ABSTRACT
Background: HCC comes in the sixth rank in all tumors that influence the human body and it is generally connected with interminable liver cirrhosis in 80% of patients. In Egypt, the rate of HCC has expanded pointedly in the last decade. It is considered as a heterogeneous illness including variable types of neoplasms, which includes distinctive profile changes in both mRNA and miRNA expression. One of them is miRNA 483-5P which was found to be up-regulated in hepatocelleular carcinoma
Objectives: To assess the diagnostic and prognostic potential of circulating miRNA 483-5p in HCC in Egyptian patients in comparison to chronic liver disease patients and healthy controls
Methodology: This study was done on 100 HCC patients, 50 chronic liver disease [CLD] due to HCV infection and 50 healthy subjects. Serum alpha fetoprotein [AFP] was measured in all subjects by Chemiluminescence. The relative quantitation of miRNA 483-5p was determined by real-time quantitative Polymerase Chain Reaction [RT-qPCR]
Results: the results revealed an over expression of miRNA-483-5p in HCC group [p < 0.001] compared to both CLD and healthy subjects, while no significant change was detected between CLD and healthy subjects. MiRNA483-5p expression increased significantly with increased number of focal lesions and size of tumor. Receiver Operator of Characteristics [ROC] curve analysis of plasma miRNA-483-5p revealed that, at a cut-off value of 3. 22 [fold expression], the sensitivity and specificity for differentiation of HCC cases were 88.0% and 92.0%, respectively
Conclusion: Circulating miRNA483-5p can be used as an early diagnostic biomarker for detection of HCC