ABSTRACT
BACKGROUND: Kidney ischemia-reperfusion injury is a common pathophysiological phenomenon in the clinic. A large number of studies have found that the tyrosine protein kinase/signal transducer and activator of transcription (JAK/STAT) pathway is involved in the development of a variety of kidney diseases and renal protection associated with multiple drugs. Edaravone (EDA) is an effective free radical scavenger that has been used clinically for the treatment of postischemic neuronal injury. This study aimed to identify whether EDA improved kidney function in rats with ischemia-reperfusion injury by regulating the JAK/STAT pathway and clarify the underlying mechanism. METHODS: Histomorphological analysis was used to assess pathological kidney injury, and mitochondrial damage was observed by transmission electron microscopy. Terminal deoxynucleotidyl transferase-mediated dUTP nick endlabeling (TUNEL) staining was performed to detect tubular epithelial cell apoptosis. The expression of JAK2, P-JAK2, STAT3, P-STAT3, STAT1, P-STAT1, BAX and Bcl-2 was assessed by western blotting. Mitochondrial function in the kidney was assessed by mitochondrial membrane potential (ΔψM) measurement. RESULTS: The results showed that EDA inhibited the expression of p-JAK2, p-STAT3 and p-STAT1, accompanied by downregulation of the expression of Bax and caspase-3, and significantly ameliorated kidney damage caused by ischemia-reperfusion injury (IRI). Furthermore, the JC-1 dye assay showed that edaravone attenuated ischemia-reperfusion-induced loss of kidney (ΔψM). CONCLUSION: Our findings indicate that EDA protects against kidney damage caused by ischemia-reperfusion through JAK/STAT signaling, inhibiting apoptosis and improving mitochondrial injury.
Subject(s)
Animals , Male , Rats , Reperfusion Injury/drug therapy , Free Radical Scavengers/pharmacology , Edaravone/pharmacology , Signal Transduction/drug effects , Rats, Sprague-Dawley , Apoptosis , STAT Transcription Factors/drug effects , Janus Kinases/drug effects , MitochondriaABSTRACT
Objective@#To explore the effect of mental health education on the problematic social media use among college students, and to provide the basis for college mental health education in colleges and universities.@*Methods@#Problematic Social Media Use was used to select 44 college students who were randomly assigned into experimental and control group. The experimental group received psychological intervention once a week for eight weeks, and the control group did not receive any intervention. Loneliness Scale,Univesity of California of Los Angels(UCLA), Multidimensional State Boredom Scale(MSBS), Interpersonal Anxiety Scale(IAS) were administered before and after the experiment in both of the groups.@*Results@#There was no significant differences between the experimental and the control group in total score of UCLA, MSBS, IAS and AQPMS. After intervention, prevalence of problematic mobile social media use in experimental group decreased from 100.0% to 45.8%. After intervention, significant differences in total score of UCLA, MSBS, IAS and AQPMS were observed in experimental group(P<0.05), as well as between the experimental group and the control group(P<0.05).@*Conclusion@#Mental health education can effectively reduce problematic mobile social media use, as well as negative emotions of college students.