ABSTRACT
The aim of this study was to determine the effect of 9 weeks aerobic exercise and multivitamin supplement on plasma level of HCY, CRP and TNF-alpha in non-athletic obese women. Total 30 sedentary and healthy obese women aged 25-50 year and BMI >/=30 Kg/m2 volunteered for this study. The subjects were randomly categorized into three experimental groups, aerobic group, aerobic-supplement group, and a nonintervention control group. The experimental groups went through a 9-week [3 days per week] aerobic exercise program. Blood samples were taken of all subjects before and after the aerobic exercise program at overnight fast. The data were analyzed through paired t test, ANOVA, and Dant. The significance level was p<0.05. Aerobic and aerobic-supplement exercise decreased the rate of HCY [p=0.013], CRP [p=0.001] and TNF-alpha [p=0.006] in obese women significantly, also the reduction of basic rates of CRP and TNF-alpha in aerobic group [p=0.001] and aerobic-supplement group [p=0.001] were significant compared to control group. But reduction of basic HCY levels was not significant, though. This reduction was significant in aerobic-supplement group [p=0.036]. This prospective data indicate that aerobic exercise with definitive intensity and multivitamin supplement decreased cardiovascular risk factors in obese women. Therefore as a modifiable lifestyle factor should be encouraged in obese adults for prevention of cardiovascular events
ABSTRACT
The aim of this research is investigation of Cu/Zn Superoxide Dismutase enzyme of lymphocytic cell gene expression, total antioxidant status and oxidative stress changes following intensive exercise in young men athletes. This study was a semi-experimental research with a repeated measures design. 20 young men athletes [age range of 21-23 years] participated in this study after signing an informed consent form. Blood sample were collected in pre intensive exercise [grade: 5%, speed: 7/5 mile/h, time: 20 minutes] immediately and recovery [3 hours after exercise test], and Real time-polymerase chain reaction was used for evaluation of Cu/Zn SOD gene expression and autoanalyzer for other markers. H[2]O[2] level and mRNA of Cu/Zn SOD were both increased ,immediately and 3 hours after of exercise [p=0/012, p=0/014], [2.95 +/- 0.84 and 3.37 +/- 0.99] but this changes were not reported significant, but TAS levels are effectively ,raised only in recovery state [p=0/009] [0.86 +/- 0.16]. Intensive exercise increases oxidative stress markers and can weakens the immune system of men athletes, but they raise the activity of antioxidant enzymes in response to threat of free radicals, so Cu/Zn SOD gene expression does not significantly increased
ABSTRACT
The research show that the relationship between antioxidant markers with inflammatory and oxidative stress markers levels changes was not clear, so the purpose of this study is investigation of relationship between total antioxidant status with creatine phosphokinase and hydrogen peroxide in the athlete girls influenced by acute exercise training This study was a semi-experimental research with a repeated measures design and 25 athlete girls within the age range of 21-24 years old volunteered to participate in the research after having expressed their consent through a consent form. Blood sample were collected in the three stages; pre of GXT [Graded exercise test] exercise test [grade: 5%, speed: 12 km/h, time: 20 minutes], immediately and 3 h after exercise test [recovery phase]. Auto analyzer device was used for measurement of total antioxidant status, H[2]O[2] and CPK level, also mixed model methods used for statically analysis. Total antioxidant status [TAS] concentration was faced with a significance increase after of exercise training [p /= 0/065] [0.88 +/- 0.15]. Plasma levels of hydrogen peroxide has not significantly changed after exercise [p >/= 0/255] [2.84 +/- 1.38], and this increase was significant only in the recovery phase [p
ABSTRACT
Inflammatory enzymes and free radicals are important factors affecting the immune system. However, there seems to be no detailed information about the extent to which these factors can affect superoxide dismutase 1 gene expression in female athletes, especially in incremental exercises. Therefore, the aim of the present study was to investigate the correlation between superoxide dismutase 1 gene expression with lactate dehydrogenase [LDH] and free radicals in female athletes after an incremental intensity exercise. Fifteen 22-24 year old female athletes from Urmia, Iran voluntarily participated in the study after completing an informed consent form in 2010. Venous blood samples were collected in three stages: prior to, immediately and 3 h after an incremental exercise [12 km/h at a 5% gradient for 20 min]. Real-time PCR was used to assess superoxide dismutase1 [SOD-1] gene expression as was an autoanalyzer for hydrogen peroxide [H2O2] and LDH concentrations. LDH concentration significantly increased in both stages of the exercise [immediately and 3 h after the exercise], [respectively, P=0.009 and P=0.026], but H2O2 concentration significantly increased only in the recovery phase [P=0.002]. SOD-1 mRNA did not significantly increase in any stage of the exercise [P=0.05]. Moreover, there was only a significant correlation between SOD-1 mRNA and H2O2 increase [P=0.014]. Incremental exercise increased H2O2 and LDH levels in female athletes but only free radicals had a significant effect on SOD-1 gene expression