1.
Arch. med. res
;
28(1): 5-9, mar. 1997. ilus
Article
in English
| LILACS
| ID: lil-225190
ABSTRACT
We report a study on the DNA organization in Entamoeba histolytica using a ribosomal DNA probe. The rDNA genes were found forming mers which were separated in a typical ladder pattern by pulse field electrophoresis. DNA rosette structures were visualized through electron microscopy in DNA eluted from bands recognized by the ribosomal probe. The in situ hybridization experiments using a DNA probe suggested that the rDNA genes are portioned between the nucleus and a cytoplasmic structure. These findings provide new data on DNA organization in E. histolytica and open the question concerning the presence of a novel organelle in this eukaryotic parasite
Subject(s)
Animals , DNA, Ribosomal/genetics , DNA, Ribosomal/ultrastructure , Cytoplasm/chemistry , DNA, Protozoan/genetics , DNA, Protozoan/ultrastructure , Entamoeba histolytica/genetics , Entamoeba histolytica/ultrastructure , In Situ Hybridization
2.
Arch. med. res
;
28(supl.1): S1-S329, 1997. tab, ilus
Article
in English
| LILACS
| ID: lil-227426