ABSTRACT
Diagnosis and quantification of Echinococcus granulosus in-fection in man and animal hosts are centralized to feasible con-trol this study included 93 serum sample, 25 sure positive hydatid cases confirmed surgically, 7 suspected cases diagnosed by indirect haemagglutination IHA and 41 cases other parasitic infection [15 S. mansoni, 8 fasciola, 7 Ascaris, 5 H. nana and 6 Ancylostoma] diagnosed by microscopic examination and were negative by ELISA and/or IHA for anti-hydatid antibody. Twen-ty negative serum samples served as healthy controls. Six types of hydatid fluid antigens [crude, host-free and Con-A purified] of human and camel origin were subjected to electrophoretic separ-ation [SDS-PAGE] and immunoblotting [EITB]. The anti-hydat-id IgG was detected in sera of the different groups for evalua-tion of sensitivity, specificity and diagnostic efficacy each type of antigens. Detection of circulating hydatid antigen [CAg] was performed using anti rabbit hyperimmune sera raised again-st Con-A purified either human or camel hydatid antigen. SDS-PGE revealed several bands ranging from 55-185- kDa with 10kD band shared by all antigens. The specific bands revealed by EITB for Con-A purified camel and human antigens were at 80, 110, 55, 110 kDa respectively. ELISA highest sensitivity [96.9%] was by using host-free Con-A purified glycoprotein fraction of human hydatied antigen. Highest specificity [98.4%] was reco-rded upon use of either Con-A purified camel or human antigen with 94.5% and 97.7 and diagnostic efficacy respectively. Detection of circulating antigen by polyclonal antibodies against Con-A purified human hydatid antigen revealed 91.8%specificity.
Subject(s)
Humans , Male , Female , Humans/surgery , Microscopy , Glycoproteins/blood , Antigens , Antibodies , Enzyme-Linked Immunosorbent Assay , Electrophoresis, Polyacrylamide Gel , Sensitivity and SpecificityABSTRACT
Indirect immunofluorescent technique was utilized to determine the location of butanol extracted microsomal antigen of adult S. Mansoni. Bright fluorescence was located deep in the gut of both adult S. mansoni and S. hematobium treated with specific IgM monoclonal antibody. No fluorescence was observed in any part of schistosomulae. Thus, this butanol extracted antigen may be considered stage and genus specific. The large surface area of the gut allows the detection of enough antigen in the circulation of the host. This antigen has the potentiality to be used as a circulating antigen for diagnosis of active schistosoma infection
Subject(s)
Antigens, Helminth/analysis , Fluorescent Antibody Technique, Indirect/methods , Schistosomiasis mansoni/diagnosis , Schistosomiasis/immunology , Antibodies, Monoclonal/blood , Immunoglobulin M/bloodABSTRACT
Human sera were collected from parasitologically proven cases of Schistosomiasis, Echinococcosis, Fascioliasis and Toxoplasmosis. Using Echinococcus IHA reagent these sera showed cross reaction with Fasciola sera [3.4%] and Schistosoma sera [6.25%]. Using Leishmania IHA reagent against the same sera showed cross reaction against Toxoplasma sera only [6.8%]
Subject(s)
Hemagglutination Tests , Serologic Tests/methods , SerologyABSTRACT
Fifty patients suffering from intestinal schistosomiasis were investigated for detection of antibodies by ELISA and IHA techniques using S. mansoni soluble egg antigen. Higher sensitivity [98%] was obtained using ELISA compared to IHA [78%]. Weak correlation was evident between antibody level measured by ELISA and intensity of infection determined by egg count per gram of stools which makes the assay imprecisely used to assess the prognosis of the disease as the latter depends on the severity of infection