Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 15 de 15
Filter
1.
Int. j. morphol ; 33(3): 831-834, Sept. 2015. ilus
Article in English | LILACS | ID: lil-762550

ABSTRACT

This study compared anthropometric parameters among different military sports. One hundred and seventy male cadets of the Brazilian Air Force Academy, who were participants of athletics (n= 33, 20.5±1.3 years), basketball (n= 11, 19.9±1.8 years), fencing (n= 10, 19.9±0.7 years), soccer (n= 21, 20.9±1.6 years), judo (n= 17, 20.7±1.0 years), swimming (n= 15, 20.4±1.2 years), orienteering (n= 10, 21.3±1.6 years), military pentathlon (n= 11, 20.7±1.2 years), water polo (n=10, 21.1±0.9 years), shooting (n= 18, 21.3±1.2 years) or volleyball (n= 14, 20.9±1.7 years) military competitive teams, participated in this study. Height, body mass, body mass index, fat percentage, lean body mass, fat mass, waist and hip perimeters, and waist-hip ratio were measured. Main effects were detected for height, body mass, body mass index, lean body mass, waist and hip perimeters. The only significant finding was that orienteers present lower lean body mass than volleyball and water polo players. These results point out the homogeneity of group anthropometric parameters (except lean body mass), suggesting that military coaches do not rely on the anthropometric parameters, but on specific skills demonstrated during initial period of practice to choose athletes for their teams.


Este estudio comparó los parámetros antropométricos entre cadetes que practican diferentes deportes militares. Ciento setenta cadetes hombres de la Academia de la Fuerza Aérea, que eran participantes de equipos militares competitivos de atletismo (n= 33, 20,5±1,3 años), baloncesto (n= 11, 19,9±1,8 años), esgrima (n= 10, 19,9±0,7 años), fútbol (n=21, 20,9±1,6 años), judo (n= 17, 20,7±1,0 años), natación (n= 15, 20,4±1,2 años), orientación (n= 10, 21,3±1,6 años), pentatlón militar (n= 11, 20,7±1,2 años), polo acuático (n= 10, 21,1±0,9 años), tiro (n= 18, 21,3±1,2 años) y voleibol (n= 14, 20,9±1,7 años) participaron en este estudio. Se midió la altura, masa corporal, índice de masa corporal, porcentaje de grasa corporal, masa magra, masa grasa, perímetros de cintura y cadera, y la relación cintura-cadera. No se detectaron variaciones de altura, masa corporal, índice de masa corporal, masa magra corporal, perímetro de cintura y cadera. El único hallazgo significativo fue que los orientadores tenían una masa corporal magra más baja que los jugadores de voléibol y polo acuático. Estos resultados apuntan a la homogeneidad de los parámetros antropométricos en grupos (excepto la masa magra), lo que sugiere que los instructores militares no dependen de los parámetros antropométricos, sino de las habilidades específicas demostradas durante el período de la práctica inicial en la selección de los atletas para sus equipos.


Subject(s)
Humans , Male , Young Adult , Anthropometry , Military Personnel , Sports , Body Composition , Brazil
2.
Rev. bras. cineantropom. desempenho hum ; 15(3): 382-392, May-June 2013. ilus
Article in English | LILACS | ID: lil-671579

ABSTRACT

Paddles and fins are used during swim training and practice as tools for improving performance. The use of these equipment can alter physiological and kinematic parameters of swimming. The purpose of this literature review was to present and discuss the effects of paddles and fins on kinematic and physiological variables in front crawl, and provide update on the topic for teachers, researchers, coaches and swimmers. Thirty articles were reviewed. To crawl, paddles can change the averages of stroke length and stroke rate, the average swimming speed, the absolute duration of the stroke phases and the index of coordination. Fins can modify the average stroke rate, the average swimming speed, the kick frequency and deep, and the energy cost. We found no studies that verified the longitudinal effects of the use of paddles and fins on these parameters.


