Protocolo com nove dias de progesterona para inseminação artificial em tempo fixo em vacas taurinas adaptadas ao clima tropical / Protocol with nine days of progesterone for fixed-time artificial insemination in Bos taurus cows adapted to the tropical weather

Três experimentos foram realizados para adaptar um protocolo de sincronização de estro e da ovulação para ser utilizado em programas de inseminação artificial em tempo fixo (IATF) em vacas taurinas tropicalmente adaptadas. No Exp. 1 (crossover), vacas pluríparas Curraleiro Pé-Duro (n= 12) receberam um dispositivo intravaginal contendo 1g de P4 por oito dias e 2mg de BE intramuscular (IM) no momento da inserção do dispositivo (dia 0). No dia da remoção do dispositivo (dia 8), as fêmeas receberam 150µg de D-cloprostenol sódico e 300UI de gonadotrofina coriônica equina (eCG) IM, sendo, então, divididas aleatoriamente para receber 1mg de BE no dia 8 (BE8) ou 1mg de BE no dia 9 (BE9). A aplicação de BE no D9 atrasou a ovulação em aproximadamente 15 horas (P<0,05). No Experimento 2, foram avaliados protocolos com oito (P4D8) e nove dias (P4D9) de exposição à progesterona, resultando em parâmetros de desenvolvimento folicular e luteal semelhantes entre os tratamentos (P>0,05). No Experimento 3, os protocolos hormonais de IATF BE8 e P4D9 foram testados para a taxa de prenhez, alcançando 23% (10/43) e 20% (9/45), respectivamente (P>0,05). Embora o grupo P4D9 tenha mostrado avanço na proporção de animais que responderam ao protocolo quando comparado ao protocolo BE8, este não se refletiu em melhora na taxa de prenhez.(AU)

Three experiments were performed to adapt a synchronization protocol of estrus synchronization and ovulation to be used in fixed time artificial insemination programs (FTAI) in tropically adapted Bos taurus cows. In Exp. 1 (crossover) multiparous Curraleiro Pé-Duro cows (n= 12) received an intravaginal device containing 1g of P4 for 8 days and 2mg of EB at the time of device insertion (Day 0). On the P4 device removal (Day 8) females received 150g of D-cloprostenol Sodic and 300IU of equine chorionic gonadotropin (eCG). Then, they were randomly divided to receive 1mg of EB on Day 8 (EB8) or on Day 9 (EB9). EB9 delayed ovulation approximately 15 hours (P<0.05). In Exp. 2, protocols using progesterone for eight (P4D8) or nine days (P4D9) were evaluated, resulting in similar parameters of folicular and luteal development (P>0.05). In Exp. 3, EB8 and P4D9 protocols were used to evaluate the pregnancy rate, reaching 23% (10/43) and 20% (9/45), respectively (P>0.05). Although P4D9 protocol has shown improvement in proportion of animals that responded to the protocol when compared to EB8 protocol, it was not able to improve pregnancy rate.(AU)

Caracterização da curva de crescimento de fêmeas Crioulas Lageanas criadas em condições naturais / Growth curve characterization of Crioula Lagena cows raised under natural conditions

Morphometry and ultrasound of carcasses were used to estimate the growth curve of Criola Lageana cows, raised under natural conditions. A total of 111 cows, raised under extensively production system in Central Plateau of Santa Catarina, were evaluated. Morphometrics and ultrasound measurements were related with the age of the animals using the targeted univariate regression model, assigning the variable response to gamma distribution. The inflection points of the growth curve were 24 and 25 months for withers height and hip height, between 27 and 29 for weight, body length, rump length, thorax perimeter, depth and distance between ilea. For ribeye area (REA), inflection point was 18 months. The Crioula Lageana cows presented greater growth velocity around 27 months, showing good productive performance under the natural conditions of the Central Plateau of Santa Catarina.(AU)

Association of HDL cholesterol and triglycerides with mortality in patients with heart failure

It has been demonstrated that there is an association between serum lipoproteins and survival rate in patients with ischemic cardiomyopathy, as well as in patients with non-ischemic causes of heart failure. We tested the hypothesis of an association between serum lipoprotein levels and prognosis in a cohort of outpatients with heart failure, including Chagas' heart disease. The lipid profile of 833 outpatients with heart failure in functional classes III and IV of the New York Heart Association, with a mean age of 46.9 ± 10.6 years, 655 (78.6 percent) men and 178 (21.4 percent) women, was studied from April 1991 to June 2003. The survival rate was estimated by the Kaplan-Meyer's method and the Cox proportional hazards models. Etiology of heart failure was ischemic cardiomyopathy in 171 (21 percent) patients, Chagas' heart disease in 144 (17 percent), hypertensive cardiomyopathy in 136 (16 percent), and other etiologies in 83 (10 percent). In 299 (36 percent) patients, heart failure was ascribed to idiopathic dilated cardiomyopathy. Variables significantly associated with mortality were age (hazard ratio, HR = 1.02; 95 percentCI = 1.01-1.03; P = 0.0074), male gender (HR = 1.77; 95 percentCI = 1.2-2.62; P = 0.004), idiopathic dilated cardiomyopathy (HR = 1.81; 95 percentCI = 1.16-2.82; P = 0.0085), serum triglycerides (HR = 0.97; 95 percentCI = 0.96-0.98; P < 0.0001), and HDL cholesterol (HR = 0.99; 95 percentCI = 0.99-1.0; P = 0.0280). Therefore, higher serum HDL cholesterol and higher serum triglycerides were associated with lower mortality in this cohort of outpatients with heart failure.