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Objective To explore the feasibility of tracking migration and distribution of SD-rat adipose derived stem cells (ADSCs) labeled with Polyethylene Glycol/Polyehthyleneimine modified superparamagnetic iron oxide (PEG/PEI-SPIO) in rats with chronic cerebral ischemia using MRI.Methods Thirty female SD rats underwent permanent occlusion of bilateral common carotid arteries 6 months were divided into PEG/PEI-SPIO labeled group and unlabeled group (each n =15).Labeled or unlabeled ADSCs suspension was injected into the right ventricle of rats in two groups,respectively.MR scans were performed at the 7th,14th and 21st day after transplantation for each 5 rats.T2 value of T2mapping sequence in hippocampus,cortex and cerebellum were measured.Then the rats were scarified,and the brains were obtained,and Prussia dyeing was performed.Under high magnification,blue dye cells at each time points and brain area were counted.T2 values and blue dye cells were statistically analyzed.Results Class round hypointensity areas were detected in temporol-parietal cortex and hippocampus in both groups on T2WI,T2* WI and SWI.T2 value of the right temporolparietal cortex and hippocampus in the labeled group was shorter than those of the unlabeled group on the 14th day after transplantation (P=0.013,0.045).T2 value of the right temporol-parietal cortex in the labeled group was shorter than that of the unlabeled group on the 21st day after transplantation (P=0.007).The number of blue dye cell of the right temporol-parietal cortex on the 14th and 21th day,hippocampus on the 14th day in the labeled group were more than those of the unlabeled group after transplantation (P=0.029,0.032,0.043).Conclusion ADSCs labeled with PEG/PEI-SPIO transplanted into lateral ventricle of SD rat could migrate to the damaged areas caused by chronic cerebral ischemia.It is possible to use quantitative MRI to track migration and distribution of ADSCs labeled with PEG/PEI-SPIO in rat brain.
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Objective To observe the effect of cigarette smoking on cutaneous wound healing in rats.Methods Healthy adult SD rats,weight 200-250 g,were randomly divided into 4 groups:control group (group C),low tar content group (group L),middle tar content group (group M) and high tar content group (group H),ten rats each group.4 full thickness dermal excision wound models with the diameter of 2.0 cm were established on the back of 40 healthy male or female SD rats.The wound spacing was greater than 2 cm.Group C did not do any intervention treatment,Groups L,M and H rats were put,respectively,into homemade smoke box in 0.1,0.5,and 0.8 mg different-grade tar content of cigarettes and smoked,and the time of wound healing was observed.The wound granulation tissue was taken in 3 and 7 days after surgery.The expression of CD68+ macrophages was detected by immunohistochemical method.The wound healing rate was observed on the days 7 and 16.Results Compared with group C,wound healing time in groups L,M and H was extended (P＜ 0.05).Immunohistochemical results showed that macrophage numbers in groups L,M and H wound were significantly less than that of group C.The wound healing rates 7 days and 16 days after surgery in the three group smoked were significantly lower than that of group C.Conclusions Cigarette smoking leads to wound healing delay,and wound healing rate is reduced at fixed time point,with the decrease in the number of macrophages.
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Objective The study combined multiple local flaps to repair small or medium-sized area of facial skin and soft tissue defects,and to explore the advantages of the local flap collaboration repair.Methods Following the partition repair principle and depending on the wound size and location of these defects,the appropriate local flap was selected to cover the wound.Therefore,the incision was hidden and conformed to the face natural contour or skin wrinkles.Results 77 patients were treated in this group,74 cases got phase] healing and the other 3 got phase Ⅱ healing; all were followed-up for 1 to 8 months.The repair flap and surrounding skin had good similarity in terms of color,texture and contour,the incisions were hidden and the scars were not obvious; no tumor had recurred.The face contour and visual effects were good,and the aesthetic results were satisfactory.Conclusions The combined application of several different local skin flap to repair facial trauma defects,can not only reduce the overall difficulty and risk,reduce surgical complications,but also improve the repair quality,to maximize the recovery of facial aesthetic appearance.
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Objectives To investigate factors affecting neonate birth weight. Methods Random cluster sampling method was adopted to investigate the physical development of 5539 single live newborns in Beijing, Harbin, Changsha, and Guang-zhou. Single factor analysis and logistic regression analysis were used to find the factors influencing neonate birth weight. Re-sults Single factor analysis showed that neonatal sex, gestational age, maternal age, maternal pre-pregnancy BMI, gestational weight gain, mother's education and occupation have effects on neonatal birth weight. Risk factors for macrosomia, including male fetus, maternal age≥25 years before pregnancy, maternal pre-pregnancy BMI≥24 kg/m2, gestational weight gain greater than 12.5 kg, and preterm delivery and maternal pre-pregnant BMI<18.5 kg/m2 are the risk factors of low birth weight. Conclu-sions Premature, excessive weight gain during pregnancy, high or low maternal pre-pregnancy BMI are main factors that caused abnormal body mass in neonates.
