ABSTRACT
Brucellosis affects human populations in many developing countries including the Middle East where it is still endemic. The clinical diagnosis of human brucellosis is difficult so, physicians depend upon laboratory confirmation. Peripheral blood specimens were taken from 40 patients having compatible signs and symptoms that were clinically diagnosed to have brucellosis. They were 23 males and 17 females, and their ages range between 21and 74. They were tested by Rose Bengal test [RBT], Standard tube agglutination test [STA] and nested PCR using four primers to amplify 677bp fragments. 23 patients [57.5%] were positive by [RBT]. 25 patients [62.5%] were positive by [STA]. 28 patients [70%] were positive by nested PCR assay. our results show that the PCR assay is more sensitive than conventional serological methods and this coupled with its speed and reduction in risk to laboratory workers. So, PCR technique is a very useful tool for the diagnosis and follow-up of human brucellosis