ABSTRACT
To determine the levels and ratios of serum LH, FSH and Testosterone, among men having history of infertility, A non-interventional descriptive study was carried out in the Department of Public Health Laboratories Division, NIH, Islamabad from January 2004 to December 2005. Two hundred fifty infertile men, classified as azoospermic [50], oligozoospermic [75], asthenozoospermic [50] and normozoospermic [75] were studied for serum LH, FSH and Testosterone, in addition to 50 proven fathers. The respective FSH [mlU/ml] LH [mlU/ml] and Testosterone [nmol/L] levels for the groups were 22.924.15, 13.852.33 and 11.860.70 [Azoospermia], 16.823.79, 10.921.22 and 11.88 +/- 1.06 [Oligozoospermia], 3.220.61, 3.921.17 and 16.24 +/- 2.05 [Asthenozoospermia] while in normozoospermic men were 5.530.52, 7.400.60 and 17.29+1.02, The proven fathers group had 7.74 +/- 0.71 mlU/ml LH, 6.75+1.06 mlU/ml FSH and 15.88 +/- 1.15 nmol/ml testosterone, respectively. The LH/FSH and FSH/LH ratios were 0.77 +/- 0.08 and 1.84 +/- 0.22 [Azoospermia], 1.78 +/- 0.41 and 1.47 +/- 0.18 [Oligozoospermia], 1.51 +/- 0.46 and 1.28 +/- 0.28 [Asthenozoospermia] while in normozoospermic and proven fathers were 1.55 +/- 0.16 and 0.85 +/- 0.10,1.67 +/- 0.22 and 0.94 +/- 0.12. Similarly, the LH/T and T/LH ratios were 1.17 +/- 0.28 and 0.86 +/- 2.70 [Azoospermia], 0.92 +/- 0.28 and 1.08 +/- 0.17 [Oligozoospermia], 0.30 +/- 0.10 and 4.14 +/- 10.4 [Asthenozoospermia] and in normozoospermic were 0.42 +/- 0.08 and 2.34 +/- 0.48.The ratios observed for T/FSH and FSH/T were 0.52 +/- 0.17 and 1.93 +/- 5.95 [Azoospermia], 0.71 +/- 0.28 and 1.42 +/- 3.57 [Oligozoospermia], 5.04 +/- 3.38 and 0.20 +/- 0.30 [Asthenozoospermia] and in normozoospermic men were 2.82 +/- 0.90 and 0.35 +/- 1.12, while in proven fathers were 4.09 +/- 0.57and 1.04 +/- 0.53 respectively. Altered/disturbed concentrations of gonadotropins and androgenic hormones and their ratios cause infertility, since all these hormones act synergistically