ABSTRACT
Eighteen strains of Salmonella group E from stool samples were confirmed as Salmonella new serovar. 3, 10 : Z35 : 1, 6 by Centre International des Salmonella, Institut Pasteur, Paris, WHO Collaborating Center for Salmonella, Atlanta, USA and Salmonella-Zentrale Hygienischen Institut, Hamburg, Germany. The name of this new serovar was proposed as S. ratchaburi according to the place of its first isolation in Ratchaburi province. The new serovar of Salmonella was sensitive to many antimicrobial agents except streptomycin and erythromycin.
Subject(s)
Drug Resistance, Microbial , Humans , Salmonella Infections/epidemiology , Salmonella enterica/chemistry , Serotyping , Thailand/epidemiologyABSTRACT
The data for the comprehensive analysis of Salmonella enteritidis infection was based on the information recorded in the request form submitted for Salmonella typing and the results of serotyping at the WHO National Salmonella and Shigella Center, Department of Medical Sciences, Ministry of Public Health, Thailand during 1990-1995. Fifty-one cases of S. enteritidis (SE) infection were confirmed in 1990. The morbidity being markedly increased each year from 1991 to 1995, with 105, 307, 471, 659 and 877 cases being identified, respectively. Among the isolates of Salmonella spp, SE was the most common serotype during 1993-1995. The cases were mostly predominant in Bangkok and then in southern, northeastern, central and northern regions, respectively. The morbidity rates per 100,000 population per year of SE infection in 1990-1995 were 0.09, 0.18, 0.53, 0.8, 1.11 and 1.47, respectively. Analysis of the infection rate by sex and age demonstrated that the incidence in males was higher than in females. Adult patients were more frequently found than adolescent patients. The organism was found in feces, blood, pus, urine, cerebrospinal fluid and sputum, accounting for 41.7, 35.8, 5.5, 2.5, 1.7 and 0.4%, respectively. The average extraintestinal isolation index was 0.52.
Subject(s)
Adolescent , Adult , Age Distribution , Aged , Child , Child, Preschool , Female , Humans , Incidence , Male , Middle Aged , Salmonella Infections/epidemiology , Salmonella enteritidis , Sex Distribution , Thailand/epidemiologyABSTRACT
The Salmonella specific DNA fragment from genomic DNA of S. typhimurium ATCC 23566 was cloned in E. coli and successfully used as a digoxigenin labeled probe for detecting the presence of Salmonella serotypes in both artificially contaminated food and natural contaminated food samples.
Subject(s)
Cloning, Molecular , Colony Count, Microbial , DNA Probes/genetics , DNA, Bacterial/genetics , Digoxigenin , Escherichia coli/genetics , Food Microbiology , Humans , Immunoblotting , Salmonella Food Poisoning/microbiology , Salmonella typhimurium/geneticsABSTRACT
The first isolation of Salmonella blockley in Thailand was found in 2 strains of animal feed samples and 3 strains of chicken feather samples from a private poultry company in 1989. From 1987 to 1992, the number of S. blockley isolates increased and found in various sources. The major sources were the stools of diarrheal patients, mainly children. Another source of S. blockley was frozen chicken meat which increased every year studied. S. blockley isolated from human and other sources showed a high percentage resistance to streptomycin, tetracycline, kanamycin and chloramphenicol and a low percentage resistance to ampicillin and cotrimoxazole. Thus, S. blockley must now be listed as a possible cause of Salmonella food poisoning in Thailand.