the age group of 25-34 years (sexually active group). No patient had HIV and HBV co-infection.
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Introduction: Pre-eclampsia, a systemic disease unique topregnancy, affects 3–14% of pregnant women. The aim ofthe present study was to evaluate Neutrophil LymphocyteRatio (NLR) as a bio inflammatory marker of pre-eclampsia(PE), a hypertensive disorder of pregnancy characterized byhypertension and proteinuria after 20 weeks of gestation.Material and Methods: This case control study was carriedout over a period of 10 months after informed consent andethical clearance. The study population included 70 pregnantwomen (35 pre-eclampsia cases and 35 normotensive pregnantsubjects as controls). 3 ml venous blood samples were obtainedfrom both the cases and controls. Complete blood count wasdone using semi-automated three part haematology analyserwhich gives the reading of cell counts, NLR was calculatedmanually.Results: The case group (subjects with pre-eclampsia) werefound to have higher neutrophil to lymphocyte ratio (NLR)than that of the control group. This difference was statisticallysignificant (p<0.001). The receiver operating curve (ROC)analysis showed significant diagnostic accuracy of NLR todiscriminate cases and controls (area under the curve [AUC]= 0.73, P < 0.001) at cutoff value of >= 4.86, 68.6% sensitivityand 80% specificity.Conclusion: Unlike many other inflammatory markers, NLRproves to be an inexpensive and readily available biomarkers,obtained from routinely done complete blood counts that maybe useful for prediction and diagnosis of pre-eclampsia.
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Introduction Type 1 diabetes is an autoimmune disorder characterized by lack of insulin production by the ß cells of the pancreas. This lack of insulin causes a variety of systemic effects on the metabolism of the body, one of which is reproductive dysfunction. The present study investigates the effects of diabetes on the male reproductive system of streptozotocin (STZ)-induced diabetic rats. Material and Methods A total of 18 adult male Wistar rats weighing between 250 and 300 g were included in the present study. The animals were divided into normal and diabetic groups. The diabetic group was further subdivided into 2 subgroups with durations of 24 and 48 days. A single dose of STZ (40 mg/kg body weight) was administrated intraperitoneally to the animals of the diabetic group. After the planned duration, the testes and epididymides were dissected, and their gross weight was measured. The tissues were then processed for histological study. Results The gross weight of the testes and epididymides in diabetic rats at 24 and 48 days showed a decrease in comparison to the control. (p < 0.01 for testes and epididymides). Diabetic animals presented a significant decrease in the diameter of the seminiferous tubules compared with the control group (p < 0.01). The epididymides in the diabetic groups showed a considerable reduction in the tubular surface area compared with the control group (p < 0.01). There was also a reduction in the mean diameter, which was measured using the maximum and minimum diameter of the tubules (p < 0.01). Conclusion The present study is an insight into the adverse effects that diabetes can have on the tissue structure of the testes, of the epididymides, and ultimately on the process of spermatogenesis.
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Knowledge of basic elements provides opportunity to make clear vision of structure of any organ. It is well known that accurate values of thickness of epidermis of skin and its variation with body site, age and sex are important in the fields of medical and biological research. The variations in skin reaction to certain stimuli could be due to biological factors such as the epidermal thickness, dermal thickness, distribution of epidermal appendages etc. Methods: Skin was procured from palm of six freshly embalmed human cadavers. Out of these three were males and three were females. Age of the male and female cadavers ranged between 60 to 70years (mean age 67years). Skin samples measuring 1cm (L) X 0.5cm (B) were taken from center of palm. Tissue was preserved in 10% formaldehyde for 48 hours. Fixed tissue specimens were dehydrated through increasing concentrations (30%, 50%, 70%, 90% and absolute) of ethanol. After clearing the tissue in xylene, embedding was done in paraffin wax. 5 µm thick sections were cut using rotary microtome. Whole thickness of tissue was sectioned. Haematoxylin[Harris’s] and Eosin stained section was observed for 3 different fields thus for each cadaver 27 observations were obtained. Results: In females thickness of epidermis(Edp) of were observed 404.43±23.41µm,thickness of stratum corneum (330±32.83µm),layers of stratum spinosum, stratum granulosum were 4 and 3 respectively, number of rete pegs (7.52±0.94), depth of rete pegs (195.78±18.05µm), thickness of papillary dermis and reticular dermis were(135.92±13.230µm) and (386.10±8.24µm) respectively. Whereas In males thickness of epidermis(Edp) of were observed (296.63±107.03µm),thickness of stratum corneum were (112.20±63.92µm),layers of stratum spinosum and stratum granulosum were 2, number of rete pegs 16.15±3.71, depth of rete pegs (104.56±15.28µm), thicknesss of papillary dermis and reticular dermis were(142.63±40.84µm) and (483.27±116.43µm) respectively. Conclusion: Despite of the fact that subjects from both sexes belonged to the same age group, clear and statistically significant histological differences were observed in the skin of palm. Females showed thicker epidermis as well as stratum corneum but thinner dermis in comparison to males. Males had more number of rete pegs but these are shallower than females.