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Objective To compare the diagnostic efficacies of narrow-band imaging (NBI) in distinguishing neoplastic from non-neoplastic colorectal lesions with routine endoscopy and with magnifying endoscopy. Methods Patients with colorectal lesions detected by NBI from September 2008 to February 2010 were enrolled in the study. These lesions were classified by pit pattern and capillary pattern, which was then assessed by reference to histopathology. Results A total of 100 patients with colorectal lesions were enrolled, and the lesions were observed by NBI with ordinary endoscopy (n =64) and NBI with magnifying endoscopy (n =36), respectively, and 7 cases (5 in NBI with ordinary endoscopy and 2 in NBI with magnifying endoscopy) which did not meet the diagnostic criteria were excluded. The overall diagnostic accuracy of NBI endoscopy in distinguishing neoplastic from non-neoplastic colorectal lesions was 91.4% ( 85/93 ), in which NBI with ordinary endoscopy and magnifying endoscopy was 89. 8% (53/59) and 94. 1% (32/34),respectively, with both significantly higher than that of conventional colonoscopy reported in the literature (79. 1% ) (P ＜ 0. 05 ). However, no significant difference was detected between 2 methods ( P ＞ 0. 05 ).Conclusion Similar with NBI magnifying endoscopy, NBI endoscopy without high magnification may also be useful to distinguish neoplastic from non-neoplastic colorectal lesions.
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potential use in clinical therapy and prognosis estimation of the tumor.
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Objective To investigate whether tumor necrosis factor (TNF)a, TNFb and TNFc microsatellite polymorphisms correlate with chronic atrophic gastritis and gastric adenocarcinoma in Chinese Han population. Methods TNFa, TNFb and TNFc microsatellite alleles in 164 healthy subjects, 53 patients with chronic atrophic gastritis and 56 patients with gastric adenocarcinoma were typed using PCR technique combined with High Voltage denaturing PAGE and silver staining. At the same time, the PCR products were cloned and sequenced. Results The frequency of TNFa10 allele was significantly higher in patients with chronic atrophic gastritis than in healthy individuals ( 19.81% vs. 11.89% , P = 0.04 ). However it was not related to age, gender, degree of atrophy or intestinal metaplasia in patients with chronic atrophic gastritis. The frequency of TNFa6b5c1 haplotype homozygote was significantly lower in patients with gastric adenocarcinoma than in healthy individuals ( 1.79% vs. 15.85% , P = 0.006 ). The sequence result revealed that the copy number of dinucleiotide repeating within the same TNFa allele was not consistent with that in the reports from Western countries. Conclusions It should be more accurate and clear to define TNFa alleles. TNFa10 allele is associated with the susceptibility to chronic atrophic gastritis. TNFa6b5c1 haplotype homozygote is negatively associated with gastric adenocarcinoma and thus may play a resistant role in the shifting process from chronic atrophic gastritis to gastric adenocarcinoma.
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Objective:The study is to investigate whether macrophage migration inhibitory factor(MIF)-173 single nucleotide polymorphism(SNP) correlates with inflammatory bowel disease(IBD) in Chinese Han population in Zhejiang Province.Methods:MIF-173 SNP alleles were typed using tetra- primer amplification refractory mutation system(ARMS) and restriction fragment length polymorphisms(RFLP)-PCR in 142 healthy subjects and 98 patients with inflammatory bowel disease(IBD),respectively.At the same time,the PCR products were cloned and sequenced.Results:We detected three kinds of genotypes at the MIF-173 locus,no deviation from Hardy-Weinberg equilibrium were observed.The result was the same from tetra-primer ARMS and RFLP-PCR.Statistical analysis showed,the frequency of MIF-173 CC genotype was significantly higher in patients with ulcerative colitis(UC) 15.5% than in healthy individuals 5.6%(P
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Objective:To investigate the distribution of TNF microsatellite polymorphisms in Chinese Han population of Hubei province Methods:DNA samples were extracted from 164 unrelated healthy individuals’EDTA blood TNF microsatellite alleles were typed using PCR technique,followed by High Voltage denaturing PAGE,with silver staining At the same time,the PCR products were cloned and sequenced Results:Detected seven alleles and eleven kinds of genotypes at the TNFb locus;four alleles and seven kinds of genotypes at the TNFe locus The polymorphism information contents (PIC) were 0 67 and 0 33 respectively No deviation from Hardy Weinberg equilibrium were observed Statistical analysis showed,the distribution of TNFb and TNFe alleles in Chinese Han population were significantly different from that in European or in American Caucasian(P