ABSTRACT
Oncogene StarD4 had the function of promoting proliferation and metastasis of triple-negative breast cancer (TNBC), but its clinical value and molecular mechanism are unknown. This paper found that StarD4 was highly expressed in cancer tissues of TNBC patients, and higher expression level of StarD4 in TNBC patient resulted in poorer prognosis. Based on transcriptomics of MDA-MB-231 cell model, the results of bioinformatics analysis showed that down-regulated expression level of StarD4 led to overall downregulation of cholesterol-relative genes and significant enrichment of cancer mechanism and pathway. Further analysis and investigation verified that StarD4 might cross-promote the protein stability of receptor ITGA5 through the cholesterol pathway to enhance TNBC progression, which provides guidance for clinical application of TNBC diagnosis and treatment.
Subject(s)
Female , Humans , Breast Neoplasms/genetics , Cell Line, Tumor , Cell Proliferation , Lipids , Membrane Transport Proteins , PhosphoproteinsABSTRACT
Objective To investigate the expression of HLA-DR and distribution of dendritic cells(DC) in endometrial carcinoma and to evaluate their influence on clinical staging and prognosis.Methods The 86 cases of patients with endometrial carcinomas were detected by immunohistochemical SP method for HLA-DR and S100 positive dendritic cells,and the predictive value in clinical stage and prognosis were analyzed.Results The positive rate of HLA-DR expression was 27.9%(24/86),which was decreased with the increase of clinical staging(P0.05).There were 46.5%(40/86) of the cases with a dense infiltrate(≥10/HPF) of S100 positive DC.There was significant difference on the density of DC infiltrating among the patients with different clinical stages and prognosis(all P
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Objective To evaluate the diagnostic accuracy of imprint cytology for intracranial tumors during operation. Methods 150 cases of intracranial tumors were diagnosed by imprint cytology. The imprint was prepared by touching the specimens several times with clean slide, and then the slides were fixed and stained with HE. The diagnostic results obtained by imprint cytology were compared with those made by frozen and routine paraffin sections. Results The accurant rates of diagnosing for non-glial tumors, classifying for incracranial tumors and grading for glial tumors were 100%, 91.3% and 88.3%, respectively. In addition, the morphological features of glial tumors, meningioma, pituitary adenoma on imprint cytology were briefly described. Conclusion The accurant rate of imprint cytology is almost equal to that of frozen section, and both the detection methods have a supplementary effect to each other.
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Objective This study was designed to investigate the expression of human telmerase reverse transcriptase (HTERT) in nasopharyngeal carcinoma (NPC) and its clinical significance.Methods Expression of HTERT in NPC and dysplasia of nasopharyngeal epithelium and chronic inflammation of nasopharyngeal epithelium were determined by immunocytochemical staining.The results were analysed,compared with the clinical TNM stage ,lymph node metastasis ,other places metastasis and prognostic survivals of the NPC patients.Results Positive rate of HTERT in NPC was 83 7%(70/84),while positive rate of HTERT in dysplasia of nasopharyngeal epithelium and chronic inflammation of nasopharyngeal epithelium both were 0 0%(0/8 and 0/16) (P0 05).The median survival of HTERT positive group was shorter than the nagative one.Conclusions Expression of HTERT may be a potential marker for clinical diagnosis of NPC and may has significance in predicting clinical TNM stage, metastasis and prognostic survival of the NPC patients.