Potent Anti⁃bacteria Activity of Tamsulosin Hydrochloride and Ningmitai Capsule in Combination by Regulating the Gene Expression Related to β⁃Lactamase, Hemolysis, and Virulence / 中国生物化学与分子生物学报

Tamsulosin hydrochloride (TSH) is a selective α

Sodium Arsenite Inhibits Proliferation and Induces Apoptosis of Human Hepatic Cells / 中国生物化学与分子生物学报

Arsenic is a potent toxic heavy metal found in the environment that can causes health problems, including liver disease in humans and animals. Chronic exposure to arsenic remains an environmental health problem worldwide, affecting hundreds of millions of people. Although the oxidative stress and apoptosis induced by arsenic have been confirmed, the underlying mechanism of apoptosis has not been fully elucidated. The purpose of this study is to investigate whether sodium arsenite (SA)induced liver toxicity is related to the regulation of DNA replication and repair pathways. The results of MTT and microscopy showed that SA has an inhibitory effect on the proliferation of human hepatocytes (L02), and this effect is time and concentration dependent. Flow cytometry detected the effects of different concentrations of SA on L02 cells. Compared with the control group, high concentrations of SA significantly affected the L02 cell cycle. In addition, RNA sequencing results showed that the differentially expressed genes in cells after SA treatment were concentrated in the DNA replication process and repair pathways. The effect of SA treatment on the expression of human RECQ DNA helicase and repair genes was further confirmed by Western blot and real-time quantitative PCR. In vitro study showed that SA treatment inhibited cell proliferation, and induced apoptosis as well as DNA damage and cell cycle arrest of human liver cell L02. Collectively, these results indicate that arsenic poisoning is related to the regulation of DNA replication and repair pathways, which provides insight for understanding the molecular mechanism of arsenic poisoning.

Correlation of monitoring the renal pelvic pressure in RIRS and the morbidity of postoperative fever / 局解手术学杂志

Objective To investigate the clinical significance of monitoring the renal pelvic pressure( RPP) and regulating the manual perfusion pressure in flexible ureteroscope holmium laser lithotripsy. Methods A total of 189 patients with upper urinary tract calculi treated by RIRS in our hospital were retrospectively analyzed from August 2014 to August 2017. The renal pelvic pressure was monitored during RIRS in 136 cases( monitored group) whereas no monitoring occurred in the rest 53 cases( unmonitored group) . The monitored group was divided into two sub-groups of 49 cases named high-pressure group(the cumulative time of renal pelvis pressure upon 40 cmH2O≥1 min) and 87 ca-ses named low-pressure group respectively. The morbidity of postoperative fever ( T≥38. 5℃) was evaluated statistically between monitored group and unmonitored group,meanwhile between high-pressure group and low-pressure group. Results Postoperative fever did not correlate to age,sex,involved kidney,and postoperative urinary tract infection. Whether renal pelvic pressure was monitored or not,infection calculi, duration of operation and whether the cumulative time of renal pelvis pressure upon 40cmH2O≥1 min contributed to postoperative fever. The rate of postoperative fever in unmonitored group was higher than monitored group while the same between high-pressure group and low-pres-sure group,with statistically significant difference(P<0. 05). Conclusion Monitoring the intraoperative RRP and regulating the manual perfusion pressure during RIRS has positive significance in postoperative recovery and contribute to reducing postoperative fever.

Simultaneous determination of inorganic elements in stems and leaves of Scutellaria baicalensis from different regions by ICP-MS / 中草药