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Objective To understand the birth conditions of newborns in four cities of China and its trend. Methods The physical development data of single live newborns in 4 cities of Beijing, Harbin, Changsha and Guangzhou were investi-gated by random cluster sampling method, and was compared with the national neonatal growth standard developed from the investigation in 15 cities from 1986 to 1987. Results The study investigated a total of 5 539 newborns:1 412 in Beijing, 1 410 in Harbin, 1 274 in Changsha and 1 443 in Guangzhou. Except for the head circumference of male newborns, the weight, height and head circumference of full-term newborns in four cities in 2012 were all more than the national neonatal growth standard of 1986-1987 (P<0.05). Compared with the data obtained from 1986-1987, the sex ratio and the prevalence of macrosomia and premature were higher, but the rates of post-term delivery and low birth weight were lower (P<0.01). Conclusions The new-borns in four cities of China are in good condition during recent 25 years. More attention should be paid to improve the birth sex ratio and to decrease rates of macrosomia and premature.
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Bupleurum chinense is a commonly used Chinese medicinal material, which has been used medicinally in China for over 2000 years, the development of it is of great value. There have been great advances of studies on its embryology and seed biology in recent years. In this paper, we make a review of the growth of its embryo and fruit, the characteristics of dormancy and storage and the germination of its seed. Besides, we summarize the reasons of its low germination rate and the achievements in improving the situation, for the purpose of providing reference in research on seed physiology of B. chinense.
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Bupleurum , Chemistry , Physiology , Drugs, Chinese Herbal , Plant Dormancy , Seeds , Chemistry , PhysiologyABSTRACT
OBJECTIVE@#To evaluate the therapeutic effect of endoscopic ethmoid and maxillary surgery on chronic hypertrophic rhinitis.@*METHOD@#A total of 54 cases of chronic hypertrophic rhinitis were treated by endoscopic ethmoid and maxillary surgery between 2003 and 2004, undergoing postoperative follow-up of more than one year. Age of patients ranged from 17 to 60 years, with a mean of 35 years. All of them were identified with nasal endoscopy and CT before surgery.@*RESULT@#Fifty (92.59%) of 54 cases showed their nasal obstruction symptoms were relieved completely or improved significantly, with nearly normal infraturbinal appearance.@*CONCLUSION@#Endoscopic ethmoid and maxillary surgery is an effective approach for the treatment of chronic hypertrophic rhinitis, with good preservations of infraturbinal structure and function.
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Adolescent , Adult , Female , Humans , Male , Middle Aged , Young Adult , Chronic Disease , Endoscopy , Methods , Ethmoid Sinus , General Surgery , Ethmoid Sinusitis , General Surgery , Hypertrophy , Maxillary Sinus , General Surgery , Maxillary Sinusitis , General Surgery , Rhinitis , General Surgery , Treatment OutcomeABSTRACT
Objective To study the expression of Livin and its relationship with Caspase-3, FHIT in skin squamous cell carcinoma. Methods lmmunohistochemical assay was used to detect the expression of Livin, Caspase-3 and FHIT in 48 cases of sSCC, 20 cases of chronic ulcer and 12 cases of normal skin tissues. Results The rate of positive expression of Livin in SCC tissues was significantly higher than that in the chronic ulcer tissues and normal skin tissues (P <0.01). The rate of positive expression of Caspase-3 and FHIT in SCC tissues was significantly lower than that in the chronic ulcer tissues and normal skin tissues (P < 0.01).The expression of Livin and Caspase-3 had relationship with the lymphatic metastasis and histologic differentiation of SCC(P <0.05). The expression of Livin and Caspase-3 had not relationship with age, sex, the sizes of SCC (P >0.05). There was negative significant correlation between the expression of Livin and the expression of Caspase-3, FHIT. Conclusion There may be relationship between the abnormal expression of Livin and Caspase-3, FHIT and the occurrence, progression of skin squamous cell carcinomas. They may become a new method for preservation and treatment skin of squamous cell carcinomas.
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Objective By researching the auditory brainstem response(ABR) latency and interpeak latency intervals before and after continuous positive airway pressure through the nares(nCPAP), the experiment explored the pathophysiological and anatomical basis of electric physiological change in patients with obstructive sleep apnea/hypopnea syndrome(OSAHS).Methods The ABR of 45 OSAHS patients(90 ears) and control group(32 ears)was examined,the sleep of patients with OSAHS was monitored by polysomnography(PSG).The results of ABR before and after nCPAP were compared.Results 1.The latency of wave III? V and interpeak latency intervals of I-III,III-V,I-V of patients were longer than that of the control group.2.After nCPAP,wave III and interpeak latency intervals of I-III,III-V changed obviously.Conclusion 1.The OSAHS patients' auditory brainstem structure is impaired,which has relationship with high carbonic acid and low oxygen saturation. 2.Except wave III,produced by upper nucleus olivaris that is near the breathing center,effect of nCPAP on ABR of patients with OSAHS is not obvious.