ABSTRACT
Background@#Pterospermum rubiginosum has been traditionally used by the tribal inhabitants of Southern India for treating bone fractures and as a local anti-inflammatory agent; however, experimental evidence to support this traditional usage is lacking. The present study aimed to investigate the phytochemical characterization,In silico and in vitro anti-inflammatory evaluation, followed by in vivo toxicological screening of P. rubiginosum methanolic bark extract (PRME). @*Results@#The LCMS evaluation revealed the presence of 80 significant peaks; nearly 50 molecules were identified using the LCMS database. In silico analysis showed notable interactions with inducible nitric oxide synthase (iNOS) and interleukin-6 (IL-6). In vitro gene expression study supported the docking results with significant down-regulation of iNOS, IL-6, and IL-10. PRME was administered orally to the SD rats and was found to be non-toxic up to 1000 mg/kg body weight for 14 days. The antioxidant enzymes catalase and sodium dismutase exhibited an increased value in PRMEadministered groups, possibly due to the diverse phytochemical combinations in bark extract. @*Conclusions@#PRME administration significantly downregulated the gene expression of inflammatory markers, such as iNOS, IL-6, and IL-10. The molecular docking analysis of iNOS and IL-6 supports the in vitro study. In vivo toxicological study of PRME in SD rats was found to be non-toxic up to a concentration of 1000 mg/kg body weight for 14 days.
ABSTRACT
The purpose of the study was to determine the effect of early ambulation on maternal outcome among post caesarean mothers. The objectives of the study was to assess the effect of early ambulation on maternal outcome among post caesarean mothers and to ?nd out the association between the maternal outcome among post caesarean mothers with selected demographic variables. This study was based on Roy's adaptation theory. The research design adopted for the study was post-test only control group design. 60 post caesarean mothers who ful?lled the inclusion criteria were selected for the study, out of which 30 post caesarean mothers were in experimental group and 30 post caesarean mothers were in control group. Non-probability purposive sampling technique was used to select the samples. The tool used to assess the effect of early ambulation was post caesarean bio physiological health parameters chart. The study was conducted in General Hospital, Neyyattinkara. The effectiveness of early ambulation was assessed by comparing the maternal outcome of post caesarean mothers in experimental group and control group. The result shows that there was a signi?cant difference in the maternal outcome among post caesarean mothers in the experimental group after performing early ambulation and there was an association between the maternal outcome among post caesarean mothers with selected demographic variables except in case of period of gestation.