ABSTRACT
Lactoferrin [LF] is a cationic iron -binding protein residing in the specific granules of poly-morph-nuclear neutrophil leucocytes [PMNL] .It is released upon neutrophil activation. Anti-LF antibodies .belong to anti-neutrophil cytoplasmic antibodies [ANCA] It has been described in several autoimmune diseases as classical Rheumatoid arthritis [RA] and Systemic Lupus Erthymatosis [SLE] and has a role in vasculitic changes associated with these diseases. The aim of this study was to assess anti LF antibodies and serum ferritin in different types of Juvenile Rheumatoid Arthritis [JRA] and its relation to disease activity. Serum samples were collected from forty four patients with JRA diagnosed according to Cassidy, et al[11] criteria, 16 of them were pauciarticular type, [mean age 7.2 +/- 2.4 years],16 polyarticular [mean age 6.8 +/- 2.3 years], and 12 systemic onset type [mean age 7 +/- 3 years], in addition to 12 healthy subjects as control group. Clinical assessment of patients and controls was done with stress on fever, skin rash, joint swelling and tenderness and subcutaneous nodules. Laboratory measurement of anti- LF antibodies, serum ferritin, TIBC, ESR, RF, CRP and ANA. was done The results of our study showed high serum levels of both anti-LF antibodies and serum ferritin in all types of JRA patients' in comparison to control group. Anti- LF antibodies showed significant' positive correlation with clinical and laboratory variables of disease activity, including fever, joint swelling, SCN ,ESR, and CRP. Serum feriritin showed statistically significant high level in systemic onset type, and positive correlation with anti-LF antibodies. TIBC showed significant negative correlation with both Anti-LF antibodies and serum ferrtin. Anti-antibodies LF can be used as a marker of activity. Serum ferrtin is Considered as inflammatory marker and has a significant diagnostic value in systemic onset type of JRA.
Subject(s)
Humans , Male , Female , /blood , Biomarkers/bloodABSTRACT
Abstract: Soluble intercellular adhesion molecule -1 [sICAM-1] is ranked as one of the endothelial dysfunction markers and factors predicting diseases of the cardiovascular system. Polycystic ovary syndrome [PCOS] is an extremely prevalent disorder in which altered endothelial function and insulin resistance have been found
The main Objective: To assess serum level of soluble intercellular adhesion molecule-1 [sICAM-1] as a marker of endothelial dysfunction in relation to insulin resistance and serum testosterone in women with PCOS
Subjects and Methods The study assessed 40 women with PCOS [mean age 25.57 + 3.25 year]. That group was further divided into two subgroups: the first with body mass index [BMI] > 25kg/m2 [20 women of mean age 25.6 + 3.5 years] and the second with BMI< 25 kg/m2 [20 women of mean age [25.5 + 30 years]. In the control group there were 30 healthy women [mean age of 24.9 + 3.24 years]. That group again was divided accordingly to BMI in to two subgroup: the first with BMI> 25 kg/m2 [15 women, mean age 25 + 3.1 years] and the second with BMI <25 kg/m [15 women, mean age 24.9 + 3.4 years]
Results: The results demonstrated statistical significant higher mean concentrations of sICAM-1 in women with PCOS as compared to control group and also from the control subgroup with BMI> 25 kg/m2. There was statistically significant positive correlation between sICAM-1 and Insulin resistance [HOMA ]in PCOS and total testosterone level. There was statistically significant correlation between sICAM-1 and BMI in group with PCOS
Conclusion: higher concentration of sICAM-1 in women with PCOS, a significant degree of insulin resistance exist, which clearly is not related to obesity, may confirm the presence of endothelial dysfunctions, a higher risk for cardiovascular diseases in this group. Further study are therefore necessary to identify both the factors that are responsible for the increase in sICAM-1 concentration and the clinical usefulness of routine sICAM-1 measurement in females with PCOS