Objective An inductively coupled plasma mass spectrometry (ICP-MS) method was established for the determination of inorganic elements in stems and leaves of Scutellaria baicalensis. The inorganic elements in the extracts were analyzed and evaluated. Methods ICP-MS was applied to determine 23 kinds of inorganic elements in samples digested by microwave in stems and leaves of S. baicalensis from eight regions, and the results were analyzed by principal component analysis (PCA) and correlation analysis. Results There were no differences in the types of inorganic elements in stems and leaves of S. baicalensis from different regions, but the content of them varied greatly. Among them, Fe, Zn, Cu, Mn, Cr, Co, Ni, Sr, B, and Ni were essential elements of the human body. The content of Al and Fe was the highest, and the content of B, Ti, Mn, Sr, and Ba was higher than others. The total content of heavy metals and harmful elements of samples were up to the mustard, except S10. The contents of Cd in S2, S10 and S22-S30 exceeded standards. Thirty-five batches stems and leaves of S. baicalensis were divided into eight groups, which were analyzed by thePCA with the contents of 23 elements as variates. These regions which have different geographies and climates might lead to the differences in the contents of inorganic elements in the samples. Four factors (F1-F4) were selected to make a comprehensive evaluation. The evaluation function was F = 0.444 2 F1 + 0.166 6 F2 + 0.129 1 F3 + 0.056 4 F4. The results showed that the scores of samples from Shanxi and Shaanxi were higher, which indicated that the qualities of above samples were better than those of other samples. Conclusion In this study, an accurate and efficient method for the analysis and determination of inorganic elements in stems and leaves of S. baicalensis from different regions was established, which provided a scientific reference for the quality control, safety evaluation, and comprehensive utilization of S. baicalensis resource.

Optimization of drying process for Scrophulariae Radix by multivariate statistical analysis / 中国中药杂志

To establish the suitable modern drying processing parameters for Scrophulariae Radix (SR). With reference to the traditional drying processing method of SR and the characteristics of modern drying equipment, the drying process for SR was simulated as the following three stages: temperature-controlled drying-tempering-temperature-controlled drying. Eighteen batches of SR samples were obtained by the drying methods after the orthogonal design experiment with seven factors namely temperature, wind speed, and target moisture for the first stage, tempering time and temperature, as well as temperature and wind speed for the second stage. UPLC-TQ-MS was applied for determination of nine target compounds including catalpol, harpagide, verbascoside, ferulic acid, angroside-C, aucubin, harpagoside, cinnamic acid and ursolic acid in those dried samples and another 19 batches of SR samples collected from genuine producing area. Principal Component Analysis (PCA) was performed, and total energy consumption was also taken into consideration for analysis and evaluation. Results showed that the optimal drying processing method for SR was as follows: drying temperature of 60 ℃, drying wind speed of 50 Hz, and 50% for target moisture in the first stage; 24 h for tempering time and temperature of 20 ℃ in the second stage; drying temperature of 60 ℃, and drying wind speed of 30 Hz in the third stage. The medicinal materials with optimized modern drying processing method were extremely similar to those collected from genuine producing area in the aspect of both external properties and target compounds, and they were in line with the 2015 version of "Chinese Pharmacopoeia" requirements. In addition, they could help to shorten the drying time and increase the efficiency of primary processing, and thus promote the normalization and standardization of primary drying processing for SR.

T2 mapping of articular cartilage in knee osteoarthritis using a magnetic resonance staging / 中国医学科学院学报

<p><b>OBJECTIVE</b>To evaluate T2 mapping of articular cartilage in knee osteoarthritis (OA).</p><p><b>METHODS</b>Totally 38 healthy subjects (group H) and 53 OA patients received scoring with the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC)and underwent T2 mapping of tibiofemoral articular cartilages. The T2 values in 10 subregions of the cartilages were measured. Patients in the OA group were further divided into OA1 group and OA2 group using the modified Magnetic Resonance Recht Grading System. In OA group, the fat-suppressed three-dimensional fast spoiled gradient echo MRI was performed to obtain the modified Whole-Organ Magnetic Resonance Imaging Score (WORMS). The differences of T2 values among group H, group OA1, and group OA2 were compared. The correlation between T2 value and WORMS/WOMAC scores was analyzed. The intra- and inter-observer reproducibility of measurement was calculated.</p><p><b>RESULTS</b>The T2 values in all the subregions ranged 43.9-53.6ms in group H, 41.1-55.0 ms in group OA1, and 45.6-56.1ms in group OA2. T2 values in group OA2 were significantly higher in central medial femoral subregion, central medial, and lateral tibial subregions compared with group H, also significantly higher in central medial femoral subregion, anterior and central medial tibial subregions compared with group OA1 (P<0.05). T2 values were significantly correlated with WORMS scores (R=0.307-0.811, P<0.01) except in posterior lateral femoral subregion, but not with WOMAC scores. The correlation coefficients for intra- and inter-observer measurement showed good reproducibility (R>0.740, P<0.05) except in anterior lateral tibial subregion for inter-observer of measurement.</p><p><b>CONCLUSION</b>T2 mapping can differentiate the OA severity of knee cartilage using a magnetic resonance staging, and therefore can be a sensitive technique for monitoring the severity of OA.</p>

