ABSTRACT
Objective To adopt Suitability Assessment of Materials for suitability assessment of domestic Kegel exercise online health education written materials. Methods After Searching Kegel exercise online health education written materials in search engines, four health education professionals assessed the suitability of the collecting materials.One professional evaluated all collecting materials,and all materials were divided into three parts. The other three professionals evaluated one part agian independently.Excel and SPSS 19.0 were used to analyze the results. Results 38 Kegel exercise online health education written materials were collected.2(5.3%)of the materials were assessed as"Superior",23 (60.5%) of the materials were "Adequate", and 13 (34.2%) of the materials were evaluated as "Not suitable". Average percentage score was 44.05% (24% ~74% ), and the overall assessment was"Adequate". Conclusion Most domestic Kegel exercise online health education written materials′quality should be certified and suitability were adequate,which need some improvement.
ABSTRACT
cDNA of human interleukin-8 was expressed in E. coli bacteria, and recombi-nant human interleukin-8 ( rhIL- 8 ) was produced. The latter was used for treatment of rat intestinal ischemia-reperfusion(I-R) injury, and it was found that rhIL-8 significantly improved the hypotensive state of the animals,and it also inhibited the elevation of plasma endothelin level , decreased the intestinal myeloperoxidase activity, and obviously prolonged the survival timeof the animals. Perfusion of isolated rat superior mesenteric artery with rhIL-8 markedly inhibited endothelin synthesis or (and) release induced by anoxia. The above results suggest that rhIL- 8 may be an effective preventive and therapeutic agent for I-R injury through its inhibition on leukocyte adhesion and endothelin synthesis or (and) release.
ABSTRACT
By using genetic engineering techniques, we had successed in the expression of recombinant human interleukin 8 (rhIL-8)in E. col(?) IL-8 cDNA insert into the plasmid pGEM-IL-8 was subcloned into expression vector pMS316, and IL-8 fusion protein was produced in E.coli with high efficiency, The purified recombinant protein exhibited chemotactic activity for mouse neutrophils both in vive and in vitro. It showed that a fragment of the human IL-8 had no influence on the chemotactic activity of IL-8.