ABSTRACT
In this study we have reported a new, fast and extraction free spectrophotometric procedure for the assessment of caroverine in pharmaceutical raw and tablet dosage forms. In the reported visible spectrophotometric procedure tungstate in Folin-Ciocalteu phenol reagent is reduced in alkaline medium and produces the blue colored chromogen that shows lambdamax at 740nm with the calibration range of 2-28microg/ml. The LOD and LOQ values are 1.15 and 3.81microg/ml respectively. The newly developed analytical procedure is used to determine caroverine in raw material of and commercial tablets dosage forms. The spectrophotometric method represented in this study is simple, rapid and extraction free. It may easily be utilized for the determination of caroverine in pharmaceutical laboratories for quality control and stability studies purpose
ABSTRACT
Objective: To determinate that male fertility influenced by testicular volume in albino rats
Study Design: Experimental
Place and Duration of study: Institute of Basic Medical Sciences, Dow University of Health Sciences, Karachi, 10 months [November 2009 to August 2010]
Methodology: Sixty four adult albino rats were obtained from animal house Jinnah Postgraduate Medical Centre for the study and divided into 2 groups. Group A received injection normal saline 1 cc intraperitoneally [IP] daily for 8 weeks. Group B received lead chloride in a dose of 10 mg/kg body weight IP daily. On the day of completion of treatment the animals were sacrificed testes along with epididymis removed and place in Petri dish. The length, breath and width of testes were measured with help of vernier caliper. The spermatozoa were obtained from cauda epididymis
Results: The mean +/- SEM of volume testes in group A and B after eight week of treatment were 0.77142 +/- 0.04778 cm[3] and 0.11768 +/- 0.01673 cm[3] respectively. The volume of testes of group B was significantly decreased as compare to group B [P = 0.000]. Mean +/- . The mean +/- SEM number of sperm cells million / ml in groups A and B after eight week of treatment was 7.65 +/- 186706.553 and 1.84 +/- 132792.770 respectively. Number of sperms in group B were significantly decreased as compared to group A [P = 0.000]
Conclusion: There was relationship between volume of testes and male fertility
ABSTRACT
A fast, sensitive and extraction free spectrophotometric method for the quantitative determination of citalopram hydrobromide in pharmaceutical raw and tablet formulations has been proposed. The newly proposed method is based on the charge transfer reaction between citalopram as electron donor and chloranil as electron acceptor. The charge transfer complex of citalopram and chloranil shows lamda[max] at 550 nm in methanol. The experimental conditions such as reaction time, temperature, stoichiometry of the colored complex have been optimized. The developed method allows the determination of citalopram hydrobromide over a concentration range of 1-25 micro g/ ml. The proposed method is used to determine the citalopram in tablet dosage forms. The results of proposed method are compared to the official USP method. The newly developed method is accurate, reproducible and easy to perform. It does not require stringent experimental conditions. No interference has been observed for excipients and additives in tablet formulations