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AIM:To investigate the comparison of total antioxidant capacity in the serum of patients with pterygium and control subjects.METHODS:This case-control study was conducted on all persons referred to Ophthalmology Clinic of teaching Hospital of Vali-Asr (peace upon to him) with clinical symptoms of pterygium during the year 2016.The control group was selected among patients referred to the Ophthalmology Clinic of Vaii-Asr (peace without pterygium) that the two groups were matched in terms of age,gender and place of residence.Sixty-six persons [31 people (47％) in patient group and 35 people (53％) in the control group] were enrolled by convenience sampling.Venous blood sample was taken from all patients after the sampling using ferric reducing antioxidant power(FRAP);FRAP-as a quick 10min measurement,the antioxidant power measurement of samples according to the conversion of ferric iron (Fe3+) to ferrous iron (Fe2+) was checked.The collected data ware entered to software SPSS 21 and were analyzed by chi-square and MannWhitney tests at the level of α =0.05.RESULTS:The mean of antioxidant capacity in patients was 842.55 ± 161.46 μ mol/L and antioxidant capacity in healthy controls was 856.77±209.41 μ mol/L (P=0.8).In the comparison of mean serum antioxidant capacity in healthy individuals and in the serum of people with pterygium based on gender the results showed that the antioxidant capacity mean in male control subjects has been 894.05 ± 176.82 μmol/L and in females control 780.01±118.33 μmol/L that the observed difference have been reported statistically significant (P=0.008) but the other comparison according the gender between cases and control does not show any significant difference.CONCLUSION:The results of this study showed that the full level of serum antioxidant capacity in patients has been less than the mean of antioxidant capacity in control subjects,however the observed difference has not been significant.The results of this study were consistent with basic results carried out on the damaging effects of oxidative stress in the pterygium pathogenesis.Recommending diet with minerals and vitamins containing antioxidants may be preventing the onset and progression of pterygium.
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Background and Aim: Jujube [Ziziphus Jujuba Mill.] is one of the medicinal herbs with grows in dry and semi-dry areas in Iran; mainly in the South Khorasan province. The present study aimed at evaluating antioxidant and free radical scavenging capacity in different types of Jujuba
Materials and Methods: Four ecotypes of Jujubes were collected from different parts of the South Khorasan providence [Sarayan, Quaen, Arish, and Boshad]. The collected samples were air dried and then their aqueous extract was prepared in different dilutions. Anti-oxidant and free radical scavenging capacity of the samples were assessed using 2,2-diphenyl-1-picrylhydrazyl [DPPH] and Ferric Reducing Antioxidant Power [FRAP] methods. Their AAPH-induced hemolysis prevention was also analyzed. The total phenolic content of the samples was assessed using Folin-Ciocalteau method
Results: Maximum phenolic content was obtained from Quaen Jujube [1317+/-4.3 equal to mol Gallic acid]
The highest antioxidant capacity by FRAP [1390.1 +/- 65.5mol/L] also belonged to Quaen jujube. The ability of Arish Jujube extracts in scavenging and neutralizing free radical, tested by DPPH, was always higher compared to the other extracts. Results obtained from the effects of different dilutions of Jujube extracts [0- 25 - 5 mg/ml] on hemolysis showed a dose dependent relationship. All the extracts showed dose dependent reducing hemolysis in a specific range of concentrations, induced by 2,2'-azobis [2-amidinopropane] dihydrochloride [AAPH]. There was no significant statistical difference between jujube ecotypes in preventing hemolysis
Conclusion: According to total phenolic content of the Jujobe extracts, its significant antioxidant properties and radical scavenging activities, which was tested through different methods, it can be a potential booster for anti-oxidant capacities
Subject(s)
Antioxidants , Plant Extracts , Phytotherapy , Plants, Medicinal , Hemolysis , Free Radical Scavengers , AmidinesABSTRACT
Background and Aim: Ephedra, Nepeta, and Hymenocrater herbs have long been used in the treatment of many diseases, but their interactions with cellular components, especially nuclear proteins, are still unknown
On the other hand, in the eukaryotic cell nucleus histone proteins play a main role in the packaging of the genetic material as chromatin. The present study aimed at comparing the in-vitro interactions of aqueous extracts of Ephedra, Nepeta, and Hymonocarater with histone proteins