Palmares e nadadeiras são utilizados durante a prática e o treinamento da natação como ferramentas para a melhora do desempenho. A utilização desses equipamentos pode alterar parâmetros cinemáticos e fisiológicos do nado. Os objetivos dessa revisão de literatura foram apresentar e discutir os efeitos dos palmares e nadadeiras sobre variáveis cinemáticas e fisiológicas no nado crawl e fornecer atualização sobre o tema a professores, pesquisadores, treinadores e nadadores. Trinta artigos foram revisados. Para o nado crawl, palmares podem alterar o comprimento e a frequência média de braçadas, velocidade média de nado, duração absoluta das fases da braçada e índice de coordenação. Nadadeiras podem modificar a frequência média de braçadas, velocidade média de nado, frequência de pernada e profundidade da pernada e custo energético. Não foram encontrados estudos que verificaram os efeitos longitudinais da utilização de nadadeiras e palmares nesses parâmetros.

3.
Arq. bras. neurocir ; 32(2)jun. 2013. ilus
Article in Portuguese | LILACS | ID: lil-681390

ABSTRACT

According to prospective studies, vitreous hemorrhage may be diagnosed in 8%-27% of the patients with aneurysmal subarachnoid hemorrhage (SAH) (Terson?s syndrome) and has been associated with a bad neurological outcome. In spite of its incidence and prognostic value, vitreous hemorrhage is underdiagnosed. We describe the case of a 48 year-old woman who was diagnosed with a SAH due to the rupture of an aneurysm of the right middle cerebral artery bifurcation that was surgically treated with minimal neurological morbidity. However, due to vitreous hemorrhage in the right eye, the patient developed visual loss that did not recovered after proper surgical treatment. In the context of the present case we take a timely review of the literature, discussing the incidence, pathophysiology, treatment and prognosis of Terson?s syndrome. The reported case stresses that the natural history of Terson?s syndrome is not always synonymous with good outcome. Significant visual sequelae due to vitreous hemorrhage are very rare after proper conservative or surgical treatment. The severe visual loss of this patient emphasizes the need for a systematic, early and serial ophthalmological evaluation of all patients with aneurysmal SAH...


De acordo com estudos prospectivos, a hemorragia do vítreo pode ser diagnosticada em 8%-27% dos doentes com hemorragia subaracnóidea aneurismática (síndrome de Terson) e foi associada a mau prognóstico neurológico. Apesar da sua incidência e valor prognóstico, a hemorragia do vítreo é subdiagnosticada. Os autores descrevem o caso de uma doente de 48 anos, diagnosticada com hemorragia subaracnóidea por rotura de um aneurisma da bifurcação da artéria cerebral média direita, que foi tratado cirurgicamente com mínima morbilidade neurológica. Contudo, por causa da hemorragia do vítreo do olho direito, a doente desenvolveu perda de visão, que não recuperou após tratamento adequado. No contexto do presente caso, revimos a literatura e discutimos a incidência, a fisiopatologia, o diagnóstico e o prognóstico da síndrome de Terson. O caso descrito enfatiza que a história natural da síndrome de Terson não é necessariamente sinônimo de boa evolução. Sequelas visuais significativas após hemorragia do vítreo são muito raras após tratamento conservador ou cirúrgico. A importante perda de visão dessa doente enfatiza a necessidade de avaliação oftalmológica sistemática, precoce e seriada de todos os doentes com hemorragia subaracnóidea aneurismática...


Subject(s)
Humans , Female , Middle Aged , Eye Diseases , Subarachnoid Hemorrhage/surgery , Subarachnoid Hemorrhage/complications , Vitreous Body
4.
Rev. bras. educ. fís. esp ; 26(1): 37-45, jan.-mar. 2012. ilus, graf, tab
Article in Portuguese | LILACS | ID: lil-624570

