ABSTRACT
Much research has shown that Human Papiloma Virus [HPV] plays an important role in cervix cancer and it is the cause of 99% of cervix cancer worldwide. Lots of research has been done to find a proper method for HPV diagnosis and screening in patients with genital warts. This study aimed at comparing PCR method with Pap smear test in HPV screening. Considering the presence of DNA of HPV, 45 vaginal and cervix swap samples of women with genital warts were tested by means of specific PCR and Pap smear from September 2010 to April 2011. Out of 45 vaginal and cervix swap samples of women suffering genital warts, 37 samples [82.2%] are positive. Of 45 Pap smear samples, 13 [29%] are neoplasia and 32 [71%] normal. The difference between the results of PCR and Pap smear is due to low specification and sensitivity of Pap smear. Thus it is recommended using diagnostic PCR method in addition to Pap smear in order to promote the quality of screening in individuals with genital warts
ABSTRACT
Staphylococcus aureus is the main cause of surgical site infection, causing morbidity and mortality in patients undergoing surgery. Despite a lot of research on the best diagnostic method and Methicillin-Resistant Staphylococcus aureus [MRSA] screening in patients undergoing surgery, the most appropriate diagnostic method is still unknown. The question is whether Rapid Molecular [PCR] or the traditional microbial culture is the most suitable method? This study aimed at systematic reviewing of articles to evaluate molecular method in MRSA diagnosis and the necessity of screening patients before and after surgery to prevent infections and its subsequent outcomes e.g. mortality. Many searches in databases including the digital and medical inlm library and sites such as Science, JAMA,BMD, Springer were done since 2007 to August 2010. In total, 118 studies were selected regarding the following keywords; site surgical infection, Staphylococcus aureus, PCR, and culture. Two independent persons who selected the articles evaluated the designs of the studies and extracted the information using blinded method objectively. After a complete study of other articles, 50 articles were also eliminated, and 8 articles were finally entered the study. Data of culture diagnostic methods and PCR and the statistics of infection prevalence in surgical site were analyzed by Cumulative Pooled analysis. Eight randomized clinical trials of culture methods and PCR had been studied in MRSA diagnosis and screening in different surgeries. The average duration of study was 11.6 months in all articles. The relative risk of side surgical infection was 7.3% with MRSA in all articles and CI was 95% [0.969, 0.367]. The conformity between culture and PCR was 91%, PCR sensitivity 99.2% and PCR specificity 82.2%. The rates of MRSA infection before [0.65%] and after [0.35%] surgery were significantly different with screening. When screening was applied, the rate of infection with 0.95% CI decreased to 28.9% - 31%. Findings confirm the necessity of screening before surgery in order to determine the antibiotic prophylaxis preceding the surgery in those who carry MRSA. Thus, considering the specificity and sensitivity of PCR to microbial culture, molecular method is rapid and effective in diagnosis and screening of the patients who undergo surgery