Materials and Methods: Histone proteins type 2A were purchased from Sigma company and aqueous extract of Ephedra, Nepeta and Hymenocrater were prepared in the research laboratory of Birjand University of Medical sciences. Different concentrations of the aqueous extracts were incubated with histone proteins, then analyzed by UV-Spectroscopy and Circular dichroism
Results: Aqueous extract of Nepeta increased maximum absorbance of histone proteins at 210 nm, but the reduction in absorbance was shown at its high concentration. The absorbance of histone proteins also changed in the presence of the aqueous extract of Ephedra and Hymenocrater and it decreased to zero at higher concentration of Ephedra. Circular Dichroism studies demonstrated that the structure of histone proteins changed in the presence of mentioned aqueous extract; the observed effect of the aqueous extract of ephedra was higher than Nepeta and Hymenocrater
Conclusion: The aqueous extracts of ephedra, Nepeta,and Hymenocrated interacted with histone proteins and changed their structure. The effect of ephedra was higher than others
Subject(s)
In Vitro Techniques , Nepeta , Histones , Plant Extracts , Phytotherapy , Plants, MedicinalABSTRACT
Background and Aim: Oxidative stress and atherogenic dyslipidemia are the main complications in type 2 diabetics. The present study aimed at comparing between serum 8-hydroxydeoxyguanosine [8-OHdG] and atherogenic index plasma [ARP] levels in type 2 diabetics and normal people
Materials and Methods: In this study the relevant data of 50 type 2 diabetics ,who had referred to Emam Reza educational hospital and serum samples of random 50 healthy subjects, aged 27-70 yrs, was collected
The levels of 8-OHdG, AIP, and MDA in type II diabetics were measured by means of ELISA and TBARS, respectively. Their lipid profiles and plasma autherogenic indices were assessed through an autoanalyser
Results: The case and control groups were homogenized regarding some demographic components such as age and gender .It was found that serum levels of 8OHdG,MDA, and AIP were significantly higher in the patients compared to the healthy controls ,aged 27-70 yrs [P<0.05, r=0.31]. But, no significant relationship was found between 8OHdG and MDA [P=0.91, r=0.02]
The high rate of lipid peroxidation and DNA oxidation in type II diabetics can reveal outstanding oxidative stress predisposing them to CVDs complications
Conclusion: The lipid peroxidation and DNA oxidation in diabetic patients were indicated high oxidative stress in these patients, which can cause post complications such as cardiovascular disease
Subject(s)
Humans , Adult , Middle Aged , Aged , Oxidative Stress , Dyslipidemias , Diet, Atherogenic , Healthy Volunteers , Case-Control StudiesABSTRACT
Background and Aim: Increasing the intensity of physical activity along with regular consumption of green tea can be effective on energy metabolism, weight, and body fat content. The aim of the present study was to determine the effect of a 10 week. .high intensity interval training supplemented with consuming green tea .on lipid profiles and body composition in overweight women
Materials and Methods: In this quasi-experimental study, 30 overweight women were purposefully and randomly chosen and divided into 3 equal groups. Training group performed training programs including 3 sessions per week at maximum intensity of 85-95% heart rate and the supplement group consumed 3 tablets of green tea [500 mg] daily for 10 weeks High intensity interval training and the supplement group both underwent intervention. Blood samples were collected before and after the intervention in fasting state. Finally, the obtained data was fed into SPSS software [V. 19] and analyzed using paired t test, covariance analysis, one way-variance analysis, and Bonferroni post- hoc tests at the significant level of P<0.05
Results: After the period of high intensity interval training and green tea supplementation triglycerides [P=0.001], LDL [P=0.02], weight [P=0.0001], body mass index [P=0.0001], and body fat percentage [P=0.0001] in all the groups and total cholesterol [P=0.01] decreased, but HDL [P=0.01] increased in high intensity interval training plus supplements and high intensity interval training plus placebo groups. However, these two indicators did not differ significantly in the supplement group [P=0.23 and P=0.06, respectively]. Furthermore, systolic [P= 0.55] and diastolic [P= 0.15] blood pressure and waist-to-hip ratio [P= 0.08] did not change after intervention in all the groups
Conclusion: It was found that consumption of green tea along with performing of high intensity interval training can be effective in improving of cardiovascular risk factors in overweight women
