ABSTRACT
Radiation is widely used in both diagnostic and therapeutic purposes. The present work aimed at the study of the effect of total body dose irradiation of fast neutrons on the liver of albino rats. The hepatocytic changes were studied histologically, histochemiclaly [including succinic dehyrogenase enzyme, acid and alkaline phosphataes] and ultrastructural examination. The study was carried out on 30 albino rats, 10 were used as control. Each of the 20 rats was exposed to fast neutron whole body irradiation as a single dose for 15 hours receiving a total dose of 300 Rem/rat. The histological and ultrastrutural examination showed a picture of degeneration and necrosis of hepatocytes with pyknosis of the nucleus and vacuolation of the cytoplasm. Both nucleus and cytoplasmic organells were involved in this degeneration. Histochemical results were in accordance and supported the histological results. Succinic dehydrogenase and alkaline phosphatase enzymes showed decreased activity while acid phosphatase enzyme showed an increased activity due to cell necrosis and degeneration. Thus whole body irradiation with fast neutrons as a single dose resulted in both nuclear and cytoplasmic degeneration and necrosis of hepatocytes with a decrease in the liver functions which eventually might lead to cellular death. These changes were mainly degenerative and not carcinogenic
Subject(s)
Animals, Laboratory , Liver/cytology , Histocytochemistry , Microscopy, Electron , MicroscopyABSTRACT
A total number of 20 postmortum prostates-over the age of 50 years-were processed and cut as a whole to get six micron thick paraffin sections, stained by haematoxylin and eosin, Masson's trichrome and peroxidase anti-peroxidase for studying the morphological structure and oestrogen receptors. Six cases were diagnosed as BPH, five as well differentiated adenocarcinoma; four as poorly differentiated adenocarcinoma and five cases looked like normal prostate. The oestrogen receptors in each case were studied