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Objective To investigate the curative effect of short segment of unilateral laminectomy decompression of intervertebral bone graft fusion with pedicle screw fixation in treatment of lumbar spine instability.Methods 100 cases with lumbar instability were randomly divided into the study group and control group with 50 cases in each group,the study group was treated with short segment of unilateral laminectomy decompression of intervertebral bone graft fusion with pedicle screw fixation for treatment,while the control group was treated with conventional methods of treatment.The intraoperative,postoperative situation and clinical efficacy of the two groups were observed and compared.Results The operation time,intraoperative bleeding,drainage,hospital stay and cost of hospitalization of the study group were (121.31 ± 13.73) min,(151.24 ± 24.65) mL,(35.34 ± 6.79) mL,(12.03 ± 3.22) d and (22 012.23 ± 3 018.67)yuan respectively,which were significantly lower than those of the control group[(161.32 ± 11.60) min,(201.32 ± 25.46) mL,(102.35 ± 11.87) mL,(19.42 ± 4.31) d and (26 278.65 ± 3 216.53) yuan] (t =9.829,10.829,7.984,10.983,9.593,all P ＜0.05).The excellent and good rate of the study group was 92％,which was significantly higher than 70％ of the control group (x2 =13.927,all P ＜ 0.05).Conclusion Short segment of unilateral laminectomy decompression of intervertebral bone graft fusion has a high curative effect in the treatment of lumbar instability,with short operation time and hospitalization time,less blood loss and postoperative drainage volume,low medical cost,which is a good operation scheme.
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AIM: To investigate the protective effect of crude Paecilomyces tenuipes polysaccharides(cPtPs) and Paecilomyces tenuipes polysaccharides(PtPs) in a rat model of acute hepatic necrosis induced by carbon tetrachloride(CCl4).METHODS: Wistar rats were divided into four groups(control group,CCl4 group,cPtPs+CCl4 group and PtPs +CCl4 group),the four groups were given intragastrically with normal saline,cPtPs and PtPs for 15 d,respectively.In the last two days,these groups except control group were injected peritoneally with CCl4.Serum levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),total bilirubin(TBIL),direct bilirubin(DBIL) and indirect bilirubin(IBIL) were detected by automatic biochemistry analyzer.Pathological changes of hepatic tissue were assessed by hematoxylin-eosine(HE) staining.The levels of superoxide dismutase(SOD) and malondialdehyde(MDA) in liver homogenate were analyzed using xanthinoxidase and thio-barbituric acid,respectively.The concentration of Ca2+ in hepatocyte mitochondria was determined by colorimetric method.The expression of ?-smooth muscle actin(?-SMA) was examined in hepatic tissue by immunohistochemical method.RESULTS: Compared with control group,serum levels of ALT,AST,TBIL,DBIL and IBIL in CCl4 group increased significantly,denaturation and necrosis implicated to the whole hepatic lobules.Compared with CCl4 group,serum levels of ALT,AST,TBIL,DBIL and IBIL in PtPs +CCl4 group decreased significantly,denaturation and necrosis located in the third region of hepatic lobules,the level of SOD increased and MDA decreased(P
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Objective To investigate the physicochemical properties,structural characters and antitumor activity of a polysaccharide PTPS-1 from Paecilomyces tenuipes.Methods PTPS-1 was obtained by DEAE-cellulose and Sephadex G-100 chromatography column.The methods of HPLC,GC,IR,and NMR were used to investigate the molecular weight,monosaccharide composition,and structural characters of PTPS-1.The CCK-8 kit was used to determine the proliferation of PTPS-1 on murine splenocyte and the supernatant of the murine macrophage with PTPS-1-stimulated on Sp2/0 cells.Results PTPS-1,whose molecular weight was 1.84?104 and purity was 98.977%,contained mannose(Man) and galactogen(Gal) as monosaccharide constitute in molar ratio of 1.3∶1.It consisted of a backbone of ?-(1→6),?-(1→2),and ?-(1→3) linkage,whose branches were made by Gal-(1→3) linkage.PTPS-1 significantly stimulated the murine splenocyte proliferation and the supernatant of macrophage inhibited the growth of Sp2/0 cells in vitro.Conclusion PTPS-1 is a polysaccharide from P.tenuipes with immunomodulatory and antitumor activity.