ABSTRACT
@#ObjectiveTo investigate the effect of traumatic brain injury on the expressions of glial cell line-derived neurotrophic factor(GDNF) in brain tissue of rats.MethodsMale Sprague-Dawley rats were divided into normal control, sham surgery and injury groups. The rats of injury groups were subjected to Marmarou's closed traumatic brain injury and then were subdivided into 1 h, 2 h, 4 h, 8 h, 12 h, 24 h, 48 h, 72 h and 5 d subgroups according to the time elapsed after injury. The expressions of GDNF were studied with immunohistochemistry.ResultsIn control group, mild expressions of GDNF were observed in cortex, hippocampus and brain stem of rats. The number of GDNF positive neurons reached the peak level at 2 h in cortex after injury, and lasted for 5 d. In hippocampus and brain stem, the number of that also reached the peak level at 2 h, and lasted for 72 h.ConclusionThe expressions of GDNF increased significantly at the early time in cortex, hippocampus and brain stem of rats after injury. The significant expression of GDNF lasted longer in cortex.
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@# Objective To study the effects of stereotactic technique on hypertensive basal ganglion hemorrhage. Methods 160 patients with hypertensive basal ganglion hemorrhage were divided into 2 groups: surgical group （132 cases）and conservative group （28 cases）. They were assessed with clinical neurologic impairment scale before and 2 weeks and 4 weeks after treatment. The incidence of rehemorrhage was compared. Results The neurologic impairment scores in surgical group and conservative group were (33.90±3.54) and (33.61±3.82) before treatment (P>0.05), (20.89±3.10) and (26.18±3.61) 2 weeks after treatment (P<0.01), (10.28±2.01) and (15.68±3.28) 4 weeks after treatment (P<0.01), respectively. The incidence of rehemorrhage in surgical group and conservative group were 6.1% and 10.7% (P>0.05), respectively. Conclusion The stereotactic technique may recover neurological function much faster.
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@#Objective To investigate the effect of traumatic brain injury on the expressions of glial cell line-derived neurotrophic factor (GDNF) and its receptors in brain stem of rats.Methods 55 male Sprague-Dawley rats were divided into the normal control group, sham surgery group and injury groups. The rats of injury groups were subjected to Marmarou's closed traumatic brain injury and then were subdivided into 1 h, 2 h, 4 h, 8 h, 12 h, 24 h, 48 h, 72 h and 5 d groups according to the time elapsed after injury. The expressions of GDNF and its receptors (GFRα-1 and Ret) were tested with immunohistochemistry.Results Mild expressions of GDNF and its receptors were observed in brain stem of rats in the normal control group and sham surgery group. The number of GDNF positive neurons reached the peak level at 2 h in brain stem after injury, and that of GFRα-1 and Ret positive neurons reached the peak level at 4 h after injury.Conclusion The expressions of GDNF and its receptors increase significantly at the early time in brain stem of rats after injury. The similar temporal patterns of expressions of GDNF and its receptors are observed in brain stem after brain injury.
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@#Objective To investigate the expressions of glial cell line-derived neurotrophic factor(GDNF)and its receptors,GFRα-1 and Ret,in cortex of rats after closed traumatic cerebral injury.Methods Male Sprague-Dawley rats were divided into normal control,sham and injury groups.The rats of injury groups were subjected to Marmarou's closed traumatic cerebral injury and then were subdivided into 1 h,2 h,4 h,8 h,12 h,24 h,48 h,72 h and 5 d groups according to the time elapsed after injury.The expression of GDNF and its receptors were determined with immunohistochemistry.Results Mild expression of GDNF and its receptors were observed in cortex of rats in control groups.The number of GDNF positive neurons reached the peak level in cortex 2 h after injury,and that of GFRα-1 and Ret positive neurons reached the peak level 4 h after injury.Conclusion The expressions of GDNF and its receptors increased significantly at the early time in cortex of rats after injury,as well as its receptors.It suggests that GDNF and its receptors play an important role after traumatic cerebral injury.
ABSTRACT
@# Objective To study the effect of traumatic brain injury on the expression of glial cell line-derived neurotrophic factor(GDNF)and its receptors in hippocampus of rats.Methods Male Sprague-Dawley rats were divided into normal control,sham group and injury groups.The rats of injury groups were subjected to Marmarou's closed traumatic brain injury and then were subdivided into 1 h,2 h,4 h,8 h,12 h,24 h,48 h,72 h and 5 d groups according to the time elapsed after injury.The expression of GDNF and its receptors were measured with immunohistochemistry.Results Mild expression of GDNF and its receptors were observed in hippocampus of rats in control group.The number of GDNF positive neurons reached the peak level in hippocampus 2 h after injury,and that of GFRα-1 and Ret positive neurons reached the peak level 4 h after injury.Conclusion The expression of GDNF and its receptors were increased significantly at the early time in hippocampus of rats after injury in a similar temporal patterns after brain injury.