ABSTRACT
Objective Hes5 is one of the critical effectors of the Notch pathway and its expression Call inhibit neuronal differentiation.Which protein expression is influenced in cell differentiation by HeS5 remains to be determined.Methods Experiments were designed to study changes in protein expression in the subventricular zone(SVZ)of the HesS knockout mice at the postnatal day 8 using the new method of proteomic isobaric tags for relative and absolute quantification(ITRAQ),and confirmed expression of the up-gnlated and down-regulated proteins by Western blotting.Results Nineteen proteins showed differences in expression levels in the SVZ of Hes5 knockout mice.When compared tO the wild type mice,expressions of 11 proteins in the knockout mice were up-regulated,and the differentially expressed proteins showed ratios of knockout/widtype between 1.20 and 1.32.Expressions of 9 proteins were down-regulated,with their ratios were between 0.77 and 0.83.The upregulated proteins have been shown to be involved in neurogenesis,cell adhesion,migration and signal transduction.The downregulated proteins have been mainly shown to be involved in growth inhibition and cytoskeleton.Westem bloning analysis further confirmed the expression of proteins from iTRAQ.Conclusion Our findings suggest that Hes5 may influence cell differentiation by regulating expression of some proteins involved in cell growth and migration,and its cytoskeleton.
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AIM: To study the apoptosis induction of cyclooxygenase - 2 ( COX - 2) inhibitor, celecoxib and adriamycin (ADM) on tumor apoptosis of gastric carcinoma MGC - 803 cells, and to explore their possible molecular mechanism(s) and interactions. METHODS: The number of MGC - 803 cells was observed by MTT assay. Tumor apoptosis was studied by fluorescence microscopy, flow cytometry (FCM), and DNA ladder. RESULTS: MGC -803 cell number was significantly decreased with increasing dose of ADM. Cells were accumulated in G0/G1 phase and the number of cells in S phase was decreased. ADM (5 mg/L) combined with celecoxib (25 μmol/L) markably inhibited the growth of MGC - 803 cells. Significant morphological changes of typical apoptosis were observed after treatment with combined use of celecoxib and ADM. Compared with ADM or celecoxib alone, ADM plus celecoxib obviously enhanced the DNA ladder fragment revealed by agarose gel electrophoresis of DNA. After exposure to combined celecoxib and ADM treatment for 48 h, MGC - 803 cells were accumulated in G0/G1 phase. There was a decrease in the number of cells in S phase as compared to celecoxib or ADM alone. CONCLUSION: Celecoxib and ADM appear to have synergistic effects for the apoptosis induction. This may be an important prospect for applying COX - 2 inhibitors to assist chemical therapy of ADM in clinical use.
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AIM and METHODS: The immunohistochemical method was used to study the on synapsin ex- press in hippocampal formation during spatial learning and memory in rats. RESULTS: (1 ) In control group there are diffuse brown granules without any obvious granular product in hippocampal fornation and in model group there are synapsin products in the hippocampal formation, especially in the subregion CA3, CA4 and the dentate gyrus after water maze flamed for one week. After two weeks water maze thened the hippocampal formation appear the same dis- tribution of the product edes with darker staining. (2) The light dendity of synapsin products between the model and the control groups showed significant differences, that in the model group was highter than that in the control group (P
ABSTRACT
Objective Hes5 is one of the critical effectors of the Notch pathway and its expression can inhibit neuronal differentiation.Which protein expression is influenced in cell differentiation by Hes5 remains to be determined. Methods Experiments were designed to study changes in protein expression in the subventricular zone(SVZ) of the Hes5 knockout mice at the postnatal day 8 using the new method of proteomic isobaric tags for relative and absolute quantification(iTRAQ),and confirmed expression of the up-gulated and down-regulated proteins by Western blotting. Results Nineteen proteins showed differences in expression levels in the SVZ of Hes5 knockout mice.When compared to the wild type mice,expressions of 11 proteins in the knockout mice were up-regulated,and the differentially expressed proteins showed ratios of knockout/widtype between 1.20 and 1.32.Expressions of 9 proteins were down-regulated,with their ratios were between 0.77 and 0.83.The up-regulated proteins have been shown to be involved in neurogenesis,cell adhesion,migration and signal transduction.The down-regulated proteins have been mainly shown to be involved in growth inhibition and cytoskeleton.Western blotting analysis further confirmed the expression of proteins from iTRAQ.Conclusion Our findings suggest that Hes5 may influence cell differentiation by regulating expression of some proteins involved in cell growth and migration,and its cytoskeleton.
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0.05) 1 day after acupuncture.There was a significant difference in the damage neurons of penumbrazone between the acupuncture and control groups(P