ABSTRACT

O objetivo deste estudo foi analisar a reprodutibilidade dos parâmetros biomecânicos da curva força-tempo do estilo "Crawl" em um protocolo de 10 s no nado atado. Dezesseis nadadores do sexo masculino (idade: 20,4 ± 4,0 anos; tempo na prova de 100 m livre: 53,68 ± 0,99 s) realizaram dois esforços máximos de 10 s no nado atado. Os parâmetros força pico, força média, taxa de desenvolvimento de força, impulso, duração da braçada, tempo para atingir a força pico e força mínima foram representados pela média de oito braçadas consecutivas obtidas em cada tentativa. Utilizou-se o teste t para observar as diferenças entre os esforços para cada parâmetro. O nível de significância estabelecido foi de 5%. A reprodutibilidade relativa foi medida pelo coeficiente de correlação de Pearson e a consistência entre as duas tentativas pelo coeficiente de correlação intraclasse (CCI). A reprodutibilidade absoluta foi verificada pelo coeficiente de variação (CV). Não foi demonstrada diferença estatisticamente significante para nenhum parâmetro biomecânico quando comparados os dois esforços. Os elevados CCI e baixos CV indicaram alta consistência interna dos parâmetros analisados. Conclui-se que os parâmetros biomecânicos analisados a partir do nado atado são reprodutíveis quando empregado protocolo de curta duração o que demonstra a possibilidade de utilização do protocolo com alto grau de confiabilidade, por parte de treinadores e atletas.


The aim of the present study was to analyze the reliability of biomechanical parameters of the front-Crawl's force-time curve in a 10-s protocol. Sixteen national competitive male swimmers (20.4 ± 4.0 years; 100-m best time: 53.68 ± 0.99 s) performed two 10-s maximal efforts in tethered swimming. Peak force, average force, impulse, rate of force development, stroke duration, time to peak force and minimum force were represented by the mean of eight consecutive strokes obtained in each trial. The paired student-t test was used to verify differences between trials for each biomechanical parameter. The significance level adopted was 5%. Relative reliability was tested by Pearson correlation coefficient and the consistency between trials by means of intraclass coefficient (CCI). The coefficient of variation was used to verify the absolute reliability. No significant differences between the trials to any biomechanical parameter were found. High CCI values and low CV values revealed the high internal consistency of the analyzed variables. It can be concluded that the biomechanical parameters regarding the tethered swimming are reliable in short duration protocols and may be used by coaches and athletes for monitoring training.


Subject(s)
Humans , Male , Adult , Physical Exertion , Swimming , Biomechanical Phenomena
5.
Rev. bras. oftalmol ; 70(5): 303-305, out. 2011. ilus
Article in Portuguese | LILACS | ID: lil-606741

ABSTRACT

O Hemangioma racemoso da retina é uma malformação congênita caracterizada por comunicações arteriovenovas (AV) retinianas. Em cerca de 30 por cento dos casos pode haver manifestações a nível do sistema nervoso central (SNC), denominando-se então por Síndrome de Wyburn-Mason. Os autores apresentam um caso clínico de um doente do sexo masculino de 39 anos que recorre ao serviço de Urgência devido a diminuição da acuidade visual do olho esquerdo (OE) com cerca de seis dias de evolução.Apresenta melhor acuidade visual corrigida olho direito (OD): 4/10 e olho esquerdo (OE): 2/10.À fundoscopia apresenta disco óptico com limites indefinidos, alguma palidez, dilatação e tortuosidade, AV marcada bilateralmente. O hemangioma racemoso da retina é um diagnóstico clínico raro, efectuado por meio do exame oftalmológico. É importante verificar o envolvimento do SNC por meio da realização de angiorressonância magnética nuclear. Atualmente não existe nenhum tratamento preconizado, sendo importante a realização de observações periódicas para averiguação de eventuais complicações.


Racemose Haemangioma (RH) is a congenital anomaly, characterized by retinal arteriovenous(AV) malformation. About 30 percent of patients have signs of central nervous system (CNS) involvement, which has been called the Wyburn-Mason syndrome. The authors present a 39-year-old male patient, who was admitted at the emergency room with decreased visual acuity of his left eye, for the last 6 days. Ophthalmic examination was performed and showed a visual acuity of 4/10 in his right eye and of 2/10 in the left eye. Fundoscopy examination revealed pallor of both optic discs, with blurred limits and a characteristic dilation and AV connections. RH of retina is a rare entity which can be diagnosed by ophthalmic examination. Once this diagnosis is established, involvement of CNS has to be rulled out performing an angioMRI. Currently, no specific treatment has been defined, except a periodic follow-up to prevent complications.