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Intensive and acute exercise trainings may induce oxidative stress, but antioxidant supplements may attenuate its degenerative consequences. The aim of this research was to examine the effect of green tea supplementation on the oxidative stress indices after an intensive resistance training session. 40 non-athletes [without regular physical activity] women were randomly divided into 4 equal [n=10] groups including green tea supplementation, green tea supplementation plus resistance training, resistance training, and control groups. After supplementation period [600 mg/day, 14 days], resistance training and green tea supplementation plus resistance training groups performed an intensive resistance training session at 75-85% of one repetition maximum. The malondialdehyde and total thiol were measured as oxidative stress indices. Data were analyzed by using of repeated measure ANOVA and LSD tests at p<0.05. Results showed that after 14 days of green tea consumption, malondialdehyde significantly decreased in green tea supplementation [p=0.03] and green tea supplementation plus resistance training [p=0.01] groups, while total thiol increased significantly [p=0.01] in two green tea supplementation groups. However, an intensive resistance training session increased malondialdehyde [p=0.01] without any significantly changes in total thiol [p=0.42]. It seems that green tea supplementation can inhibit exercise-induced protein and lipid oxidation in non-athletes women via enhancement of antioxidant defense system of the body
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Background and Aim: Today, it is known that some natural and synthetic compounds possess antioxidant properties and as a result, have gained a lot of significance in food industry. Many of these compounds have got important roles in protecting the liver against destructive factors. The present study is an attempt to survey the antioxidant properties of berberis vulgarris syrup and its liver preserving effects in the rat damaged by carbon tetrachloride
Materials and Methods: In this experimental study, total antioxidant capacity of berberis vulgarris syrup was assessed at first using FRAP method and phenolic compound content was tested through Folin Ciocalteumethod. Then, radicals neutralizing effects of berberis syrup were examined by means of DPPH method. Our subjects, i.e. 66 rats, were randomly divided into 6 equal groups. Then, they were fed with Berberis vulgarris syrup at different doses [1%, 4%, and 20%] for four weeks Finally serum content of aminotransferase [SGPT and SGOT], alkaline phosphatase, and bilirubin were measured. The obtained data was analyzed using variance analysis and Tukey test through SPSS software [V:15]
Results: BerberisVulgarris syrup has high antioxidant properties as indicated by FRAP method. Results obtained from DPPH method showed that DPPH radicals are better controlled by reducing their production to the consumption interval. Folin Ciocalteu test demonstrated that with an increase in the polyphenol combination, through reducing production to consumption time, a significant increase in polyphenol combination occurred. Tetrachloride carbon treatment significantly increased serum level of alanine aminotransferase, and aspartate transferas of the sample [P
Conclusion: As suggested by the results, BerberisVulgarris syrup failed to preserve the liver of the rat against oxidative damages caused by carbon tetrachloride but iteven showed a proxidative effect
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Metabolic syndrome is a major risk factor for coronary artery disease [CAD]. Aim of this study was to determine the prevalence of metabolic syndrome in patients with premature myocardial infarction [before 50 years of age]. In this case-control study, we compared 98 consecutive patients who were hospitalized in Birjand with acute first myocardial infarction before the age of 50 years and 98 age- and sex-matched healthy controls without a history of coronary artery disease. The case and control groups were categorized according to the National Cholesterol Education Program Adult Treatment Panel [NCEP ATP III] metabolic syndrome criteria [presence of >/= 3 of the following: Fasting blood glucose >/= 100 mg/dL, triglyceride [TG] level >/= 150 mg/dL, low high density lipoprotein [HDL; <40 mg/dL in men and <50 mg/dL in women], blood pressure >/= 130/85 mm Hg, and waist circumference >102 cm in men or 88 cm in women]. The data were collected and analyzed by t-test, chi[2], and logistic regression in SPSS software 11.5. Prevalence of metabolic syndrome was significantly higher in cases than in control group [34.7% in cases, 16.3% in controls, P=0.003]. All components of metabolic syndrome except high waist circumstance in the cases group were significantly higher than in control. The most common component of metabolic syndrome was high TG and the least common component was low HDL. We conclude that prevalence of metabolic syndrome in patients with premature myocardial infarction is high; high TG is the most common component of metabolic syndrome
