ABSTRACT
Objectives: In this study our main aim is to evaluate the sociodemographic characteristics of non-palpable testes in childrenundergo laparoscopy surgery in Bangladesh.Methods: This cross-sectional observational study was donein the department Pediatric Surgery, Dhaka Shisu (Children)Hospital (DSH), Dhaka, from March 2010 to September 2011.The study included 30 children with nonpalpable testis eitherunilateral or bilateral.Result: During the study, 37% patients belong to 2-5-year agegroup. Followed by 26% in 5-10 years age group, 20% 1-2years age group. (50%) cases, diagnosed by laparoscopy asvas and vessel entered into deep ring underwent plannedinguinal exploration. 40% belong to full term whereas 60%belong to pre-term.Conclusion: From our study we can conclude that,Laparoscopic orchiopexy were done by either single stage ortwo staged Fowler - Stephens method, needs to be evaluatedon large group of patients with longer follow- up.
ABSTRACT
Aims: The tea industry is of great economic importance worldwide, owing to its possession of both food and medicinal values. Bangladesh is among the world-renowned tea exporting countries. However, the inadequate biochemical data for most cultivated Bangladeshi tea genotypes hinders its competitiveness on the world market. This is as a result of previous research mainly revolving around conventional breeding, mutagenesis, and polyploidization. This research aims to characterise the 12 Bangladeshi tea genotypes according to their biochemical content. Such information is inevitable in driving the demand and preference of these tea products on the world market. Study Design: The study was designed based on relevant research articles and standard laboratory procedures. Place and Duration of Study: This research was conducted at the Molecular Biology and Protein Science Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh, from June 2015 to September 2016. Methodology: Reversed-Phase Liquid Chromatography was used to determine the composition of different tea genotypes. A mixture of 999 ml de-ionised water & 1 ml TFA was used as buffer A, and 80% acetonitrile was used as buffer B in RPLC system. Results: We found that all the 12 tea genotypes are rich in Theophylline, Theobromine, Gallic-Acid, and Caffeine content, but with varying quantities. Conclusion: These results indicate that some of these tea genotypes can be used to produce the decaffeinated tea, a newly introduced tea product on the market that is on high demand. To ascertain the diversity of chemical composition among the various tea genotypes, biochemical characterisation of other Bangladeshi tea genotypes should be performed. Such data will enhance the market value and demand for Bangladeshi tea on the world market.
ABSTRACT
The clustered regularly interspaced short palindromic repeats (CRISPR) system was recently identified as a bacterial defense mechanism against phages and plasmids. The CRISPR system is composed of DNA arrays containing short sequences identical to those present in phages and plasmids. These short DNAs are transcribed and processed by CRISPR associated proteins that also guide other CRISPR proteins to target the invading DNA. Only a few of the CRISPR components have been characterized to date, and their mechanism of action is still largely unknown. Phage defense mechanisms probably have co-evolved against the CRISPR system, but none has yet been found. We propose to identify phage genes that counteract the CRISPR system.
ABSTRACT
The snails Lymnaea (Radix) luteola exhibited marked variations in growth, longevity, and attaining sexual maturity at different temperatures and diets. At 10§C, irrespective of foods, pH and salinity of water, the snails had minimum life span, maximum death rate and lowest growth rate. At 15§C, the growth rate was comparatively higher and the snails survived for a few more days. But at these temperatures they failed to attain sexual maturity. Snails exposed to pH5 and 9 at 20§, 25§, 30§, 35§C and room temperatures (19.6§-29.6§C): to 0.5, 1.5 and 2.5 NaCl per thousand at 20§ and 35 §C; to 2.5 NaCl per thousand at 25§C and room temperatures failed to attain sexual maturity. The snails exposed to pH 7 and different salinity grades at 20§, 25§, 30§, 35§C and room temperatures became sexually mature between 25-93 days depending upon the type of foods used in the culture.