antibody response to MMR vaccine in children with prior exposure to one of its components

The antibody response to MMR vaccine in 20 healthy children with evidence of prior exposure to one of its components either by disease or vaccination was studied using the Haemagglutination inhibition test [HAI] and the Enzyme-linked immunosorbent assay [ELISA]. The results demonstrated that prior exposure to any of the components of the vaccine as evidenced by high antibody titer did inhibit the response to the other components

Evaluation of the antibody coated bacteria in localization of urinary tract infection among Egyptian patients

The fluorescent antibody coated bacteria test [ACB] depends upon the phenomena that the immunoglobulins coat the bacteria present only in the urine of patients with renal infection. The technique of detection of this coating by direct immunofluorescence has been utilized for detection of the site of urinary tract infection. Antibody coated bacteria test was positive in 46 out of 50 cases of pyelonephritis [92%] and in only 6 cases out of 50 cases of cystitis [12%]. IgG was detected in 100% of cases followed by IgA [46%] and IgM [38.5%]. The prevalence of IgG and IgA over the IgM in our study in comparison to the presence of high IgG and IgM in the serum supports the claim of local production of these immunoglobulins by the kidney. From this study we can conclude that antibody coated bacteria test is of good use, rapid, non invasive and reliable mean for localizing the site of urinary tract infection

Bacteriological study of oropharyngeal flora before and after tonsillectomy

In this work the effect of removing the chronically infected tonsils on the pharyngeal flora was studied. Also, a comparison of the bacterial flora of the pharynx in tonsillectomized subjects with that in the healthy non-tonsillectomized ones was performed. All bacteriological work was done according to standard techniques. It is clearly evident from the findings of this study that the presence of chronically infected tonsils, swarmed with streptococcus pyogenes can disturb the normal pharyngeal flora. Tonsillectomy had a profound effect in reducing the pathogenic bacterial flora while the non- pathogenic bacterial flora rapidly reconstituted itself after the operation. Tonsillectomy may be an effective mean for the elimination of bacterial pathogens from the pharynx and so controlling recurrent upper respiratory tract infections

Study the oropharyngeal bacterial flora in breast fed and bottle fed newborns and their mothers

The present work was conducted on 50 newborns and their mothers, 25 breast-fed and the other 25 bottle fed. The first group consisted of 15 newborn infants [7 breast fed and 8 bottle-fed] aged less than one week. The second group consisted of 15 newborn infants [8 breast-fed and 7 bottle-fed] aged from one week to 20 days. The third group of 20 newborns [10 breast fed and 10 bottle fed] aged from 20 days to one month. The swabs were cultured and organisms identified according to the standard bacteriological techniques. The oropharyngeal bacterial flora of the breast fed and bottle fed infants and their mothers were studied and compared. The roles played by the mother, hospital and bottle feeding in the transmission of organisms to the infants were assessed. The importance of breast feeding was emphasized

Detection of hepatitis B surface antigen in pregnant women and in their newborn babies

The presence of hepatitis B surface antigen [HBs] in 100 pregnant women and its transfer to their newborn babies were studied. The presence of hepatitis B e antigen in positive HBsAg carrier mothers, anti-HBe and anti-HBc were also studied using ELISA technique. Among the 100 mothers there were 3 HBsAg positive cariers, but none of them HBsAg to their newborn babies at the time of delivery. Two out of 3 positive HBsAg were positive for anti-HBe. The 3 carriers were negative for HBeAg, 1 out of 3 had anti-HBe and 2 were negative for anti-HBc