Subject(s)
Humans , Male , Adult , Arteriovenous Malformations/diagnosis , Fluorescein Angiography , Retinal Neoplasms/diagnosis , Hemangioma/diagnosis , Arteriovenous Malformations/classification , Retina/diagnostic imaging , Visual Acuity , Ultrasonography , Tomography, Optical Coherence , Fundus Oculi
6.
Rev. bras. cineantropom. desempenho hum ; 9(4)out.-dez. 2007. tab, graf
Article in Portuguese | LILACS | ID: lil-496701

ABSTRACT

O objetivo deste estudo foi verificar os efeitos do treinamento de força na relação força muscular fora da águadesempenho aeróbio dentro da água. Dezesseis nadadores foram divididos em grupos controle (GC) e experimental (GE), e submetidos a dezessete semanas de treinamento dentro da água. O GE também realizou um treinamento de força fora daágua (TFFA), objetivando o ganho de potência. Antes e após as doze semanas do TFFA foi aplicado o teste de 10 minutos (T10) para avaliar a resistência aeróbia, 1 ação voluntária máxima (1AVM) e repetições máximas em 30s (REM) com 70% da carga de 1AVM, de onde foi calculada a quilagem total. Valores obtidos em 1AVM e quilagem foram relativizados pela massa corporal. Não foi observada correlação signifi cante entre as alterações percentuais do T10 e dos indicadores de força muscular fora da água, apesar do aumento significante deles no GE. Conclui-se que o TFFA não gera melhora no Desempenho aeróbio dentro da água...


The aim of this study was to verify the effects of strength training on the muscular strength-aerobic performancerelationship. Sixteen swimmers were assigned to control (CG) or experimental (EG) groups and underwent seventeen weeks of swimming training. Swimmers in the EG also performed dry land strength training (DLST), lasting twelve weeks,and aiming to develop power. A 10 minutes test (T10) was taken before and after the 12 weeks of DLST, in order to evaluate aerobic resistance, one maximum voluntary contraction (1MVC) and maximum repetition in 30s (REM) using 70% of 1MVC, where total weight was calculated. Total weight, T10, and 1MVC were all normalized for body mass. There was no signifi cantcorrelation between percent changes in T10 and dry land muscle strength, despite signifi cant increases being detected in dry land muscle strength. It can be concluded that nonspecifi c training does not improve aerobic performance in swimming...


Subject(s)
Biomechanical Phenomena , Psychomotor Performance , Swimming
7.
Mem. Inst. Oswaldo Cruz ; 101(8): 833-843, Dec. 2006. ilus
Article in English | LILACS | ID: lil-440569

ABSTRACT

The horizontal transfer of Trypanosoma cruzi mitochondrial minicircle DNA to the genomes of naturally infected humans may play an important role in the pathogenesis of Chagas disease. Minicircle integrations within LINE-1 elements create the potential for foreign DNA mobility within the host genome via the machinery associated with this retrotransposon. Here we document integration of minicircle DNA fragments in clonal human macrophage cell lines and their mobilization over time. The movement of an integration event in a clonal transfected cell line was tracked at three months and three years post-infection. The minicircle sequence integrated into a LINE-1 retrotransposon; one such foreign fragment subsequently relocated to another genomic location in association with associated LINE-1 elements. The p15 locus was altered at three years as a direct effect of minicircle/LINE-1 acquisition, resulting in elimination of p15 mRNA. Here we show for the first time a molecular pathology stemming from mobilization of a kDNA/LINE-1 mutation. These genomic changes and detected transcript variations are consistent with our hypothesis that minicircle integration is a causal component of parasite-independent, autoimmune-driven lesions seen in the heart and other target tissues associated with Chagas disease.


