ABSTRACT
The aim of thos survey is establishment of an animal model through which a rapid distortion of the hair cell of cochlea administering amikacin and furosemide and using distortion product otoacoustic emission [DPOAE] the functioning of rat's ear would be assessed. Forty-eight male Sprague dawley rats [with average of weight 200-250g, 12 weeks post natal] were randomly divided into six equal groups. Except the control group the rest received 0.5mg/g, 0.75mg/g, 1mg/g, 1.25mg/g, and 1.5mg/g, of subcutaneous amikacin respectively. 30 minutes later every rat received 0.1mg/g of furosemide intrapritoneally. DPOAE of rats was measured prior to these injections and 72 hours later. Then tissue section of the rat's cochlea was prepared.Data were analyzed by means of SPSS [version 17] and ANOVA test and paired sample T test at the significant level of p= 0.05. All the cases had a significant decrease in their DPOAE with the frequencies 2KHz-8KHz [p=0.05].The most change in DPOAE was in rats which had received 1mg/g - 1.5mg/g amikacin. Histological studies approved distortion of hair cell even within the apical turn. To establish a deafness model due to losing hair cells, it is recommended to use a combination of 1mg/g amikacin and 0.1mg/g furosemide. Besides, DPOAE can be used to approve deafness
ABSTRACT
To determine the effects of Ramadan fasting on the secretion of sex hormones in single healthy males, we measured blood hormone levels of testosterone, luteinizing hormone [LH] and follicle-stimulating hormone [FSH] in 52 single male students aged 18-24 years who were fasting for >/= 12 hours during Ramadan. Four blood samples were taken from each, one 2 days before and the others on 10, 20 and 28 Ramadan. Testosterone level was lower, significantly so for the 20th and 28th of the month [P < 0.05]; FSH was increased, significantly for the 20th [P < 0.05]; LH did not change significantly