ABSTRACT
Survey on effects of serotonin agonist on the plasma concentrations of growth hormone [GH] and thyroid hormones [T[3] and T[4]; TH]. Repeated measures [GLM] were used. Twenty Kurdish lambs 3-4 months old. Animals were randomly divided into four groups. The first group [control] received 2 ml normal saline. The second, third and fourth groups received 120, 240 and 480 mg/kg body weight L-tryptophan through jugular vein, respectively. blood samples were collected in three periods including before [days 1, 4, 5 and 6], during [days 7, 8, 9, 10, 11, 12, 13, 14, 15, 18, 22, 26, 30 and 34] and after [days 36, 37 and 38] injections. Plasma concentrations of these hormones in unextracted samples were analyzed by radioimmunoassay kit [Tabeshyarnoor kits company, Tehran, Iran]. Injection of serotonin agonist increased mean plasma concentration [MPC] of GH [P<0.01]. There are no significant differences in MPC of GH within the treated groups. Effects of blood sampling times, period [before, during, after injection] and their interaction with treatment on MPC of GH were significant[ P<0.01]. MPC of TH [T[3] and T[4]] in all of the treated groups was significantly increased compare with control one [P<0.01]. There are no significant differences in MPC of TH within the treated groups. Effects of blood sampling times and period [before, during and after injection] and also their interactive effects with treatment were significant. According to statistical analysis of data we can suggest that serotonin agonist stimulates GH and TH secretion simultaneously
ABSTRACT
Objective: Determination of the Naloxone opioid antagonist effect on the LH and FSH secretion in ewe in non-breeding season
Design: Repeted measures
Animals: Six zandi breed ewes with average [ +/- SE] of weight and age, 42.4 ±4.2 and 760±8 respectively and all of ewes had one birth
Procedure: Ewes were recieved five injections of 0.5 or 0.2 mg naloxone/kg BW and followed by injection of 1 1 g gonadorelin /kg BW. Blood samples were collected every 15 minutes for 4 hours before injections until 4 hours after injections of naloxone and every 15 minutes for 1 hours after injection of gonadroline
Statistical analysis: Analysis of variation
Results: Mean plasma LH concentrations had been significantly increased in both groups after naloxone injections. LH pulses frequencies in both groups significantly increased after naloxone injections. Naloxone failed to alter pulsatile LH amplitudes in both groups. LH base concentrations increased in both groups after injections of naloxone but it was not significant. Also mean plasma LH concentrations had been significantly increased after gonadorelin injection. Mean plasma FSH concentrations, pulsatile frequencies, amplitudes and base concentrations of FSH did not change significantly
Clinical Implications: The results of this study indicate that the opioids may affect on LH pattern secretion in the ewes in nonbreeding season, but the effect of opioid on FSH secretion is not observed