effect of short term endurance training on the expression of hepatic abcal and reverse cholesterol transport in male wistar rats

The Reverse Cholesterol Transport [RCT] process consists of removing excess cholesterol and phospholipids from peripheral cells to liver to exert as bile. This process consists of removing excess cholesterol by ABCA1 transporter to the Apolipoprotein A-l to form PreBeta HDL, which is then converted to spherical HDL by the action of LCAT enzyme. Previously we have shown that six weeks of endurance training has positive effects on expression of ABCA1. In the present study, we investigate the effect of short term endurance training on ABCA1 expression and other factors in RCT such as Apolipoprotein A-l, Prebeta HDL, LCAT activity and HDL-C concentration. Twenty-eight male wistar rats were subjected to endurance training treadmill running for 3 weeks, 5 days a week, 90 min with 26 m/min during each training session. Expression of hepatic ABCA1 was clearly evident following the 3 weeks of endurance training. The concentration of Apo A-l did not change because of endurance training but the results showed significant increases in PreBeta HDL [P=0/01], LCAT activity [P=0/04] and HDL-C concentration [P=0/004]. The results of this study clearly show that 3 weeks of endurance training with moderate intensity can improve the RCT process and has a positive effect in prevention of arteriosclerosis

effect of treadmill running on plasma and muscle agouti-related protein [AGRP] concentration in male rats

Appetite regulation is one of the most important issues in exercise physiology. AGRP is one of the most important neuropeptide in appetite regulation and energy homeostasis. The aim of this study was to investigate the effect of treadmill running on plasma and muscle [Soleus] concentration of AGRP in male Wistar rats. Forty rats were randomly assigned into two groups. The training group was given exercise on a motor-driven treadmill at 28 m/min [equal to 75% vo2max] for 60 min/day, 5 days/week for 10 weeks. After finishing the exercise protocol, each group was divided into 2 subgroups, the fasting and the fed [n=10] groups. Each subgroup was anesthetized and sacrificed after an overnight fast and the other, after 3 hours of food deprivation. The results showed muscle and plasma AGRP were significantly [P < 0.001] higher in the trained rats in comparison to the control rats. Also there was a significant and positive correlation between Soleus AGRP and plasma AGRP. It can be speculated that negative energy balance as well as local hyperphagia in muscle, induced by exercise produces satiety, signals the hypothalamus, which therefore increases release of AGRP facilitating energy recovery. This mechanism may be involved in glycogen supercompensation as well