Subject(s)
Humans , Animals , DNA, Kinetoplast/genetics , Gene Expression/genetics , Long Interspersed Nucleotide Elements/genetics , Retroelements/genetics , Trypanosoma cruzi/genetics , Cell Line/parasitology , Gene Transfer, Horizontal , Host-Parasite Interactions/genetics , Macrophages/parasitology , Trypanosoma cruzi/physiology
8.
Braz. j. infect. dis ; 10(3): 169-172, June 2006. ilus, tab
Article in English | LILACS | ID: lil-435281

ABSTRACT

As various types of human papillomavirus (HPV) are involved in the pathogenesis of cervical cancer, correct diagnosis is of fundamental importance for screening programs. We evaluated the divergence of results between Papanicolaou cytology and hybrid capture by PCR detection of HPV DNA . A transversal study was conducted on 70 women attending private gynecological clinics in Brasilia, Brazil. PCRs were conducted with specific primers for general and high-risk HPV DNA. Based on the PCR results, hybrid capture was a superior diagnostic technique. When Papanicolaou was compared with the molecular biology methods, it was found that a positive Papanicolaou result does not necessarily indicate the presence of HPV. The agreement between PCR and hybrid capture results can be attributed to the fact that both methods detect latent infection, while Papanicolaou detects only microscopic cellular alterations.


Subject(s)
Adult , Aged , Female , Humans , Middle Aged , DNA, Viral/analysis , Polymerase Chain Reaction , Papillomaviridae/isolation & purification , Papillomavirus Infections/diagnosis , Vaginal Smears , Cross-Sectional Studies , Papillomaviridae/genetics , Papillomavirus Infections/pathology , Papillomavirus Infections/virology , Sensitivity and Specificity
9.
J. bras. pneumol ; 32(2): 123-129, mar.-abr. 2006. tab, graf
Article in Portuguese | LILACS | ID: lil-433228

ABSTRACT

OBJETIVO: Avaliar o efeito do método Reequilíbrio Toracoabdominal na força dos músculos respiratórios de pacientes com fibrose cística, acompanhados no Ambulatório de Fibrose Cística da Universidade Católica de Brasília. MÉTODOS: A amostra, constituída de 29 fibrocísticos, foi caracterizada com base em dados antropométricos, genéticos e de colonização bacteriana. Espirometria, manovacuometria e antropometria foram realizadas antes e depois do tratamento fisioterapêutico, no qual se utilizou o método Reequilíbrio Toracoabdominal, duas vezes por semana, durante quatro meses. RESULTADOS: Houve aumento da pressão inspiratória máxima e da pressão expiratória máxima após o tratamento fisioterapêutico em todos os pacientes, naqueles sem distúrbio ventilatório obstrutivo e naqueles com distúrbio ventilatório obstrutivo leve (p < 0,05). Foi encontrada correlação positiva entre a idade e a pressão expiratória máxima para a maioria dos grupos. A pressão inspiratória máxima só apresentou correlação positiva com a idade no grupo com distúrbio ventilatório obstrutivo leve (p = 0,012; r = 0,817). Para o sexo feminino e para o grupo sem distúrbio ventilatório obstrutivo houve correlação negativa entre a pressão expiratória máxima e a colonização por Pseudomonas aeruginosa (p = 0,036; r = -0,585). CONCLUSÃO: Para os fibrocísticos avaliados, o método Reequilíbrio Toracoabdominal aumentou a força dos músculos respiratórios, o que reafirma a importância do tratamento fisioterapêutico para estes pacientes.