Clinical study of human cytomegalovirus infection in colony forming unit-megakaryocyte in patients with idiopathic thrombocytopenic purpura / 中华儿科杂志

<p><b>OBJECTIVE</b>Idiopathic thrombocytopenic purpura (ITP) is a hemorrhagic disease in children with blood platelets redundant destruction caused by chaotic immunological mechanism. However, some patients with ITP with negative platelet-associated antibody and ineffective adrenal cortical hormone therapy probably have special pathogenesis. It is indicated that the human cytomegalovirus (HCMV) can incubate in haemopoietic stem cell/ancestral cell to inhibit its generation and differentiation. Therefore, the study was designed to investigate HCMV-late mRNA expression in megakaryoblast for the purpose of examining the pathogenesis of ITP and to examine the effectiveness of ganciclovir on ITP.</p><p><b>METHODS</b>Colony forming unit-megakaryocyte (CFU-MK) of 46 ITP patients with HCMV infection were incubated. Reverse transcription-polymerase chain reaction (RT-PCR) was subsequently used for HCMV-late mRNA detection. Ganciclovir therapy was given to both positive group and negative group for comparison of therapeutic effectiveness.</p><p><b>RESULTS</b>Nineteen out of 46 CFU-MK culture cell specimens with positive HCMV-DNA by PCR or positive CMV-IgM by enzyme linked immunosorbent assay (ELISA) from serum of peripheral blood showed positive for HCMV-late mRNA. While, the remaining 27 were negative. Sixteen positive responders to ganciclovir therapy were observed amongst those with positive HCMV-DNA. Whereas, only 4 positive responders to ganciclovir therapy were noticed amongst those with negative HCMV-DNA. The curative effectiveness in positive group was significantly higher than that in negative group (P < 0.01).</p><p><b>CONCLUSION</b>HCMV can directly infect CFU-MK, which might be one of the mechanisms responsible for ITP. Ganciclovir is an effective therapy resulting in an increase in thrombocyte in ITP patients whose HCMV-late mRNA was positive in their CFU-MK.</p>

Changes of Plasma Brain Natriuretic Peptide Concentration Induced Therapy by Daunorubicin and Its Clinical Significance in Children with Acute Leukemia / 实用儿科临床杂志

Objective To explore the clinical significance of the brain natriuretic peptide(BNP) in evaluating the cardiotoxicity caused by daunorubicin(DNR) through studying the changes of the plasma BNP levels in children with acute leukemia who accepted the chemotherapy with DNR.Methods Thirty-one children with acute lymphoblastic leukemia(ALL) admitted in the year of 2002-2004 underwent the chemotherapy in DVLP project.The plasma level of BNP was measured by enzyme linked immunosorbent assay(ELISA) and the left ventricular end diastolic diameter(LVEDD) by color Doppler respectively before and after the administration of DNR.Simultaneously,electrocardiography(ECG) and cardiac muscle enzymes(LDH1,CK-MB) were measured as routine.Results The plasma level of BNP increased from(3.97?2.41) ng/L to(18.25?7.63) ng/L(P

MAPK signal pathway plays a role in proliferation of Jurkat cells induced by ouabain / 中国实验血液学杂志

The object was to study the effect of ouabain on Jurkat cells and its possible mechanism. The effect of ouabain of low concentration on Jurkat cells was confirmed by MTT, while c-myc gene transcription was measured by RT-PCR, and the phosphorylation of MAPK (ERK1/2) as well as the expression of c-myc gene was tested by Western blot respectively. The results showed that ouabain at low concentration could induce the proliferation of Jurkat in a time-and dose-dependent manner. At the same time, the phosphorylation of MAPK (ERK1/2) and the expression of c-myc gene was enhanced. In conclusion, ouabain stimulates the intracellular MAPK signal pathway by acting on the Na, K-ATPase, and thus induce the proliferation of Jurkat cells, in which the regulation of c-myc gene expression may be involved.