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Myocardial infarction in young age is increasing. Identifying risk factors could be important for health promotion. We studied classic atherosclerotic risk factors in premature myocardial infarction. In this matched case-control study, which was conducted from 2005 to 2007 in Birjand County, the east of Iran, atherosclerotic risk factors [hypertension, family history of coronary artery diseases, obesity, diabetes mellitus, dyslipidemia] of 98 patients affected by acute myocardial infarction aged under 50 years were compared with that of 98 healthy neighborhood controls. Mean levels of cholesterol, triglyceride, low-density lipoprotein, as well as systolic blood pressure and body mass index were significantly higher in cases than in controls. There was a positive association between coronary artery disease at younger age and dyslipidemia OR=2.8 [95% CI: 1.5, 5.2], smoking OR=6.4 [95% CI: 3.0, 13.5], systolic hypertension OR=3.1 [95% CI: 1.5, 6.3], family history of coronary artery diseases OR=10.9 [95% CI: 3.2, 37.9] and diabetes OR=2.5 [95% CI: 1.04, 6.2]. Smoking, systolic hypertension and dyslipidemia were the most common risk factors among patients with premature myocardial infarction
Subject(s)
Humans , Male , Female , Atherosclerosis , Coronary Artery Disease , Case-Control Studies , Risk Factors , Hypertension , Diabetes Mellitus , Obesity , Dyslipidemias , SmokingABSTRACT
Intestinal parasitic infection is one of the major public health problems in developing countries. Children are more vulnerable to these infections. In addition, the prevalence of infection is different among various communities; hence, there is a need for the periodical prevalence evaluation. This study was performed to define the prevalence of intestinal parasitic infection among the students of South Khorasan Province, eastern Iran in 2007. A cross-sectional study was performed on 2169 students aged 6-11 years in six cities of South Khorasan Province in 2007. Three stool specimens were collected from each student. Specimens were examined with direct wet and formalin ethyl acetate method. Data were analyzed with SPSS version 15 software. From a total 2169 students, 47.7% were infected with one or more intestinal parasites. Almost 33.4% were pathogen parasites. The most common parasite was Giardia [28.7%]. The prevalence rate of infection was significantly higher in rural area than that of urban area [P=0.001]. The prevalence of infection was also much more common in those students whose parents were less educated. Prevalence of intestinal parasitic infection in this region is remarkable. Public health education and using healthy water are recommended
Subject(s)
Humans , Male , Female , Child , Cross-Sectional Studies , Giardia , SchoolsABSTRACT
Objective. To determine the relationship of body mass index with serum lipids in elementary students. Methods. This prospective analytic study was conducted among 954 elementary school students (9-11years), selected by multi stage random systematic method from 6 cities and their rural areas from The South Khorasan province (eastern Iran) from September to December 2006. Height and weight was measured and Body mass index was calculated. Total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) were determined. Results. 954 students 9-11 years old were studied. 45.4% were boys. 76.5% were living in the city. 1.8% of students were obese and 3.4% were over weight. There was no significant relation between obesity and overweight with sex, age and the area of residence. There was significant relation between BMI with TC (P= 0.003), TG (P< 0.001) and LDLC (P= 0.04). TG was significantly higher in obese and overweight students than in normal weight students (P< 0.001). TC (0.002) and LDL-C (0.01) were significantly higher in obese students than normal weight students. The prevalence of high TG was significantly higher in obese and overweight students than normal weight students (0.003). There was no significant difference between different kinds of dyslipidemia with area of residence. Conclusion. it is necessary to measure serum lipid profile in obese and overweight children.