Subject(s)
Humans , Male , Female , Child , Adolescent , Adult , Abdominal Muscles/physiology , Breathing Exercises , Cystic Fibrosis/rehabilitation , Muscle Strength/physiology , Respiratory Muscles/physiology , Body Mass Index , Cystic Fibrosis/genetics , Cystic Fibrosis/microbiology , Forced Expiratory Volume/physiology , Genotype , Pseudomonas aeruginosa/isolation & purification , Severity of Illness Index , Spirometry , Treatment Outcome , Vital Capacity
10.
Rev. bras. educ. fís. esp ; 20(2): 141-150, abr.-jun. 2006. tab, graf
Article in Portuguese | LILACS | ID: lil-469671

ABSTRACT

O objetivo deste trabalho foi verificar se a utilização do treinamento de força fora da água influenciou o desempenho de nadadores. Dezesseis nadadores (21,93 anos mais ou menos 2,17) foram aleatoriamente dividido sem grupos Controle (GC / n = 8) e Experimental (GE / n = 8), realizando o mesmo tipo de treinamento. GE também foi submetido ao treinamento de força objetivando o ganho de potência. As avaliações ocorreram na terceira (Pré) e na décima sétima (Pós) semana. Dentro da água a velocidade média foi coletada nas distâncias de 25 (T25) e 50 m (T50). Fora da água foram utilizados os testes de uma repetição máxima (1RM) e repetições máximas com 70% de 1RM (RSM) no período de 30 segundos para supino reto fechado (SRF), remada alta (RA) e leg press inclinado (LPI). Alterações Pré e Pós foram detectadas pela análise de variância por medidas repetidas (ANOVA) seguidas pelo post-hoc de Scheffé (p < 0,05). T25 apresentou diferenças significantes de Pré para Pós em ambos os grupos (5,21% para o GC e 3,74% para o GE). Para T50 também houve diferenças de Pré para Pós para ambos os grupos(5,69% e 5,33% para GC e GE, respectivamente). No teste de 1RM, o GE apresentou melhoras (p < 0,05) de 16,47% no SRF, 17,34% na RA e 25,31%. Já o GC apresentou alteração significante apenas no LPI (14,01%). RSM não apresentou alterações significantes em nenhum dos grupos. Apesar do aumento da força avaliada fora da água no GE, o desempenho foi melhor no GC. Conclui-se que, a metodologia aplicada ao treinamento de força não influenciou positivamente o desempenho dentro da água.


Subject(s)
Humans , Male , Adult , Sports , Swimming , Outflow Velocity Measurement
11.
Braz. j. infect. dis ; 6(3): 110-118, Jun. 2002.
Article in English | LILACS | ID: lil-332325

ABSTRACT

The notification of sexually transmitted diseases (STD) is a prime component of well-designed public health policy. However, peculiar aspects of STD must be taken into account for the correct management of surveillance activities. Here, we describe the distribution of the most common sexually transmitted diseases among patients attended by the gynecological clinics of the principal public hospitals of Brasilia and the Federal District, Brazilian capital, during six years. A total of 142,158 patients had their cervicovaginal samples collected for Papanicolaou preparations and eventual biopsies. Diagnosis was made according to cytological and histological alterations, distinguishing among vaginal infections, and pre-cancerous and cancerous cervical lesions. We also looked at the annual prevalence of the various types of infections and alterations. There was a high prevalence of bacterial vaginosis, trichomoniasis and candidiasis, with suggestive changes over the years. Pre-cancerous and cancerous lesions increased 2.2 fold during the six years. A large proportion of the cases involved late stages of cervical cancer, indicating the necessity of prompt attendance of the population in a routine gynecological prevention program.


Subject(s)
Animals , Female , Humans , Sexually Transmitted Diseases/epidemiology , Health Surveys , Brazil , Candida , Candidiasis/epidemiology , Candidiasis/microbiology , Chlamydia trachomatis , Sexually Transmitted Diseases/diagnosis , Sexually Transmitted Diseases/pathology , Sexually Transmitted Diseases/prevention & control , Follow-Up Studies , Gardnerella vaginalis , Herpes Genitalis , Tumor Virus Infections/epidemiology , Mass Screening , Papillomaviridae , Prevalence , Trichomonas vaginalis , Trichomonas Vaginitis , Uterine Cervical Neoplasms , Uterine Neoplasms , Vaginal Smears , Vaginosis, Bacterial
12.
Rev. Inst. Med. Trop. Säo Paulo ; 43(4): 209-212, Aug. 2001.
Article in English | LILACS | ID: lil-298684

