Determinaçao das exigências térmicas de Stomoxys calcitrans (L.) (Diptera, Muscidae), em condiçöes de laboratório / Determination of thermal requirements of Stomoxys calcitrans (L.) (Diptera, Muscidae), under laboratory conditions

Determination of thermal requirements of Stomoxys calcitrans (L.) (Diptera, Muscidae), under laboratory conditions - The biology immature stage of Stomoxys calcitrans (L.) was studied in the laboratory under four constant temperature. The study was carried out in biological incubators at 20, 25, 30 and 35-C; 65 ñ 10% relative humidity and 14 hours of photophase. The most favorable temperature for developing eggs, larval and pupal was 25-C, while 35-C proved to be harmful for a normal developing of S. calcitrans in larval stage. The incubation periods for egg were 69.90, 42.58, 26.10, 21.78 hours and 2.91, 1.77, 1.08, 0.90 days at 20, 25, 30, 35-C, respectively . The larval stage was 18.40, 11.63, 8.55 days and, the pupal stage, 8.60, 4.54, 3.60 days at 20, 25, 30-C, respectively. Threshold temperatures for males were a little higher than for females, however, this difference was lesser than 1-C. On the other hand, the quantity of energy (GD) for developing females was a little higher than for males. No difference was observed between the two methods used for calculating the above mentioned biological parameters of S. calcitrans

Acao antimicrobiana local da associacao anfotericina B e cloridrato de tetraciclina em pacientes portadoras de leucorreia de diferentes etiologias. / Local antimicrobial action of the association of amphotericin B and tetracyclin in patients with leukorrhea of different etiologies

O objetivo do presente estudo e avaliar a eficacia da associacao de cloridrato de tetraciclina 100 mg e anfotericina B 50 mg na forma de creme vaginal em pacientes portadoras de corrimento vaginal. Neste sentido selecionamos no ambulatorio de ginecologia do Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo (Servico do Prof. Carlos Alberto Salvatore), 40 pacientes, cujas idades variaram de 18 a 56 anos (media etaria 31 anos) com corrimento de aspecto inespecifico; 28 destas apresentaram dispareunia; 23 prurido; 22 ardor e disuria.A estas pacientes foi administrada esta associacao sob a forma de creme vaginal, um aplicador por dia, durante sete dias. Nas pacientes que permaneciam com qualquer sintoma, apos este intervalo, o tratamento era prolongado por mais sete dias. Antes do inicio do tratamento e sete dias apos o termino, foram realizados os seguintes exames: exame a fresco com hidroxido de potassio a 10% coloracao de Gram, Giemsa e Papanicolaou para detectar-se flora Gram-negativa, Candida, Trichomonas, Mycoplasma, Chlamydia, Neisseria e Hemophilus