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Zinc deficiency is common in developing countries and can affect growth of children. The objective of this study was to determine the prevalence of zinc deficiency and its effect on growth in elementary school children of South Khorasan province. This cross-sectional study was carried out on 908 9-11 year-old school children of South Khorasan province [East Iran] from October to December 2007. The subjects were chosen by multi-stage cluster random sampling method from urban and rural areas. Anthropometric measurements were made following standard techniques. Children were considered Stunting, underweight and wasting as height-for-age and weight-for-age and weight-for-height Z-score below or equal -2 standard deviation using the National Center for Health Statistics [NCHS] reference population. Serum zinc level was measured by atomic absorption spectrometry. Statistical analysis was performed by the SPSS statistical package using t-test, Chi-square and ANOVA. Totally, 474 subjects [52.2%] were girls and 697 subjects [76.8%] resided in city. The prevalence of stunting, underweight and wasting was 13.2%, 6.8% and 5.5%, respectively. The mean value of zinc plasma level was 87.7 [ +/- 32.7] micro g/dl; it was significantly higher in girls and in urban areas. There were no significant differences in mean serum zinc in school children with normal growth and subjects that were stunted, underweight or wasted. The prevalence of zinc deficiency was 28.1% and it was significantly higher in rural areas. The prevalence of zinc-deficiency was significantly higher in stunted school children than non-stunted children. Zinc deficiency is common in elementary school children of South Khorasan province, so searching studies for underlying factors of zinc deficiency and supplementation of zinc, especially in stunted students is recommended
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Humans , Male , Female , Prevalence , Schools , Child , Cross-Sectional Studies , GrowthABSTRACT
The importance of cardiovascular risk factors like hypertension, obesity and dyslipidemia in prediction of later coronary artery disease [CAD] in offspring of high-risk family is well known. This study was performed to compare the level of lipoprotein [a] and apolipoproteins as new risk factors in children and adolescents with and without a family history of premature CAD This case-control study was performed from November 2004 until September 2005. All patients with premature myocardial infarction hospitalized in the coronary care units [CCU] of Vali-e-Asr hospital, who survived and had children between 2-14 years old, were defined as parents of the case group. 86 of them were chosen with simple non-random method. Only one child from each family was selected randomly. The control group consisted of children with nearest age and sex to children of the case group from the neighbors with equivalent socioeconomic status, without a family history of premature myocardial infarction. Subjects had been instructed to fast for 12 to 14 hours. Venous blood was analyzed for lipoprotein [a] and Apolipoprotein A1 and B100. The level of lipoprotein [a] was significantly higher in the case group. There was not a significant difference of lipoprotein levels between the two groups. Measurement of lipoprotein [a] is recommended in screening programs in offspring of high-risk families
Subject(s)
Humans , Male , Female , /etiology , Family , Child , Apolipoproteins , Risk Factors , Hypertension , Obesity , Dyslipidemias , Case-Control Studies , Myocardial InfarctionABSTRACT
The GB virus-C [GBV-C] and Hepatitis G virus [HGV], collectively known as GBV-C/HGV and transmitted through blood transfusion and blood components. A co-infection of HGV and HCV is often seen in patients with hemophilia. The paucity of information about rate of GBV-C infection among hemophilic patients in Iran promoted the current study. This study was performed on 80 hemophilic patients from south Khorassan branch of Iranian hemophilia society in Birjand. All 80 serum samples were tested for hepatitis B surface antigen [HBs-Ag], Anti HCV, Anti HIV, and Anti HTLV-1. All sera positive for HCVAb were retested by recombinant immunoblot assay as a complementary test. Also, Serum HCV-RNA, HCV genotyping and HGV-RNA were detected. The prevalence of HGV-RNA was 5% [4 of 80]. The prevalence of Anti HCV positive was 26.3% [21 of 80] and HCV- RNA was detected in 80% [17 of 21] of these patients. Co infection of HGV with HCV was 5%. HBsAg and Anti HIV were negative in all of our patients. Anti HTLV-1 was detected in one patient [1.25%]. HGV and HCV are prevalent in South Khorassan hemophilic patients. Prevalence of HGV infection is less than HCV but it is more prevalent than HBV, HIV and HTLV-1 infection