ABSTRACT

We all hope that biotechnology will answer some social and economical unavoidable requirements of the modern life. It is necessary to improve agriculture production, food abundance and health quality in a sustainable development. It is indeed a hard task to keep the progress on taking into account the rational use of genetic resources and the conservation of biodiversity. In this context, a historical perspective and prospects of the biomedical research on parasitic diseases is described in a view of three generations of investigators. This work begins with a picture of the scientific progress on biomedical research and human health over the last centuries. This black-and-white picture is painted by dissecting current advancements of molecular biology and modern genetics, which are outlined at the meaning of prospecting achievements in health science for this new millenium


Subject(s)
Humans , Animals , History, 19th Century , History, 20th Century , Parasitic Diseases , Research/history , Public Health/history , Biotechnology , Research/trends , Public Health/trends , Genetic Techniques/trends
13.
Mem. Inst. Oswaldo Cruz ; 95(supl.1): 123-31, 2000.
Article in English | LILACS | ID: lil-274871

ABSTRACT

The development of biotechnology in the last three decades has generated the feeling that the newest scientific achievements will deliver high standard quality of life through abundance of food and means for successfully combating diseases. Where the new biotechnologies give access to genetic information, there is a common belief that physiological and pathological processes result from subtle modifications of gene expression. Trustfully, modern genetics has produced genetic maps, physical maps and complete nucleotide sequences from 141 viruses, 51 organelles, two eubacteria, one archeon and one eukaryote (Saccharomices cerevisiae). In addition, during the Centennial Commemoration of the Oswaldo Cruz Institute the nearly complete human genome map was proudly announced, whereas the latest Brazilian key stone contribution to science was the publication of the Shillela fastidiosa genomic sequence highlythed on a Nature cover issue. There exists a belief among the populace that further scientific accomplishments will rapidly lead to new drugs and methodological approaches to cure genetic diseases and other incurable ailments. Yet, much evidence has been accumulated, showing that a large information gap exists between the knowledge of genome sequence and our knowledge of genome function. Now that many genome maps are available, people wish to know what are we going to do with them. Certainly, all these scientific accomplishments will shed light on many more secrets of life. Nevertheless, parsimony in the weekly announcements of promising scientific achievements is necessary. We also need many more creative experimental biologists to discover new, as yet un-envisaged biotechnological approaches, and the basic resource needed for carrying out mile stone research necessary for leading us to that "promised land" often proclaimed by the mass media


Subject(s)
Humans , Animals , Biotechnology/trends , Genome, Protozoan , Parasitic Diseases/genetics , Research/trends , Chromosome Mapping , Genome , Host-Parasite Interactions/genetics
14.
Mem. Inst. Oswaldo Cruz ; 94(suppl.1): 249-52, Sept. 1999. ilus
Article in English | LILACS | ID: lil-245631

ABSTRACT

Integration of kDNA sequences within the genome of the host cell shown by PCR amplification with primers to the conserved Trypanosoma cruzi kDNA minicircle sequence was confirmed by Southern hybridization with specific probes. The cells containing the integrated kDNA sequences were then perpetuated as transfected macrophage subclonal lines. The kDNA transfected macrophages expressed membrane antigens that were recognized by antibodies in a panel of sera from ten patients with chronic Chagas disease. These antigens barely expressed in the membrane of uninfected, control macrophage clonal lines were recognized neither by factors in the control, non-chagasic subjects nor in the chagasic sera. This finding suggests the presence of an autoimmune antibody in the chagasic sera that recognizes auto-antigens in the membrane of T. cruzi kDNA transfected macrophage subclonal lines.


Subject(s)
Humans , Animals , Autoimmunity , Chagas Disease/immunology , DNA, Kinetoplast , Trypanosoma cruzi , Base Sequence , Chagas Disease/blood , Genome , Macrophages , Trypanosoma cruzi/genetics
SELECTION OF CITATIONS
SEARCH DETAIL